Cyanide degradation by pseudomonas pseudoalcaligenes strain W2 Isolated from mining effluent

Cyanide is highly toxic to the living organisms as it inhibits respiration system in the cell mitochondria. Cyanide is commonly used in gold extraction process and its discharge into the environment not only causes pollution but it also brings harm to the surrounding population. Chemical treatment i...

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Main Authors:	Belinda Tiong, Zaratulnur Mohd Bahari, Nor Sahslin Irwan Shah Lee, Jafariah Jaafar, Zaharah Ibrahim, Shafinaz Shahir
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/8307/ http://journalarticle.ukm.my/8307/ http://journalarticle.ukm.my/8307/1/10_Belinda_Tiong.pdf

id	ukm-8307
recordtype	eprints
spelling	ukm-83072016-12-14T06:46:51Z http://journalarticle.ukm.my/8307/ Cyanide degradation by pseudomonas pseudoalcaligenes strain W2 Isolated from mining effluent Belinda Tiong, Zaratulnur Mohd Bahari, Nor Sahslin Irwan Shah Lee, Jafariah Jaafar, Zaharah Ibrahim, Shafinaz Shahir, Cyanide is highly toxic to the living organisms as it inhibits respiration system in the cell mitochondria. Cyanide is commonly used in gold extraction process and its discharge into the environment not only causes pollution but it also brings harm to the surrounding population. Chemical treatment is expensive and the use of hazardous compound can exacerbate the problem. Biodegradation offers cheap and safe alternative as it overcomes the problems faced by chemical treatment. In this study, indigenous bacteria from mining wastewater were isolated. Cyanide degradation was done via shake flask method. A bacterium, designated W2 was found able to grow in the mining wastewater. 16S rRNA analysis identified the strain as Pseudomonas pseudoalcaligenes which could tolerate up to 39 mg/L cyanide concentration and growth was depleted at 52 mg/L. 60% cyanide degradation was achieved in wastewater containing medium. End-product analysis from high performance liquid chromatography (HPLC) detected formamide implicating the role of cyanide hydratase in cyanide degradation. It can be concluded that P. pseudoalcaligenes is capable of biodegrading cyanide and its potential in wastewater treatment containing cyanide is feasible. Universiti Kebangsaan Malaysia 2015-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8307/1/10_Belinda_Tiong.pdf Belinda Tiong, and Zaratulnur Mohd Bahari, and Nor Sahslin Irwan Shah Lee, and Jafariah Jaafar, and Zaharah Ibrahim, and Shafinaz Shahir, (2015) Cyanide degradation by pseudomonas pseudoalcaligenes strain W2 Isolated from mining effluent. Sains Malaysiana, 44 (2). pp. 233-238. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Cyanide is highly toxic to the living organisms as it inhibits respiration system in the cell mitochondria. Cyanide is commonly used in gold extraction process and its discharge into the environment not only causes pollution but it also brings harm to the surrounding population. Chemical treatment is expensive and the use of hazardous compound can exacerbate the problem. Biodegradation offers cheap and safe alternative as it overcomes the problems faced by chemical treatment. In this study, indigenous bacteria from mining wastewater were isolated. Cyanide degradation was done via shake flask method. A bacterium, designated W2 was found able to grow in the mining wastewater. 16S rRNA analysis identified the strain as Pseudomonas pseudoalcaligenes which could tolerate up to 39 mg/L cyanide concentration and growth was depleted at 52 mg/L. 60% cyanide degradation was achieved in wastewater containing medium. End-product analysis from high performance liquid chromatography (HPLC) detected formamide implicating the role of cyanide hydratase in cyanide degradation. It can be concluded that P. pseudoalcaligenes is capable of biodegrading cyanide and its potential in wastewater treatment containing cyanide is feasible.
format	Article
author	Belinda Tiong, Zaratulnur Mohd Bahari, Nor Sahslin Irwan Shah Lee, Jafariah Jaafar, Zaharah Ibrahim, Shafinaz Shahir,
spellingShingle	Belinda Tiong, Zaratulnur Mohd Bahari, Nor Sahslin Irwan Shah Lee, Jafariah Jaafar, Zaharah Ibrahim, Shafinaz Shahir, Cyanide degradation by pseudomonas pseudoalcaligenes strain W2 Isolated from mining effluent
author_facet	Belinda Tiong, Zaratulnur Mohd Bahari, Nor Sahslin Irwan Shah Lee, Jafariah Jaafar, Zaharah Ibrahim, Shafinaz Shahir,
author_sort	Belinda Tiong,
title	Cyanide degradation by pseudomonas pseudoalcaligenes strain W2 Isolated from mining effluent
title_short	Cyanide degradation by pseudomonas pseudoalcaligenes strain W2 Isolated from mining effluent
title_full	Cyanide degradation by pseudomonas pseudoalcaligenes strain W2 Isolated from mining effluent
title_fullStr	Cyanide degradation by pseudomonas pseudoalcaligenes strain W2 Isolated from mining effluent
title_full_unstemmed	Cyanide degradation by pseudomonas pseudoalcaligenes strain W2 Isolated from mining effluent
title_sort	cyanide degradation by pseudomonas pseudoalcaligenes strain w2 isolated from mining effluent
publisher	Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/8307/ http://journalarticle.ukm.my/8307/ http://journalarticle.ukm.my/8307/1/10_Belinda_Tiong.pdf
first_indexed	2023-09-18T19:52:03Z
last_indexed	2023-09-18T19:52:03Z
_version_	1777406284626132992

Cyanide degradation by pseudomonas pseudoalcaligenes strain W2 Isolated from mining effluent

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