Molecular characterization of colletotrichum isolates associated with anthracnose of mango fruit

Colletotrichum species are well-known causal agent of anthracnose. A study was conducted to determine the identity of Colletotrichum associated with anthracnose of mango (Mangifera indica) fruits. Thirty five Colletotrichum isolates were isolated from anthracnose lesion of two mango cultivars, Choka...

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Main Authors: Latiffah Zakaria, Nurul Zaadah Juhari, Suzianti Iskandar Vijaya, Intan Sakinah Mohd Anuar
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2015
Online Access:http://journalarticle.ukm.my/8929/
http://journalarticle.ukm.my/8929/
http://journalarticle.ukm.my/8929/1/02_Latiffah_Zakaria.pdf
id ukm-8929
recordtype eprints
spelling ukm-89292016-12-14T06:48:27Z http://journalarticle.ukm.my/8929/ Molecular characterization of colletotrichum isolates associated with anthracnose of mango fruit Latiffah Zakaria, Nurul Zaadah Juhari, Suzianti Iskandar Vijaya, Intan Sakinah Mohd Anuar, Colletotrichum species are well-known causal agent of anthracnose. A study was conducted to determine the identity of Colletotrichum associated with anthracnose of mango (Mangifera indica) fruits. Thirty five Colletotrichum isolates were isolated from anthracnose lesion of two mango cultivars, Chokanan and Harum Manis. Based on the conidial morphology, two morphotypes (I and II) of C. gleosporioides were identified. Based on BLAST search of ITS regions and β-tubulin sequences, majority of the isolates showed 99-100% similarity with Colletotrichum sp. from mango and other hosts and three isolates, 100% similarity with C. asianum. From phylogenetic analysis using maximum likelihood method of combined datasets, the isolates from mango formed three clades, which corresponded to C. gloeosporioides sensu lato and C. asianum. Therefore, the present study showed that the isolates associated with anthracnose of mango belong to C. gleosporioides sensu lato and C. asianum. Universiti Kebangsaan Malaysia 2015-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8929/1/02_Latiffah_Zakaria.pdf Latiffah Zakaria, and Nurul Zaadah Juhari, and Suzianti Iskandar Vijaya, and Intan Sakinah Mohd Anuar, (2015) Molecular characterization of colletotrichum isolates associated with anthracnose of mango fruit. Sains Malaysiana, 44 (5). pp. 651-656. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid44bil5_2015/KandunganJilid44Bil5_2015.html
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description Colletotrichum species are well-known causal agent of anthracnose. A study was conducted to determine the identity of Colletotrichum associated with anthracnose of mango (Mangifera indica) fruits. Thirty five Colletotrichum isolates were isolated from anthracnose lesion of two mango cultivars, Chokanan and Harum Manis. Based on the conidial morphology, two morphotypes (I and II) of C. gleosporioides were identified. Based on BLAST search of ITS regions and β-tubulin sequences, majority of the isolates showed 99-100% similarity with Colletotrichum sp. from mango and other hosts and three isolates, 100% similarity with C. asianum. From phylogenetic analysis using maximum likelihood method of combined datasets, the isolates from mango formed three clades, which corresponded to C. gloeosporioides sensu lato and C. asianum. Therefore, the present study showed that the isolates associated with anthracnose of mango belong to C. gleosporioides sensu lato and C. asianum.
format Article
author Latiffah Zakaria,
Nurul Zaadah Juhari,
Suzianti Iskandar Vijaya,
Intan Sakinah Mohd Anuar,
spellingShingle Latiffah Zakaria,
Nurul Zaadah Juhari,
Suzianti Iskandar Vijaya,
Intan Sakinah Mohd Anuar,
Molecular characterization of colletotrichum isolates associated with anthracnose of mango fruit
author_facet Latiffah Zakaria,
Nurul Zaadah Juhari,
Suzianti Iskandar Vijaya,
Intan Sakinah Mohd Anuar,
author_sort Latiffah Zakaria,
title Molecular characterization of colletotrichum isolates associated with anthracnose of mango fruit
title_short Molecular characterization of colletotrichum isolates associated with anthracnose of mango fruit
title_full Molecular characterization of colletotrichum isolates associated with anthracnose of mango fruit
title_fullStr Molecular characterization of colletotrichum isolates associated with anthracnose of mango fruit
title_full_unstemmed Molecular characterization of colletotrichum isolates associated with anthracnose of mango fruit
title_sort molecular characterization of colletotrichum isolates associated with anthracnose of mango fruit
publisher Universiti Kebangsaan Malaysia
publishDate 2015
url http://journalarticle.ukm.my/8929/
http://journalarticle.ukm.my/8929/
http://journalarticle.ukm.my/8929/1/02_Latiffah_Zakaria.pdf
first_indexed 2023-09-18T19:53:31Z
last_indexed 2023-09-18T19:53:31Z
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