Optimization of Enzymatic Hydrolysis of Tilapia Muscle (Oreochromis niloticus) using Response Surface Methodology (RSM)

Fish protein hydrolysate was prepared from tilapia muscle using commercial Alcalase enzyme. Optimization of enzymatic hydrolysis process for preparing tilapia muscle protein hydrolysates (TMPH) was performed by employing central composite design (CCD) method of response surface methodology (RSM). O-...

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Main Authors:	Jumardi Roslan, Siti Mazlina Mustapa Kamal, Khairul Faezah Md. Yunos, Norhafizah Abdullah
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/8042/ http://journalarticle.ukm.my/8042/ http://journalarticle.ukm.my/8042/1/10_Jumardi_Roslan.pdf

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spelling	ukm-80422016-12-14T06:46:02Z http://journalarticle.ukm.my/8042/ Optimization of Enzymatic Hydrolysis of Tilapia Muscle (Oreochromis niloticus) using Response Surface Methodology (RSM) Jumardi Roslan, Siti Mazlina Mustapa Kamal, Khairul Faezah Md. Yunos, Norhafizah Abdullah, Fish protein hydrolysate was prepared from tilapia muscle using commercial Alcalase enzyme. Optimization of enzymatic hydrolysis process for preparing tilapia muscle protein hydrolysates (TMPH) was performed by employing central composite design (CCD) method of response surface methodology (RSM). O-phtaldialdehyde (OPA) method was employed to calculate the degree of hydrolysis (DH), which is the key parameter for monitoring the reaction of protein hydrolysis. The suggested model equation was proposed based on the effects of pH, temperature, substrate concentration and enzyme concentration on the DH. Optimum enzymatic hydrolysis conditions using Alcalase enzyme were obtained at pH7.5, temperature of 50oC, substrate concentration of 2.5% and enzyme concentration of 4.0%. Under these conditions, the highest value of the DH was achieved at 25.16% after hydrolysing at 120 min. The TMPH was further assessed for their nutritional value with respect to chemical and amino acid compositions. Molecular weight distributions of TMPH were characterized by SDS-PAGE. TMPH contains moderate amount of protein (28.14%) and good nutritive value with respect to the higher total amino acid composition (267.57 mg/g). Glutamic acid, aspartic acid and lysine were the most abundant amino acids present in TMPH with values 42.68, 29.16 and 26.21 mg/g, respectively. Protein hydrolysates from tilapia muscle containing a desirable peptide with low molecular weight which may potentially to be used as functional food products. Universiti Kebangsaan Malaysia 2014-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8042/1/10_Jumardi_Roslan.pdf Jumardi Roslan, and Siti Mazlina Mustapa Kamal, and Khairul Faezah Md. Yunos, and Norhafizah Abdullah, (2014) Optimization of Enzymatic Hydrolysis of Tilapia Muscle (Oreochromis niloticus) using Response Surface Methodology (RSM). Sains Malaysiana, 43 (11). pp. 1715-1723. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Fish protein hydrolysate was prepared from tilapia muscle using commercial Alcalase enzyme. Optimization of enzymatic hydrolysis process for preparing tilapia muscle protein hydrolysates (TMPH) was performed by employing central composite design (CCD) method of response surface methodology (RSM). O-phtaldialdehyde (OPA) method was employed to calculate the degree of hydrolysis (DH), which is the key parameter for monitoring the reaction of protein hydrolysis. The suggested model equation was proposed based on the effects of pH, temperature, substrate concentration and enzyme concentration on the DH. Optimum enzymatic hydrolysis conditions using Alcalase enzyme were obtained at pH7.5, temperature of 50oC, substrate concentration of 2.5% and enzyme concentration of 4.0%. Under these conditions, the highest value of the DH was achieved at 25.16% after hydrolysing at 120 min. The TMPH was further assessed for their nutritional value with respect to chemical and amino acid compositions. Molecular weight distributions of TMPH were characterized by SDS-PAGE. TMPH contains moderate amount of protein (28.14%) and good nutritive value with respect to the higher total amino acid composition (267.57 mg/g). Glutamic acid, aspartic acid and lysine were the most abundant amino acids present in TMPH with values 42.68, 29.16 and 26.21 mg/g, respectively. Protein hydrolysates from tilapia muscle containing a desirable peptide with low molecular weight which may potentially to be used as functional food products.
format	Article
author	Jumardi Roslan, Siti Mazlina Mustapa Kamal, Khairul Faezah Md. Yunos, Norhafizah Abdullah,
spellingShingle	Jumardi Roslan, Siti Mazlina Mustapa Kamal, Khairul Faezah Md. Yunos, Norhafizah Abdullah, Optimization of Enzymatic Hydrolysis of Tilapia Muscle (Oreochromis niloticus) using Response Surface Methodology (RSM)
author_facet	Jumardi Roslan, Siti Mazlina Mustapa Kamal, Khairul Faezah Md. Yunos, Norhafizah Abdullah,
author_sort	Jumardi Roslan,
title	Optimization of Enzymatic Hydrolysis of Tilapia Muscle (Oreochromis niloticus) using Response Surface Methodology (RSM)
title_short	Optimization of Enzymatic Hydrolysis of Tilapia Muscle (Oreochromis niloticus) using Response Surface Methodology (RSM)
title_full	Optimization of Enzymatic Hydrolysis of Tilapia Muscle (Oreochromis niloticus) using Response Surface Methodology (RSM)
title_fullStr	Optimization of Enzymatic Hydrolysis of Tilapia Muscle (Oreochromis niloticus) using Response Surface Methodology (RSM)
title_full_unstemmed	Optimization of Enzymatic Hydrolysis of Tilapia Muscle (Oreochromis niloticus) using Response Surface Methodology (RSM)
title_sort	optimization of enzymatic hydrolysis of tilapia muscle (oreochromis niloticus) using response surface methodology (rsm)
publisher	Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/8042/ http://journalarticle.ukm.my/8042/ http://journalarticle.ukm.my/8042/1/10_Jumardi_Roslan.pdf
first_indexed	2023-09-18T19:51:20Z
last_indexed	2023-09-18T19:51:20Z
_version_	1777406239699894272

Optimization of Enzymatic Hydrolysis of Tilapia Muscle (Oreochromis niloticus) using Response Surface Methodology (RSM)

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