Molecular characterization of Malaysian marine fish species using partial sequence of Mitochondrial DNA 12S and 16S rRNA Markers

This study was conducted to characterize the selected marine fish species using partial sequence of mtDNA 12S and 16S rRNA gene. PCR amplification of 12S and 16S rRNA generated PCR amplicons at 350 and 440 bp lengths, respectively. Sequence analysis was performed using BioEdit software. Phylogenetic...

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Main Authors:	Siti Farah Quraishia, Sundararajulu Panneerchelvam, Zafarina Zainuddin, Nur Haslindawaty Abd Rashid
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/9040/ http://journalarticle.ukm.my/9040/ http://journalarticle.ukm.my/9040/1/06_Siti_Farah.pdf

id	ukm-9040
recordtype	eprints
spelling	ukm-90402016-12-14T06:48:48Z http://journalarticle.ukm.my/9040/ Molecular characterization of Malaysian marine fish species using partial sequence of Mitochondrial DNA 12S and 16S rRNA Markers Siti Farah Quraishia, Sundararajulu Panneerchelvam, Zafarina Zainuddin, Nur Haslindawaty Abd Rashid, This study was conducted to characterize the selected marine fish species using partial sequence of mtDNA 12S and 16S rRNA gene. PCR amplification of 12S and 16S rRNA generated PCR amplicons at 350 and 440 bp lengths, respectively. Sequence analysis was performed using BioEdit software. Phylogenetic tree was constructed using MEGA software. Two reference species have been used namely Gobio gobio (AB239596 and EF112528) and Pentapodus caninus (DQ533268 and DQ532933). Based on the result obtained, mtDNA 12S and 16S rRNA were found to be useful as molecular markers for fish species identification. These markers will provide correct identification of fish species when considered along with morphological characteristics. Universiti Kebangsaan Malaysia 2015-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9040/1/06_Siti_Farah.pdf Siti Farah Quraishia, and Sundararajulu Panneerchelvam, and Zafarina Zainuddin, and Nur Haslindawaty Abd Rashid, (2015) Molecular characterization of Malaysian marine fish species using partial sequence of Mitochondrial DNA 12S and 16S rRNA Markers. Sains Malaysiana, 44 (4). pp. 1119-1123. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol44num8_2015/contentsVol44num8_2015.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	This study was conducted to characterize the selected marine fish species using partial sequence of mtDNA 12S and 16S rRNA gene. PCR amplification of 12S and 16S rRNA generated PCR amplicons at 350 and 440 bp lengths, respectively. Sequence analysis was performed using BioEdit software. Phylogenetic tree was constructed using MEGA software. Two reference species have been used namely Gobio gobio (AB239596 and EF112528) and Pentapodus caninus (DQ533268 and DQ532933). Based on the result obtained, mtDNA 12S and 16S rRNA were found to be useful as molecular markers for fish species identification. These markers will provide correct identification of fish species when considered along with morphological characteristics.
format	Article
author	Siti Farah Quraishia, Sundararajulu Panneerchelvam, Zafarina Zainuddin, Nur Haslindawaty Abd Rashid,
spellingShingle	Siti Farah Quraishia, Sundararajulu Panneerchelvam, Zafarina Zainuddin, Nur Haslindawaty Abd Rashid, Molecular characterization of Malaysian marine fish species using partial sequence of Mitochondrial DNA 12S and 16S rRNA Markers
author_facet	Siti Farah Quraishia, Sundararajulu Panneerchelvam, Zafarina Zainuddin, Nur Haslindawaty Abd Rashid,
author_sort	Siti Farah Quraishia,
title	Molecular characterization of Malaysian marine fish species using partial sequence of Mitochondrial DNA 12S and 16S rRNA Markers
title_short	Molecular characterization of Malaysian marine fish species using partial sequence of Mitochondrial DNA 12S and 16S rRNA Markers
title_full	Molecular characterization of Malaysian marine fish species using partial sequence of Mitochondrial DNA 12S and 16S rRNA Markers
title_fullStr	Molecular characterization of Malaysian marine fish species using partial sequence of Mitochondrial DNA 12S and 16S rRNA Markers
title_full_unstemmed	Molecular characterization of Malaysian marine fish species using partial sequence of Mitochondrial DNA 12S and 16S rRNA Markers
title_sort	molecular characterization of malaysian marine fish species using partial sequence of mitochondrial dna 12s and 16s rrna markers
publisher	Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/9040/ http://journalarticle.ukm.my/9040/ http://journalarticle.ukm.my/9040/1/06_Siti_Farah.pdf
first_indexed	2023-09-18T19:53:49Z
last_indexed	2023-09-18T19:53:49Z
_version_	1777406395908358144

Molecular characterization of Malaysian marine fish species using partial sequence of Mitochondrial DNA 12S and 16S rRNA Markers

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