Thidiazuron induces high frequency direct somatic embryogenesis growth from cotyledon culture of eurycoma longifolia

Eurycoma longifolia is one of the well-known herbal plants in Southeast Asian region due to its remarkable properties, especially the root part that can be used as aphrodisiac, anti-cancer, anti-malaria and anti-ulcer agent. Uncontrolled harvesting of this plant has reduced its population. Thus, an...

Full description

Bibliographic Details
Main Authors:	Zawawi Dhiya Dalila, Ja’afar Hafsah, Zainuddin Rokiah, Kari Rodziah, Mohd Noor Madihah
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/8974/ http://journalarticle.ukm.my/8974/ http://journalarticle.ukm.my/8974/1/01_Zawawi_Dhiya.pdf

id	ukm-8974
recordtype	eprints
spelling	ukm-89742016-12-14T06:48:36Z http://journalarticle.ukm.my/8974/ Thidiazuron induces high frequency direct somatic embryogenesis growth from cotyledon culture of eurycoma longifolia Zawawi Dhiya Dalila, Ja’afar Hafsah, Zainuddin Rokiah, Kari Rodziah, Mohd Noor Madihah, Eurycoma longifolia is one of the well-known herbal plants in Southeast Asian region due to its remarkable properties, especially the root part that can be used as aphrodisiac, anti-cancer, anti-malaria and anti-ulcer agent. Uncontrolled harvesting of this plant has reduced its population. Thus, an efficient protocol involving the induction of direct somatic embryogenesis from cotyledon culture of E. longifolia has been developed. 15% explants forming embryos were achieved on Modified Murashige and Skoog’s (MMS) medium supplemented with 0.1 mg/L zeatin plus 0.2 mg/L indole-3-butyric acid (IBA). However, the addition of 0.12 mg/L thidiazuron (TDZ) has increased the percentage up to 22.0%. The maturation phase occurred in the same medium, thus decreased the frequency of subculture and the genetic instability of the embryos. Secondary somatic embryogenesis growth of 20%, which was the highest percentage of growth, was achieved from the primary embryos that were cultured in the MMS medium with the treatment of 2.0 mg/L IBA plus 0.075 mg/L TDZ. The results indicated that the direct somatic embryogenesis obtained can be further used to develop a protocol for plantlet regeneration of E. longifolia. Universiti Kebangsaan Malaysia 2015-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8974/1/01_Zawawi_Dhiya.pdf Zawawi Dhiya Dalila, and Ja’afar Hafsah, and Zainuddin Rokiah, and Kari Rodziah, and Mohd Noor Madihah, (2015) Thidiazuron induces high frequency direct somatic embryogenesis growth from cotyledon culture of eurycoma longifolia. Sains Malaysiana, 44 (7). pp. 913-920. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid44bil7_2015/KandunganJilid44Bil7_2015.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Eurycoma longifolia is one of the well-known herbal plants in Southeast Asian region due to its remarkable properties, especially the root part that can be used as aphrodisiac, anti-cancer, anti-malaria and anti-ulcer agent. Uncontrolled harvesting of this plant has reduced its population. Thus, an efficient protocol involving the induction of direct somatic embryogenesis from cotyledon culture of E. longifolia has been developed. 15% explants forming embryos were achieved on Modified Murashige and Skoog’s (MMS) medium supplemented with 0.1 mg/L zeatin plus 0.2 mg/L indole-3-butyric acid (IBA). However, the addition of 0.12 mg/L thidiazuron (TDZ) has increased the percentage up to 22.0%. The maturation phase occurred in the same medium, thus decreased the frequency of subculture and the genetic instability of the embryos. Secondary somatic embryogenesis growth of 20%, which was the highest percentage of growth, was achieved from the primary embryos that were cultured in the MMS medium with the treatment of 2.0 mg/L IBA plus 0.075 mg/L TDZ. The results indicated that the direct somatic embryogenesis obtained can be further used to develop a protocol for plantlet regeneration of E. longifolia.
format	Article
author	Zawawi Dhiya Dalila, Ja’afar Hafsah, Zainuddin Rokiah, Kari Rodziah, Mohd Noor Madihah,
spellingShingle	Zawawi Dhiya Dalila, Ja’afar Hafsah, Zainuddin Rokiah, Kari Rodziah, Mohd Noor Madihah, Thidiazuron induces high frequency direct somatic embryogenesis growth from cotyledon culture of eurycoma longifolia
author_facet	Zawawi Dhiya Dalila, Ja’afar Hafsah, Zainuddin Rokiah, Kari Rodziah, Mohd Noor Madihah,
author_sort	Zawawi Dhiya Dalila,
title	Thidiazuron induces high frequency direct somatic embryogenesis growth from cotyledon culture of eurycoma longifolia
title_short	Thidiazuron induces high frequency direct somatic embryogenesis growth from cotyledon culture of eurycoma longifolia
title_full	Thidiazuron induces high frequency direct somatic embryogenesis growth from cotyledon culture of eurycoma longifolia
title_fullStr	Thidiazuron induces high frequency direct somatic embryogenesis growth from cotyledon culture of eurycoma longifolia
title_full_unstemmed	Thidiazuron induces high frequency direct somatic embryogenesis growth from cotyledon culture of eurycoma longifolia
title_sort	thidiazuron induces high frequency direct somatic embryogenesis growth from cotyledon culture of eurycoma longifolia
publisher	Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/8974/ http://journalarticle.ukm.my/8974/ http://journalarticle.ukm.my/8974/1/01_Zawawi_Dhiya.pdf
first_indexed	2023-09-18T19:53:37Z
last_indexed	2023-09-18T19:53:37Z
_version_	1777406383666233344

Thidiazuron induces high frequency direct somatic embryogenesis growth from cotyledon culture of eurycoma longifolia

Similar Items