New lipase producing β-proteobacteria Strains Caldimonas sp. and Tepidimonas sp. isolated from a Malaysian Hot Springs

Microbial lipolytic enzymes have attracted considerable attention owing to their biotechnological potential. In this study, thermophilic bacteria producing lipase were isolated from Bentong and Sungai Lembing hot springs, in Pahang, Malaysia. Out of 25 colonies isolated, 14 samples showed to produce...

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Main Authors:	Sharul Azim Mohd Ghazali, Tengku Haziyamin Tengku Abdul Hamid
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/8945/ http://journalarticle.ukm.my/8945/ http://journalarticle.ukm.my/8945/1/09_Sharul_Azim_Mohd_Ghazali.pdf

id	ukm-8945
recordtype	eprints
spelling	ukm-89452016-12-14T06:48:31Z http://journalarticle.ukm.my/8945/ New lipase producing β-proteobacteria Strains Caldimonas sp. and Tepidimonas sp. isolated from a Malaysian Hot Springs Sharul Azim Mohd Ghazali, Tengku Haziyamin Tengku Abdul Hamid, Microbial lipolytic enzymes have attracted considerable attention owing to their biotechnological potential. In this study, thermophilic bacteria producing lipase were isolated from Bentong and Sungai Lembing hot springs, in Pahang, Malaysia. Out of 25 colonies isolated, 14 samples showed to produce clear zones surrounding the growth on tributyrin and trioelin agar plates. All 14 isolates showed Gram-negative bacteria with short rod morphology. PCR amplification of 16S ribosomal DNA gene showed that these isolates were clustered with subclass β-proteobacteria consisting of thermophilic bacteria that produce lipase. Phylogenetic analysis was carried out with the highly similar species and 4 isolates (SglA1, BtnC1, BtnC2 and BtnC3) are related to genus Caldimonas and 10 isolates (SglB1, SglB2, SglB3, SglB4, BtnB1, BtnB2 BtnD1, BtnD2, BtnD3 and BtnD4) belonged to genus Tepidimonas. These results indicated that novel lipase-producing thermophilic β-proteobacteria could be isolated from these hot springs. Universiti Kebangsaan Malaysia 2015-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8945/1/09_Sharul_Azim_Mohd_Ghazali.pdf Sharul Azim Mohd Ghazali, and Tengku Haziyamin Tengku Abdul Hamid, (2015) New lipase producing β-proteobacteria Strains Caldimonas sp. and Tepidimonas sp. isolated from a Malaysian Hot Springs. Sains Malaysiana, 44 (5). pp. 701-706. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid44bil5_2015/KandunganJilid44Bil5_2015.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Microbial lipolytic enzymes have attracted considerable attention owing to their biotechnological potential. In this study, thermophilic bacteria producing lipase were isolated from Bentong and Sungai Lembing hot springs, in Pahang, Malaysia. Out of 25 colonies isolated, 14 samples showed to produce clear zones surrounding the growth on tributyrin and trioelin agar plates. All 14 isolates showed Gram-negative bacteria with short rod morphology. PCR amplification of 16S ribosomal DNA gene showed that these isolates were clustered with subclass β-proteobacteria consisting of thermophilic bacteria that produce lipase. Phylogenetic analysis was carried out with the highly similar species and 4 isolates (SglA1, BtnC1, BtnC2 and BtnC3) are related to genus Caldimonas and 10 isolates (SglB1, SglB2, SglB3, SglB4, BtnB1, BtnB2 BtnD1, BtnD2, BtnD3 and BtnD4) belonged to genus Tepidimonas. These results indicated that novel lipase-producing thermophilic β-proteobacteria could be isolated from these hot springs.
format	Article
author	Sharul Azim Mohd Ghazali, Tengku Haziyamin Tengku Abdul Hamid,
spellingShingle	Sharul Azim Mohd Ghazali, Tengku Haziyamin Tengku Abdul Hamid, New lipase producing β-proteobacteria Strains Caldimonas sp. and Tepidimonas sp. isolated from a Malaysian Hot Springs
author_facet	Sharul Azim Mohd Ghazali, Tengku Haziyamin Tengku Abdul Hamid,
author_sort	Sharul Azim Mohd Ghazali,
title	New lipase producing β-proteobacteria Strains Caldimonas sp. and Tepidimonas sp. isolated from a Malaysian Hot Springs
title_short	New lipase producing β-proteobacteria Strains Caldimonas sp. and Tepidimonas sp. isolated from a Malaysian Hot Springs
title_full	New lipase producing β-proteobacteria Strains Caldimonas sp. and Tepidimonas sp. isolated from a Malaysian Hot Springs
title_fullStr	New lipase producing β-proteobacteria Strains Caldimonas sp. and Tepidimonas sp. isolated from a Malaysian Hot Springs
title_full_unstemmed	New lipase producing β-proteobacteria Strains Caldimonas sp. and Tepidimonas sp. isolated from a Malaysian Hot Springs
title_sort	new lipase producing β-proteobacteria strains caldimonas sp. and tepidimonas sp. isolated from a malaysian hot springs
publisher	Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/8945/ http://journalarticle.ukm.my/8945/ http://journalarticle.ukm.my/8945/1/09_Sharul_Azim_Mohd_Ghazali.pdf
first_indexed	2023-09-18T19:53:33Z
last_indexed	2023-09-18T19:53:33Z
_version_	1777406379402723328

New lipase producing β-proteobacteria Strains Caldimonas sp. and Tepidimonas sp. isolated from a Malaysian Hot Springs

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