Identification and Characterization of Lactic Acid Bacteria (LAB) Isolated from Probiotic Drinks in Malaysia

Many studies have shown that probiotic strains added to a number of probiotic products are not compatible to that of claimed. It is thus of note to validate probiotic strains added to probiotic products. In this study, three probiotic drinks, A, B and C, were cultured on MRS agar and the number of...

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Main Authors:	Wei, Boon Yap, Rina Anak Sujang, Toong, Seng Tan
Format:	Article
Language:	English
Published:	Penerbit UKM 2015
Subjects:	RA Public aspects of medicine
Online Access:	http://journalarticle.ukm.my/9011/ http://journalarticle.ukm.my/9011/ http://journalarticle.ukm.my/9011/1/9466-25517-1-SM.pdf

id	ukm-9011
recordtype	eprints
spelling	ukm-90112016-12-14T06:48:43Z http://journalarticle.ukm.my/9011/ Identification and Characterization of Lactic Acid Bacteria (LAB) Isolated from Probiotic Drinks in Malaysia Wei, Boon Yap Rina Anak Sujang, Toong, Seng Tan RA Public aspects of medicine Many studies have shown that probiotic strains added to a number of probiotic products are not compatible to that of claimed. It is thus of note to validate probiotic strains added to probiotic products. In this study, three probiotic drinks, A, B and C, were cultured on MRS agar and the number of bacterial colonies was enumerated. The bacterial counts recovered from A (9.3 ± 6.9 log CFU/ml) and C (9.0 ± 6.9 log CFU/ml) were signifi cantly higher than B (5.2 ± 3.5 log CFU/ml) and achieved the minimal amount recommended for probiotic bacteria. All of the isolates appeared as gram positive rods microscopically and were proven to be catalase negative. However, there were only A1, A2, B4 and C1 that were highly tolerant to the gastrointestinal pH 3 to 6. The four isolates produced and secreted antimicrobial substances which inhibited the growth of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). C1 showed the greatest growth inhibition by forming 17.50-mm and 17.85-mm inhibition zones against E. coli and S. aureus, respectively. The 16s rDNA sequencing and phylogenetic analysis were performed to further identify the twelve isolates. The twelve isolates were found to be Lactobacillus (L.), particularly L. casei and L. paracasei. However, the bacteria isolated from drink B were incompatible to the labelled ones. In conclusion, probiotic drinks are possible to contain different bacterial counts and probiotic strains from the labelled ones. These differences might affect health benefi ts rendered by probiotic strains to consumers. Penerbit UKM 2015 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9011/1/9466-25517-1-SM.pdf Wei, Boon Yap and Rina Anak Sujang, and Toong, Seng Tan (2015) Identification and Characterization of Lactic Acid Bacteria (LAB) Isolated from Probiotic Drinks in Malaysia. Jurnal Sains Kesihatan Malaysia, 13 (1). pp. 23-31. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/588
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
topic	RA Public aspects of medicine
spellingShingle	RA Public aspects of medicine Wei, Boon Yap Rina Anak Sujang, Toong, Seng Tan Identification and Characterization of Lactic Acid Bacteria (LAB) Isolated from Probiotic Drinks in Malaysia
description	Many studies have shown that probiotic strains added to a number of probiotic products are not compatible to that of claimed. It is thus of note to validate probiotic strains added to probiotic products. In this study, three probiotic drinks, A, B and C, were cultured on MRS agar and the number of bacterial colonies was enumerated. The bacterial counts recovered from A (9.3 ± 6.9 log CFU/ml) and C (9.0 ± 6.9 log CFU/ml) were signifi cantly higher than B (5.2 ± 3.5 log CFU/ml) and achieved the minimal amount recommended for probiotic bacteria. All of the isolates appeared as gram positive rods microscopically and were proven to be catalase negative. However, there were only A1, A2, B4 and C1 that were highly tolerant to the gastrointestinal pH 3 to 6. The four isolates produced and secreted antimicrobial substances which inhibited the growth of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). C1 showed the greatest growth inhibition by forming 17.50-mm and 17.85-mm inhibition zones against E. coli and S. aureus, respectively. The 16s rDNA sequencing and phylogenetic analysis were performed to further identify the twelve isolates. The twelve isolates were found to be Lactobacillus (L.), particularly L. casei and L. paracasei. However, the bacteria isolated from drink B were incompatible to the labelled ones. In conclusion, probiotic drinks are possible to contain different bacterial counts and probiotic strains from the labelled ones. These differences might affect health benefi ts rendered by probiotic strains to consumers.
format	Article
author	Wei, Boon Yap Rina Anak Sujang, Toong, Seng Tan
author_facet	Wei, Boon Yap Rina Anak Sujang, Toong, Seng Tan
author_sort	Wei, Boon Yap
title	Identification and Characterization of Lactic Acid Bacteria (LAB) Isolated from Probiotic Drinks in Malaysia
title_short	Identification and Characterization of Lactic Acid Bacteria (LAB) Isolated from Probiotic Drinks in Malaysia
title_full	Identification and Characterization of Lactic Acid Bacteria (LAB) Isolated from Probiotic Drinks in Malaysia
title_fullStr	Identification and Characterization of Lactic Acid Bacteria (LAB) Isolated from Probiotic Drinks in Malaysia
title_full_unstemmed	Identification and Characterization of Lactic Acid Bacteria (LAB) Isolated from Probiotic Drinks in Malaysia
title_sort	identification and characterization of lactic acid bacteria (lab) isolated from probiotic drinks in malaysia
publisher	Penerbit UKM
publishDate	2015
url	http://journalarticle.ukm.my/9011/ http://journalarticle.ukm.my/9011/ http://journalarticle.ukm.my/9011/1/9466-25517-1-SM.pdf
first_indexed	2023-09-18T19:53:45Z
last_indexed	2023-09-18T19:53:45Z
_version_	1777406391588225024

Identification and Characterization of Lactic Acid Bacteria (LAB) Isolated from Probiotic Drinks in Malaysia

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