Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time

Oil Palm Knotted-like 1(OPKN1) gene is a type of homeobox gene that was previously isolated from cDNA library of oil palm’s suspension cell culture. Based on semi-quantitative RT-PCR, northern blotting and in situ hybridization analysis, the OPKN1 gene is active in flower meristem suggesting its i...

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Main Authors:	Mohd Waznul Adly, M.Z., Zuraida, A.B., Wee, C.Y., Alizah, Z., Norliza, A.B., Che Radziah, C.M.Z.
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/8715/ http://journalarticle.ukm.my/8715/ http://journalarticle.ukm.my/8715/1/44_1_17.pdf

id	ukm-8715
recordtype	eprints
spelling	ukm-87152016-12-14T06:47:59Z http://journalarticle.ukm.my/8715/ Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time Mohd Waznul Adly, M.Z. Zuraida, A.B. Wee, C.Y. Alizah, Z. Norliza, A.B. Che Radziah, C.M.Z. Oil Palm Knotted-like 1(OPKN1) gene is a type of homeobox gene that was previously isolated from cDNA library of oil palm’s suspension cell culture. Based on semi-quantitative RT-PCR, northern blotting and in situ hybridization analysis, the OPKN1 gene is active in flower meristem suggesting its involvement in flowering phase. Semi-quantitative RT-PCR analysis indicated that OPKN1 gene is active in both male and female flowers and also in abnormal flower suggesting its role in flower morphogenesis process. To further validate the function of this gene, the overexpression transformation vector of OPKN1 gene driven by CaMV 35S constitutive promoter was constructed. The constructed vector was transformed into plant model systems Nicotiana benthamiana via Agrobacterium tumefaciens. Four transgenics lines of N. benthamiana were successfully obtained and analysis of quantitative Real-Time PCR shows that OPKN1 gene was highly overexpressed in all transgenic lines. Overexpression of OPKN1 gene in N. benthamiana transformant has caused reduction in N. benthamiana’s flower size and flowering time was also observed to be delayed. Based on this finding, it was suggested that OPKN1 may be involved in flower development and morphogenesis process. Penerbit Universiti Kebangsaan Malaysia 2015-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8715/1/44_1_17.pdf Mohd Waznul Adly, M.Z. and Zuraida, A.B. and Wee, C.Y. and Alizah, Z. and Norliza, A.B. and Che Radziah, C.M.Z. (2015) Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time. Malaysian Applied Biology, 44 (1). pp. 101-106. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=505&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Oil Palm Knotted-like 1(OPKN1) gene is a type of homeobox gene that was previously isolated from cDNA library of oil palm’s suspension cell culture. Based on semi-quantitative RT-PCR, northern blotting and in situ hybridization analysis, the OPKN1 gene is active in flower meristem suggesting its involvement in flowering phase. Semi-quantitative RT-PCR analysis indicated that OPKN1 gene is active in both male and female flowers and also in abnormal flower suggesting its role in flower morphogenesis process. To further validate the function of this gene, the overexpression transformation vector of OPKN1 gene driven by CaMV 35S constitutive promoter was constructed. The constructed vector was transformed into plant model systems Nicotiana benthamiana via Agrobacterium tumefaciens. Four transgenics lines of N. benthamiana were successfully obtained and analysis of quantitative Real-Time PCR shows that OPKN1 gene was highly overexpressed in all transgenic lines. Overexpression of OPKN1 gene in N. benthamiana transformant has caused reduction in N. benthamiana’s flower size and flowering time was also observed to be delayed. Based on this finding, it was suggested that OPKN1 may be involved in flower development and morphogenesis process.
format	Article
author	Mohd Waznul Adly, M.Z. Zuraida, A.B. Wee, C.Y. Alizah, Z. Norliza, A.B. Che Radziah, C.M.Z.
spellingShingle	Mohd Waznul Adly, M.Z. Zuraida, A.B. Wee, C.Y. Alizah, Z. Norliza, A.B. Che Radziah, C.M.Z. Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time
author_facet	Mohd Waznul Adly, M.Z. Zuraida, A.B. Wee, C.Y. Alizah, Z. Norliza, A.B. Che Radziah, C.M.Z.
author_sort	Mohd Waznul Adly, M.Z.
title	Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time
title_short	Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time
title_full	Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time
title_fullStr	Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time
title_full_unstemmed	Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time
title_sort	ectopic expression of opkn1 gene in n. benthamiana alter flower size and delay flowering time
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/8715/ http://journalarticle.ukm.my/8715/ http://journalarticle.ukm.my/8715/1/44_1_17.pdf
first_indexed	2023-09-18T19:53:04Z
last_indexed	2023-09-18T19:53:04Z
_version_	1777406348567248896

Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time

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