Paraquat dichloride detection from forensic blowfly samples

Accidental ingestion of paraquat dichloride contributed for a high number of pesticide-related deaths. It is a major suicide agent in many developing countries because of its easy accessibility. Based on the escalated death events related to misuse of paraquat, a study was conducted to observe its...

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Main Authors:	Wan Mohd Asyraf Wan Mahmood, Almie Amira Munaras Khan, Siti Aisyah Shamsuddin, Nurul Syahnidz Adila Zaini, Khairulmazidah Mohamed, Rumiza Abd Rashid
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/8720/ http://journalarticle.ukm.my/8720/ http://journalarticle.ukm.my/8720/1/44_1_22.pdf

id	ukm-8720
recordtype	eprints
spelling	ukm-87202016-12-14T06:48:00Z http://journalarticle.ukm.my/8720/ Paraquat dichloride detection from forensic blowfly samples Wan Mohd Asyraf Wan Mahmood, Almie Amira Munaras Khan, Siti Aisyah Shamsuddin, Nurul Syahnidz Adila Zaini, Khairulmazidah Mohamed, Rumiza Abd Rashid, Accidental ingestion of paraquat dichloride contributed for a high number of pesticide-related deaths. It is a major suicide agent in many developing countries because of its easy accessibility. Based on the escalated death events related to misuse of paraquat, a study was conducted to observe its effect on decomposition process of New Zealand white rabbits, hence, biological and entomological samples were obtained for analysis. Entomological samples especially blowflies which are the main processor of dead remains applied in forensic investigations to obtain time and cause of death. In this research, high performance liquid chromatography (HPLC) was introduced in toxicological analysis to determine the presence of paraquat dichloride from biological specimens, and exploring the potential of blowfly samples as an alternate sample when biological specimen had decomposed. Paraquat dichloride was extracted using C18 solid phase extraction (SPE) to retain the interferences. Mobile phases used were acidified water (pH 3) and acetonitrile with ratio of 85:15. Limit of detection (LOD) and limit of quantitation (LOQ) were 0.03 mg/L and 0.09 mg/L, respectively with a linear curve (R2 = 0.9982). From the analysis, concentration of paraquat dichloride found out to be the highest in liver as well as in the third instar of blowfly larvae. The isolation and analytical method proposed in this present study were able to be applied in detection of paraquat dichloride in post-mortem specimens. In conclusion, blowfly samples were suitable to be applied in forensic toxicological analysis to replace decomposed biological samples. Penerbit Universiti Kebangsaan Malaysia 2015-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8720/1/44_1_22.pdf Wan Mohd Asyraf Wan Mahmood, and Almie Amira Munaras Khan, and Siti Aisyah Shamsuddin, and Nurul Syahnidz Adila Zaini, and Khairulmazidah Mohamed, and Rumiza Abd Rashid, (2015) Paraquat dichloride detection from forensic blowfly samples. Malaysian Applied Biology, 44 (1). pp. 133-138. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=505&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Accidental ingestion of paraquat dichloride contributed for a high number of pesticide-related deaths. It is a major suicide agent in many developing countries because of its easy accessibility. Based on the escalated death events related to misuse of paraquat, a study was conducted to observe its effect on decomposition process of New Zealand white rabbits, hence, biological and entomological samples were obtained for analysis. Entomological samples especially blowflies which are the main processor of dead remains applied in forensic investigations to obtain time and cause of death. In this research, high performance liquid chromatography (HPLC) was introduced in toxicological analysis to determine the presence of paraquat dichloride from biological specimens, and exploring the potential of blowfly samples as an alternate sample when biological specimen had decomposed. Paraquat dichloride was extracted using C18 solid phase extraction (SPE) to retain the interferences. Mobile phases used were acidified water (pH 3) and acetonitrile with ratio of 85:15. Limit of detection (LOD) and limit of quantitation (LOQ) were 0.03 mg/L and 0.09 mg/L, respectively with a linear curve (R2 = 0.9982). From the analysis, concentration of paraquat dichloride found out to be the highest in liver as well as in the third instar of blowfly larvae. The isolation and analytical method proposed in this present study were able to be applied in detection of paraquat dichloride in post-mortem specimens. In conclusion, blowfly samples were suitable to be applied in forensic toxicological analysis to replace decomposed biological samples.
format	Article
author	Wan Mohd Asyraf Wan Mahmood, Almie Amira Munaras Khan, Siti Aisyah Shamsuddin, Nurul Syahnidz Adila Zaini, Khairulmazidah Mohamed, Rumiza Abd Rashid,
spellingShingle	Wan Mohd Asyraf Wan Mahmood, Almie Amira Munaras Khan, Siti Aisyah Shamsuddin, Nurul Syahnidz Adila Zaini, Khairulmazidah Mohamed, Rumiza Abd Rashid, Paraquat dichloride detection from forensic blowfly samples
author_facet	Wan Mohd Asyraf Wan Mahmood, Almie Amira Munaras Khan, Siti Aisyah Shamsuddin, Nurul Syahnidz Adila Zaini, Khairulmazidah Mohamed, Rumiza Abd Rashid,
author_sort	Wan Mohd Asyraf Wan Mahmood,
title	Paraquat dichloride detection from forensic blowfly samples
title_short	Paraquat dichloride detection from forensic blowfly samples
title_full	Paraquat dichloride detection from forensic blowfly samples
title_fullStr	Paraquat dichloride detection from forensic blowfly samples
title_full_unstemmed	Paraquat dichloride detection from forensic blowfly samples
title_sort	paraquat dichloride detection from forensic blowfly samples
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/8720/ http://journalarticle.ukm.my/8720/ http://journalarticle.ukm.my/8720/1/44_1_22.pdf
first_indexed	2023-09-18T19:53:05Z
last_indexed	2023-09-18T19:53:05Z
_version_	1777406349294960640

Paraquat dichloride detection from forensic blowfly samples

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