Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities

The study aimed to determine the influence of sample preparation and extraction parameters on the antibacterial activity of inflorescences from banana (Musa balbisiaba cv. Saba). Banana inflorescences were extracted using various solvent extractions and tested for antibacterial activity using agar-w...

Full description

Bibliographic Details
Main Authors:	Hoe, Seng Tin, Birdie Scott Padam, Charles S. Vairappan, Mohd Ismail Abdullah, Fook, Yee Chye
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/9265/ http://journalarticle.ukm.my/9265/ http://journalarticle.ukm.my/9265/1/12_Hoe_Seng_Tin.pdf

id	ukm-9265
recordtype	eprints
spelling	ukm-92652016-12-14T06:49:24Z http://journalarticle.ukm.my/9265/ Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities Hoe, Seng Tin Birdie Scott Padam, Charles S. Vairappan, Mohd Ismail Abdullah, Fook, Yee Chye The study aimed to determine the influence of sample preparation and extraction parameters on the antibacterial activity of inflorescences from banana (Musa balbisiaba cv. Saba). Banana inflorescences were extracted using various solvent extractions and tested for antibacterial activity using agar-well diffusion assay against gram-positive bacteria (Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes and Brochothrix thermosphacta) and gram-negative bacteria (Salmonella typhimurium, Salmonella enteritidis, Escherichia coli O157:H7, Enterobacter sakazakii, Yersinia enterocolitica and Vibrio parahaemolyticus) The effects of geographical origin, drying methods and extraction parameters (sample-to-solvent ratio, extraction time and temperature as well as methanol to solvent ratio) on antibacterial activity of the banana by-product were carried out. Among all the extracts evaluated, methanolic extract from the buds showed significant higher inhibitory against all gram positive bacteria ranging from 12.56-13.54 mm. Interestingly, no significant different (p>0.05) was observed on the effect of geographical origin as well as extraction methods on the antibacterial capacity. Meanwhile, the extracts produced from 50°C oven dried sample seem to have comparable antibacterial activity with the freeze dried samples. Extraction parameters (sample-to-solvent ratio, extraction time and temperature as well as methanol to solvent ratio) were found responsible in determining the efficacy of the antibacterial. In conclusion, methanolic extracts from banana inflorescence buds could be a new source of natural antibacterial and further bioassay guided fractionation should be carried out to determine the bioactive compounds and their biological activities. Universiti Kebangsaan Malaysia 2015-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9265/1/12_Hoe_Seng_Tin.pdf Hoe, Seng Tin and Birdie Scott Padam, and Charles S. Vairappan, and Mohd Ismail Abdullah, and Fook, Yee Chye (2015) Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities. Sains Malaysiana, 44 (9). pp. 1301-1307. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid44bil9_2015/KandunganJilid44Bil9_2015.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The study aimed to determine the influence of sample preparation and extraction parameters on the antibacterial activity of inflorescences from banana (Musa balbisiaba cv. Saba). Banana inflorescences were extracted using various solvent extractions and tested for antibacterial activity using agar-well diffusion assay against gram-positive bacteria (Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes and Brochothrix thermosphacta) and gram-negative bacteria (Salmonella typhimurium, Salmonella enteritidis, Escherichia coli O157:H7, Enterobacter sakazakii, Yersinia enterocolitica and Vibrio parahaemolyticus) The effects of geographical origin, drying methods and extraction parameters (sample-to-solvent ratio, extraction time and temperature as well as methanol to solvent ratio) on antibacterial activity of the banana by-product were carried out. Among all the extracts evaluated, methanolic extract from the buds showed significant higher inhibitory against all gram positive bacteria ranging from 12.56-13.54 mm. Interestingly, no significant different (p>0.05) was observed on the effect of geographical origin as well as extraction methods on the antibacterial capacity. Meanwhile, the extracts produced from 50°C oven dried sample seem to have comparable antibacterial activity with the freeze dried samples. Extraction parameters (sample-to-solvent ratio, extraction time and temperature as well as methanol to solvent ratio) were found responsible in determining the efficacy of the antibacterial. In conclusion, methanolic extracts from banana inflorescence buds could be a new source of natural antibacterial and further bioassay guided fractionation should be carried out to determine the bioactive compounds and their biological activities.
format	Article
author	Hoe, Seng Tin Birdie Scott Padam, Charles S. Vairappan, Mohd Ismail Abdullah, Fook, Yee Chye
spellingShingle	Hoe, Seng Tin Birdie Scott Padam, Charles S. Vairappan, Mohd Ismail Abdullah, Fook, Yee Chye Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities
author_facet	Hoe, Seng Tin Birdie Scott Padam, Charles S. Vairappan, Mohd Ismail Abdullah, Fook, Yee Chye
author_sort	Hoe, Seng Tin
title	Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities
title_short	Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities
title_full	Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities
title_fullStr	Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities
title_full_unstemmed	Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities
title_sort	effect of preparation and extraction parameters of banana (musa balbisiana cv. saba) inflorescence on their antibacterial activities
publisher	Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/9265/ http://journalarticle.ukm.my/9265/ http://journalarticle.ukm.my/9265/1/12_Hoe_Seng_Tin.pdf
first_indexed	2023-09-18T19:54:24Z
last_indexed	2023-09-18T19:54:24Z
_version_	1777406432299188224

Effect of preparation and extraction parameters of Banana (Musa balbisiana cv. Saba) inflorescence on their antibacterial activities

Similar Items