A simple and effective isocratic HPLC method for fast identification and quantification of surfactin

The aim of this study was to establish a simple, accurate and reproducible method for the identification and quantification of surfactin using high-performance liquid chromatography (HPLC). Previously reported method of identification and quantification of surfactin were time consuming and requires...

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Main Authors:	Muhammad Qadri Effendy Mubarak, Abdul Rahman Hassan, Aidil Abdul Hamid, Sahaid Khalil, Mohd Hafez Mohd Isa
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/8242/ http://journalarticle.ukm.my/8242/ http://journalarticle.ukm.my/8242/1/16_Muhammad_Qadri_Effendy.pdf

id	ukm-8242
recordtype	eprints
spelling	ukm-82422016-12-14T06:46:39Z http://journalarticle.ukm.my/8242/ A simple and effective isocratic HPLC method for fast identification and quantification of surfactin Muhammad Qadri Effendy Mubarak, Abdul Rahman Hassan, Aidil Abdul Hamid, Sahaid Khalil, Mohd Hafez Mohd Isa, The aim of this study was to establish a simple, accurate and reproducible method for the identification and quantification of surfactin using high-performance liquid chromatography (HPLC). Previously reported method of identification and quantification of surfactin were time consuming and requires a large quantity of mobile phase. The new method was achieved by application of Chromolith® high performance RP-18 (100 × 4.6 mm, 5 μm) as the stationary phase and optimization of mobile phase ratio and flow rate. Mobile phase consisted of acetonitrile (ACN) and 3.8 mM trifluroacetic acid (TFA) solution of 80:20 ratio at flow rate of 2.2 mL/min was obtained as the optimal conditions. Total elution time of the obtained surfactin peaks was four times quicker than various methods previously reported in the literature. The method described here allowed for fine separation of surfactin in standard sample (98% purity) and surfactin in fermentation broth. Universiti Kebangsaan Malaysia 2015-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8242/1/16_Muhammad_Qadri_Effendy.pdf Muhammad Qadri Effendy Mubarak, and Abdul Rahman Hassan, and Aidil Abdul Hamid, and Sahaid Khalil, and Mohd Hafez Mohd Isa, (2015) A simple and effective isocratic HPLC method for fast identification and quantification of surfactin. Sains Malaysiana, 44 (1). pp. 115-120. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The aim of this study was to establish a simple, accurate and reproducible method for the identification and quantification of surfactin using high-performance liquid chromatography (HPLC). Previously reported method of identification and quantification of surfactin were time consuming and requires a large quantity of mobile phase. The new method was achieved by application of Chromolith® high performance RP-18 (100 × 4.6 mm, 5 μm) as the stationary phase and optimization of mobile phase ratio and flow rate. Mobile phase consisted of acetonitrile (ACN) and 3.8 mM trifluroacetic acid (TFA) solution of 80:20 ratio at flow rate of 2.2 mL/min was obtained as the optimal conditions. Total elution time of the obtained surfactin peaks was four times quicker than various methods previously reported in the literature. The method described here allowed for fine separation of surfactin in standard sample (98% purity) and surfactin in fermentation broth.
format	Article
author	Muhammad Qadri Effendy Mubarak, Abdul Rahman Hassan, Aidil Abdul Hamid, Sahaid Khalil, Mohd Hafez Mohd Isa,
spellingShingle	Muhammad Qadri Effendy Mubarak, Abdul Rahman Hassan, Aidil Abdul Hamid, Sahaid Khalil, Mohd Hafez Mohd Isa, A simple and effective isocratic HPLC method for fast identification and quantification of surfactin
author_facet	Muhammad Qadri Effendy Mubarak, Abdul Rahman Hassan, Aidil Abdul Hamid, Sahaid Khalil, Mohd Hafez Mohd Isa,
author_sort	Muhammad Qadri Effendy Mubarak,
title	A simple and effective isocratic HPLC method for fast identification and quantification of surfactin
title_short	A simple and effective isocratic HPLC method for fast identification and quantification of surfactin
title_full	A simple and effective isocratic HPLC method for fast identification and quantification of surfactin
title_fullStr	A simple and effective isocratic HPLC method for fast identification and quantification of surfactin
title_full_unstemmed	A simple and effective isocratic HPLC method for fast identification and quantification of surfactin
title_sort	simple and effective isocratic hplc method for fast identification and quantification of surfactin
publisher	Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/8242/ http://journalarticle.ukm.my/8242/ http://journalarticle.ukm.my/8242/1/16_Muhammad_Qadri_Effendy.pdf
first_indexed	2023-09-18T19:51:54Z
last_indexed	2023-09-18T19:51:54Z
_version_	1777406275275980800

A simple and effective isocratic HPLC method for fast identification and quantification of surfactin

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