Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features

Colorectal cancer develops in a multi-step process and is associated with genetic alterations. Blocking apoptosis is a key factor to unlimited cell proliferation and immortalization. Survivin expression inhibits the programmed cell death process, apoptosis. This molecule could play a potential rol...

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Main Authors: Said Saleh, King, Alfred Yin Lam, Yik, Hong Ho
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2014
Online Access:http://journalarticle.ukm.my/8677/
http://journalarticle.ukm.my/8677/
http://journalarticle.ukm.my/8677/1/43_2_05.pdf
id ukm-8677
recordtype eprints
spelling ukm-86772016-12-14T06:47:51Z http://journalarticle.ukm.my/8677/ Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features Said Saleh, King, Alfred Yin Lam Yik, Hong Ho Colorectal cancer develops in a multi-step process and is associated with genetic alterations. Blocking apoptosis is a key factor to unlimited cell proliferation and immortalization. Survivin expression inhibits the programmed cell death process, apoptosis. This molecule could play a potential role in cancer development. Survivin is an attractive target for clinical trials to develop cancer treatment. RNA was prepared from colorectal tumor and adjacent non-tumor tissues and then transcribed to complementary DNA. Human survivin mRNA levels were quantitatively measured by real time polymerase chain reaction (PCR) in tumor and adjacent non-tumor tissues. Expression levels of survivin mRNA in adenocarcinomas were significantly higher than in non-tumor mucosa (p < 0.0001). The expressions of survivin mRNA in adenocarcinomas were related to the degree of differentiation (survivin; p=0.034). No difference was found with other clinicopathological features. These findings indicate survivin role in colorectal carcinogenesis. Survivin could be used as a potential diagnostic and prognostic marker in colorectal cancer. Successful inhibition of this molecule could lead to the development of a new drug for cancer therapy. Penerbit Universiti Kebangsaan Malaysia 2014-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8677/1/43_2_05.pdf Said Saleh, and King, Alfred Yin Lam and Yik, Hong Ho (2014) Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features. Malaysian Applied Biology, 43 (2). pp. 35-40. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=460&catid=59:current-view&Itemid=56
repository_type Digital Repository
institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description Colorectal cancer develops in a multi-step process and is associated with genetic alterations. Blocking apoptosis is a key factor to unlimited cell proliferation and immortalization. Survivin expression inhibits the programmed cell death process, apoptosis. This molecule could play a potential role in cancer development. Survivin is an attractive target for clinical trials to develop cancer treatment. RNA was prepared from colorectal tumor and adjacent non-tumor tissues and then transcribed to complementary DNA. Human survivin mRNA levels were quantitatively measured by real time polymerase chain reaction (PCR) in tumor and adjacent non-tumor tissues. Expression levels of survivin mRNA in adenocarcinomas were significantly higher than in non-tumor mucosa (p < 0.0001). The expressions of survivin mRNA in adenocarcinomas were related to the degree of differentiation (survivin; p=0.034). No difference was found with other clinicopathological features. These findings indicate survivin role in colorectal carcinogenesis. Survivin could be used as a potential diagnostic and prognostic marker in colorectal cancer. Successful inhibition of this molecule could lead to the development of a new drug for cancer therapy.
format Article
author Said Saleh,
King, Alfred Yin Lam
Yik, Hong Ho
spellingShingle Said Saleh,
King, Alfred Yin Lam
Yik, Hong Ho
Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features
author_facet Said Saleh,
King, Alfred Yin Lam
Yik, Hong Ho
author_sort Said Saleh,
title Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features
title_short Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features
title_full Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features
title_fullStr Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features
title_full_unstemmed Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features
title_sort survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2014
url http://journalarticle.ukm.my/8677/
http://journalarticle.ukm.my/8677/
http://journalarticle.ukm.my/8677/1/43_2_05.pdf
first_indexed 2023-09-18T19:52:56Z
last_indexed 2023-09-18T19:52:56Z
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