Infeksi Plasmodium berghei dan Kesannya Keatas pengisyaratan MAP Kinase Eritrosit Perumah

The present investigation involves monitoring the development of parasitemia and the distribution of Plasmodium berghei morphologies during parasite infection in mice as well as the effect P. berghei infection on MAP kinase signaling in its erythrocyte host. Microscopic analyses of Giemsa-stained bl...

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Main Authors:	Mohd.Fakharul Zaman Raja Yahya, -, Hasidah Mohd sidek, -
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2009
Online Access:	http://journalarticle.ukm.my/8/ http://journalarticle.ukm.my/8/ http://journalarticle.ukm.my/8/1/1.pdf

id	ukm-8
recordtype	eprints
spelling	ukm-82016-12-14T06:26:10Z http://journalarticle.ukm.my/8/ Infeksi Plasmodium berghei dan Kesannya Keatas pengisyaratan MAP Kinase Eritrosit Perumah Mohd.Fakharul Zaman Raja Yahya, - Hasidah Mohd sidek, - The present investigation involves monitoring the development of parasitemia and the distribution of Plasmodium berghei morphologies during parasite infection in mice as well as the effect P. berghei infection on MAP kinase signaling in its erythrocyte host. Microscopic analyses of Giemsa-stained blood films prepared from P. berghei (strain PZZ1/00)-infected mice showed that the level of parasitemia reached up to 70% two weeks after inoculation of the parasite. Parasite ring and trophozoite forms were clearly detected in the blood films throughout the study period while the schizont form was visibly observed on day 3 post-inoculation. Western blotting [primary antibody: monoclonal anti-MAP kinase (non-phosphorylated ERK-1/2); secondary antibody: HRP-conjugated polyclonal anti-IgG] carried out on SDS-PAGE-separated cytosolic protein samples from P. berghei-infected (70% parasitemia) erythrocytes showed that the levels of both 42 and 44 kDa MAP kinase-immunoreactive proteins increased significantly (p<0.05) at 70% parasitemia, by up to 21.5% and 22.3% respectively as compared to non-infected control samples. Whether the effect of P. berghei infection at 70% parasitemia on host MAP kinase signaling is related to the MAP kinase activation remains to be further investigated Universiti Kebangsaan Malaysia 2009-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8/1/1.pdf Mohd.Fakharul Zaman Raja Yahya, - and Hasidah Mohd sidek, - (2009) Infeksi Plasmodium berghei dan Kesannya Keatas pengisyaratan MAP Kinase Eritrosit Perumah. pp. 761-766. ISSN 0126-6039 http://pkukmweb.ukm.my/~jsm/kandungan.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The present investigation involves monitoring the development of parasitemia and the distribution of Plasmodium berghei morphologies during parasite infection in mice as well as the effect P. berghei infection on MAP kinase signaling in its erythrocyte host. Microscopic analyses of Giemsa-stained blood films prepared from P. berghei (strain PZZ1/00)-infected mice showed that the level of parasitemia reached up to 70% two weeks after inoculation of the parasite. Parasite ring and trophozoite forms were clearly detected in the blood films throughout the study period while the schizont form was visibly observed on day 3 post-inoculation. Western blotting [primary antibody: monoclonal anti-MAP kinase (non-phosphorylated ERK-1/2); secondary antibody: HRP-conjugated polyclonal anti-IgG] carried out on SDS-PAGE-separated cytosolic protein samples from P. berghei-infected (70% parasitemia) erythrocytes showed that the levels of both 42 and 44 kDa MAP kinase-immunoreactive proteins increased significantly (p<0.05) at 70% parasitemia, by up to 21.5% and 22.3% respectively as compared to non-infected control samples. Whether the effect of P. berghei infection at 70% parasitemia on host MAP kinase signaling is related to the MAP kinase activation remains to be further investigated
format	Article
author	Mohd.Fakharul Zaman Raja Yahya, - Hasidah Mohd sidek, -
spellingShingle	Mohd.Fakharul Zaman Raja Yahya, - Hasidah Mohd sidek, - Infeksi Plasmodium berghei dan Kesannya Keatas pengisyaratan MAP Kinase Eritrosit Perumah
author_facet	Mohd.Fakharul Zaman Raja Yahya, - Hasidah Mohd sidek, -
author_sort	Mohd.Fakharul Zaman Raja Yahya, -
title	Infeksi Plasmodium berghei dan Kesannya Keatas pengisyaratan MAP Kinase Eritrosit Perumah
title_short	Infeksi Plasmodium berghei dan Kesannya Keatas pengisyaratan MAP Kinase Eritrosit Perumah
title_full	Infeksi Plasmodium berghei dan Kesannya Keatas pengisyaratan MAP Kinase Eritrosit Perumah
title_fullStr	Infeksi Plasmodium berghei dan Kesannya Keatas pengisyaratan MAP Kinase Eritrosit Perumah
title_full_unstemmed	Infeksi Plasmodium berghei dan Kesannya Keatas pengisyaratan MAP Kinase Eritrosit Perumah
title_sort	infeksi plasmodium berghei dan kesannya keatas pengisyaratan map kinase eritrosit perumah
publisher	Universiti Kebangsaan Malaysia
publishDate	2009
url	http://journalarticle.ukm.my/8/ http://journalarticle.ukm.my/8/ http://journalarticle.ukm.my/8/1/1.pdf
first_indexed	2023-09-18T19:01:11Z
last_indexed	2023-09-18T19:01:11Z
_version_	1777403084763299840

Infeksi Plasmodium berghei dan Kesannya Keatas pengisyaratan MAP Kinase Eritrosit Perumah

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