Analysis of paracetamol in forensic blowfly samples from intoxicated-paracetamol carcass

Paracetamol is one of the most commonly used analgesic drugs that often reported to be abused and involved in intoxication death cases. Some of these cases involved decomposed biological samples from the dead remains, which cannot be analysed appropriately by methods applied for ante mortem specim...

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Main Authors:	Almie Amira Munaras Khan, Wan Mohd Asyraf Wan Mahmood, Siti Aisyah Shamsuddin, Nurul Syahnidz Adila Zaini, Khairulmazidah Mohamed, Rumiza Abd Rashid
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/8703/ http://journalarticle.ukm.my/8703/ http://journalarticle.ukm.my/8703/1/44_1_06.pdf

id	ukm-8703
recordtype	eprints
spelling	ukm-87032016-12-14T06:47:56Z http://journalarticle.ukm.my/8703/ Analysis of paracetamol in forensic blowfly samples from intoxicated-paracetamol carcass Almie Amira Munaras Khan, Wan Mohd Asyraf Wan Mahmood, Siti Aisyah Shamsuddin, Nurul Syahnidz Adila Zaini, Khairulmazidah Mohamed, Rumiza Abd Rashid, Paracetamol is one of the most commonly used analgesic drugs that often reported to be abused and involved in intoxication death cases. Some of these cases involved decomposed biological samples from the dead remains, which cannot be analysed appropriately by methods applied for ante mortem specimens. In this study a method for determining paracetamol in entomological samples obtained from decomposed paracetamol-intoxicated carcass is described. As alternative, entomological samples which is blowflies larvae was used for toxicology analysis since immature blowflies were the main processor for the dead remains. To obtain a paracetamol-exposed carcass, New Zealand white rabbits were ingested with 5600 mg/kg (dose) of paracetamol and were let to decompose. Blowflies larvae consumed the carcasses were collected and paracetamol from the larvae were analysed using High Performance Liquid Chromatography with Diode Array Detector (HPLC-DAD). Paracetamol extraction from blowflies’ larvae was done using Solid Phase Extraction (SPE). Separation was conducted under 75:25 ratios of acidified water (pH=3) and acetonitrile, using a C-18 column. Calibration curve was obtained with linear regression (R2) of 0.9999. Paracetamol peak was eluted out at retention time of 3.7 minutes. The entomological specimen from third instar larvae found out to contain the highest concentration of paracetamol. Penerbit Universiti Kebangsaan Malaysia 2015-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8703/1/44_1_06.pdf Almie Amira Munaras Khan, and Wan Mohd Asyraf Wan Mahmood, and Siti Aisyah Shamsuddin, and Nurul Syahnidz Adila Zaini, and Khairulmazidah Mohamed, and Rumiza Abd Rashid, (2015) Analysis of paracetamol in forensic blowfly samples from intoxicated-paracetamol carcass. Malaysian Applied Biology, 44 (1). pp. 31-35. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=505&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Paracetamol is one of the most commonly used analgesic drugs that often reported to be abused and involved in intoxication death cases. Some of these cases involved decomposed biological samples from the dead remains, which cannot be analysed appropriately by methods applied for ante mortem specimens. In this study a method for determining paracetamol in entomological samples obtained from decomposed paracetamol-intoxicated carcass is described. As alternative, entomological samples which is blowflies larvae was used for toxicology analysis since immature blowflies were the main processor for the dead remains. To obtain a paracetamol-exposed carcass, New Zealand white rabbits were ingested with 5600 mg/kg (dose) of paracetamol and were let to decompose. Blowflies larvae consumed the carcasses were collected and paracetamol from the larvae were analysed using High Performance Liquid Chromatography with Diode Array Detector (HPLC-DAD). Paracetamol extraction from blowflies’ larvae was done using Solid Phase Extraction (SPE). Separation was conducted under 75:25 ratios of acidified water (pH=3) and acetonitrile, using a C-18 column. Calibration curve was obtained with linear regression (R2) of 0.9999. Paracetamol peak was eluted out at retention time of 3.7 minutes. The entomological specimen from third instar larvae found out to contain the highest concentration of paracetamol.
format	Article
author	Almie Amira Munaras Khan, Wan Mohd Asyraf Wan Mahmood, Siti Aisyah Shamsuddin, Nurul Syahnidz Adila Zaini, Khairulmazidah Mohamed, Rumiza Abd Rashid,
spellingShingle	Almie Amira Munaras Khan, Wan Mohd Asyraf Wan Mahmood, Siti Aisyah Shamsuddin, Nurul Syahnidz Adila Zaini, Khairulmazidah Mohamed, Rumiza Abd Rashid, Analysis of paracetamol in forensic blowfly samples from intoxicated-paracetamol carcass
author_facet	Almie Amira Munaras Khan, Wan Mohd Asyraf Wan Mahmood, Siti Aisyah Shamsuddin, Nurul Syahnidz Adila Zaini, Khairulmazidah Mohamed, Rumiza Abd Rashid,
author_sort	Almie Amira Munaras Khan,
title	Analysis of paracetamol in forensic blowfly samples from intoxicated-paracetamol carcass
title_short	Analysis of paracetamol in forensic blowfly samples from intoxicated-paracetamol carcass
title_full	Analysis of paracetamol in forensic blowfly samples from intoxicated-paracetamol carcass
title_fullStr	Analysis of paracetamol in forensic blowfly samples from intoxicated-paracetamol carcass
title_full_unstemmed	Analysis of paracetamol in forensic blowfly samples from intoxicated-paracetamol carcass
title_sort	analysis of paracetamol in forensic blowfly samples from intoxicated-paracetamol carcass
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/8703/ http://journalarticle.ukm.my/8703/ http://journalarticle.ukm.my/8703/1/44_1_06.pdf
first_indexed	2023-09-18T19:53:02Z
last_indexed	2023-09-18T19:53:02Z
_version_	1777406346750066688

Analysis of paracetamol in forensic blowfly samples from intoxicated-paracetamol carcass

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