Supplementation of Psidium guajava (Guava) fruit polysaccharide attenuates paracetamol-induced liver injury by enhancing the endogenous antioxidant activity

This study was aimed to determine the effect of polysaccharide from guava fruit on paracetamol (PCM)-induced liver injury. Aqueous extract of Psidium guajava fruit was treated with 95% ethanol to collect the water soluble polysaccharide precipitates. Thirty-eight male Sprague Dawley rats were divide...

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Main Authors:	Aspalilah Alias, Faizah Othman, Abdul Rashid Li, Aqilah Kamaruddin, Rafizul Yusof, Farida Hussan
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/9042/ http://journalarticle.ukm.my/9042/ http://journalarticle.ukm.my/9042/1/08_Aspalilah_Alias.pdf

id	ukm-9042
recordtype	eprints
spelling	ukm-90422016-12-14T06:48:48Z http://journalarticle.ukm.my/9042/ Supplementation of Psidium guajava (Guava) fruit polysaccharide attenuates paracetamol-induced liver injury by enhancing the endogenous antioxidant activity Aspalilah Alias, Faizah Othman, Abdul Rashid Li, Aqilah Kamaruddin, Rafizul Yusof, Farida Hussan, This study was aimed to determine the effect of polysaccharide from guava fruit on paracetamol (PCM)-induced liver injury. Aqueous extract of Psidium guajava fruit was treated with 95% ethanol to collect the water soluble polysaccharide precipitates. Thirty-eight male Sprague Dawley rats were divided into control (C), PPG400, PCM, PPG200+PCM and PPG400+PCM. The control and PCM groups received 0.9% normal saline orally while the rest were given 200 and 400 mg/kg of freeze-dried polysaccharide (PPG) per oral for fourteen days. At day 15, the animals were orally received PCM (2 g/kg) except the control and PPG400 groups which received 5% dimethyl sulfoxide. At day 16, the blood was collected to determine serum liver enzymes such as transaminases (AST and ALT). The liver tissue was harvested for determination of superoxide dismutase (SOD), glutathione (GSH), tumour necrosis factor-α (TNF-α), interleukin-6(IL6), microscopic changes and glycogen content. The PCM group showed significant higher level of AST, ALT, TNF-α and IL6 than those of group C. The PCM group showed glycogen depletion, vacuolisation, loss of cell membrane, inflammatory cells infiltration and distorted hepatocelluar cords and narrow sinusoidal spaces. However, those PCM-induced alterations were attenuated by the PPG supplementation. Therefore, the polysaccharide of Psidium guajava possesses hepatoprotective activity and can be used as a dietary supplementation for protection of liver. Universiti Kebangsaan Malaysia 2015-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9042/1/08_Aspalilah_Alias.pdf Aspalilah Alias, and Faizah Othman, and Abdul Rashid Li, and Aqilah Kamaruddin, and Rafizul Yusof, and Farida Hussan, (2015) Supplementation of Psidium guajava (Guava) fruit polysaccharide attenuates paracetamol-induced liver injury by enhancing the endogenous antioxidant activity. Sains Malaysiana, 44 (4). pp. 1129-1136. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol44num8_2015/contentsVol44num8_2015.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	This study was aimed to determine the effect of polysaccharide from guava fruit on paracetamol (PCM)-induced liver injury. Aqueous extract of Psidium guajava fruit was treated with 95% ethanol to collect the water soluble polysaccharide precipitates. Thirty-eight male Sprague Dawley rats were divided into control (C), PPG400, PCM, PPG200+PCM and PPG400+PCM. The control and PCM groups received 0.9% normal saline orally while the rest were given 200 and 400 mg/kg of freeze-dried polysaccharide (PPG) per oral for fourteen days. At day 15, the animals were orally received PCM (2 g/kg) except the control and PPG400 groups which received 5% dimethyl sulfoxide. At day 16, the blood was collected to determine serum liver enzymes such as transaminases (AST and ALT). The liver tissue was harvested for determination of superoxide dismutase (SOD), glutathione (GSH), tumour necrosis factor-α (TNF-α), interleukin-6(IL6), microscopic changes and glycogen content. The PCM group showed significant higher level of AST, ALT, TNF-α and IL6 than those of group C. The PCM group showed glycogen depletion, vacuolisation, loss of cell membrane, inflammatory cells infiltration and distorted hepatocelluar cords and narrow sinusoidal spaces. However, those PCM-induced alterations were attenuated by the PPG supplementation. Therefore, the polysaccharide of Psidium guajava possesses hepatoprotective activity and can be used as a dietary supplementation for protection of liver.
format	Article
author	Aspalilah Alias, Faizah Othman, Abdul Rashid Li, Aqilah Kamaruddin, Rafizul Yusof, Farida Hussan,
spellingShingle	Aspalilah Alias, Faizah Othman, Abdul Rashid Li, Aqilah Kamaruddin, Rafizul Yusof, Farida Hussan, Supplementation of Psidium guajava (Guava) fruit polysaccharide attenuates paracetamol-induced liver injury by enhancing the endogenous antioxidant activity
author_facet	Aspalilah Alias, Faizah Othman, Abdul Rashid Li, Aqilah Kamaruddin, Rafizul Yusof, Farida Hussan,
author_sort	Aspalilah Alias,
title	Supplementation of Psidium guajava (Guava) fruit polysaccharide attenuates paracetamol-induced liver injury by enhancing the endogenous antioxidant activity
title_short	Supplementation of Psidium guajava (Guava) fruit polysaccharide attenuates paracetamol-induced liver injury by enhancing the endogenous antioxidant activity
title_full	Supplementation of Psidium guajava (Guava) fruit polysaccharide attenuates paracetamol-induced liver injury by enhancing the endogenous antioxidant activity
title_fullStr	Supplementation of Psidium guajava (Guava) fruit polysaccharide attenuates paracetamol-induced liver injury by enhancing the endogenous antioxidant activity
title_full_unstemmed	Supplementation of Psidium guajava (Guava) fruit polysaccharide attenuates paracetamol-induced liver injury by enhancing the endogenous antioxidant activity
title_sort	supplementation of psidium guajava (guava) fruit polysaccharide attenuates paracetamol-induced liver injury by enhancing the endogenous antioxidant activity
publisher	Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/9042/ http://journalarticle.ukm.my/9042/ http://journalarticle.ukm.my/9042/1/08_Aspalilah_Alias.pdf
first_indexed	2023-09-18T19:53:49Z
last_indexed	2023-09-18T19:53:49Z
_version_	1777406396201959424

Supplementation of Psidium guajava (Guava) fruit polysaccharide attenuates paracetamol-induced liver injury by enhancing the endogenous antioxidant activity

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