Effect of low gamma radiation on the growth of Vanilla planifolia cultures

A study was conducted to determine the effect of low doses of gamma radiation on the regeneration and morphological of Vanilla planifolia, a tropical climbing orchid from the family Orchidaceae. The explants were treated with seven different combinations of sterilization agents before inoculating...

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Main Authors:	Ellia Kartini, M., Norrizah, J.S., Noor Anilizawatima, S., Siti Safura, J.
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/8917/ http://journalarticle.ukm.my/8917/ http://journalarticle.ukm.my/8917/1/44_2_05.pdf

id	ukm-8917
recordtype	eprints
spelling	ukm-89172016-12-14T06:48:25Z http://journalarticle.ukm.my/8917/ Effect of low gamma radiation on the growth of Vanilla planifolia cultures Ellia Kartini, M. Norrizah, J.S. Noor Anilizawatima, S. Siti Safura, J. A study was conducted to determine the effect of low doses of gamma radiation on the regeneration and morphological of Vanilla planifolia, a tropical climbing orchid from the family Orchidaceae. The explants were treated with seven different combinations of sterilization agents before inoculating on Murashige and Skoog medium and incubated for three weeks at 25±2ºC. After incubation, the cultures were exposed to three doses of gamma radiation (10 Gy, 20 Gy and 30 Gy). After twelve weeks, the result showed that regeneration of V. planifolia was the highest at 10 Gy irradiation, where shoot height was 13.40±0.33 cm compared to the non-irradiated culture with only 6.89±0.21 cm. The leaf and root numbers as well as root length also showed the highest for 10 Gy cultures. The lowest regeneration was obtained from 30 Gy irradiated cultures. However, no morphological changes were detected in all treated cultures. Thus, it can be suggested that low doses of gamma irradiation stimulate the in vitro regeneration of V. planifolia. Penerbit Universiti Kebangsaan Malaysia 2015-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8917/1/44_2_05.pdf Ellia Kartini, M. and Norrizah, J.S. and Noor Anilizawatima, S. and Siti Safura, J. (2015) Effect of low gamma radiation on the growth of Vanilla planifolia cultures. Malaysian Applied Biology, 44 (2). pp. 59-63. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=519&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	A study was conducted to determine the effect of low doses of gamma radiation on the regeneration and morphological of Vanilla planifolia, a tropical climbing orchid from the family Orchidaceae. The explants were treated with seven different combinations of sterilization agents before inoculating on Murashige and Skoog medium and incubated for three weeks at 25±2ºC. After incubation, the cultures were exposed to three doses of gamma radiation (10 Gy, 20 Gy and 30 Gy). After twelve weeks, the result showed that regeneration of V. planifolia was the highest at 10 Gy irradiation, where shoot height was 13.40±0.33 cm compared to the non-irradiated culture with only 6.89±0.21 cm. The leaf and root numbers as well as root length also showed the highest for 10 Gy cultures. The lowest regeneration was obtained from 30 Gy irradiated cultures. However, no morphological changes were detected in all treated cultures. Thus, it can be suggested that low doses of gamma irradiation stimulate the in vitro regeneration of V. planifolia.
format	Article
author	Ellia Kartini, M. Norrizah, J.S. Noor Anilizawatima, S. Siti Safura, J.
spellingShingle	Ellia Kartini, M. Norrizah, J.S. Noor Anilizawatima, S. Siti Safura, J. Effect of low gamma radiation on the growth of Vanilla planifolia cultures
author_facet	Ellia Kartini, M. Norrizah, J.S. Noor Anilizawatima, S. Siti Safura, J.
author_sort	Ellia Kartini, M.
title	Effect of low gamma radiation on the growth of Vanilla planifolia cultures
title_short	Effect of low gamma radiation on the growth of Vanilla planifolia cultures
title_full	Effect of low gamma radiation on the growth of Vanilla planifolia cultures
title_fullStr	Effect of low gamma radiation on the growth of Vanilla planifolia cultures
title_full_unstemmed	Effect of low gamma radiation on the growth of Vanilla planifolia cultures
title_sort	effect of low gamma radiation on the growth of vanilla planifolia cultures
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/8917/ http://journalarticle.ukm.my/8917/ http://journalarticle.ukm.my/8917/1/44_2_05.pdf
first_indexed	2023-09-18T19:53:30Z
last_indexed	2023-09-18T19:53:30Z
_version_	1777406375455883264

Effect of low gamma radiation on the growth of Vanilla planifolia cultures

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