Pore Interconnectivity Analysis of Porous Three Dimensional Scaffolds of Poly (3-Hydroxybutyric Acid) (PHB) and Poly(3-Hydroxybutyric-co-3-Hydroxyvaleric Acid) (PHBV) Through Non-Invasive Color Staining Method

Polyhydroxyalkanoates (PHAs) has been investigated for more than eighty years. Ever since then, the scientists are kept on synthesizing and developing new polymers and application to suit human interests nowadays. The resourcefulness of PHAs has made them a good candidates for the study of their pot...

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Main Authors:	Saiful Irwan Zubairi, Mantalaris, Athanasios, Bismarck, Alexander
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/9271/ http://journalarticle.ukm.my/9271/ http://journalarticle.ukm.my/9271/1/18_Saiful_Irwan_Zubairi.pdf

id	ukm-9271
recordtype	eprints
spelling	ukm-92712016-12-14T06:49:25Z http://journalarticle.ukm.my/9271/ Pore Interconnectivity Analysis of Porous Three Dimensional Scaffolds of Poly (3-Hydroxybutyric Acid) (PHB) and Poly(3-Hydroxybutyric-co-3-Hydroxyvaleric Acid) (PHBV) Through Non-Invasive Color Staining Method Saiful Irwan Zubairi, Mantalaris, Athanasios Bismarck, Alexander Polyhydroxyalkanoates (PHAs) has been investigated for more than eighty years. Ever since then, the scientists are kept on synthesizing and developing new polymers and application to suit human interests nowadays. The resourcefulness of PHAs has made them a good candidates for the study of their potential in a variety of areas from biomedical/medical fields to food, packaging, textile and household material. In medical field (specifically in tissue engineering application), producing a biocompatible 3-D scaffold with adaptable physical properties are essential. However, to the best of our knowledge, scaffolds from PHB and PHBV with thickness greater than 1 mm have not been produced yet. In this work, PHB and PHBV porous 3-D scaffolds with an improved thickness greater than 4 mm was fabricated using conventional method of solvent-casting particulate-leaching (SCPL). A preliminary assessment on the improved thickness 3-D scaffolds was carried out to examine its pore interconnectivity by using non-invasive color staining method. The vertical cross sections image of the stained scaffolds was analyzed by image analyzer software. This technique was considered simple, fast and cost effective method prior to the usage of super accurate analytical instruments (micro-computed tomography). The results showed that over 80% of the pores have been interconnected with the adjacent pores. Moreover, there was a good correlation between the predicted pore interconnectivity and porosity. These results indicated how well a simple technique can do by giving an overview of the internal morphology of a porous 3-D structure material. Universiti Kebangsaan Malaysia 2015-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9271/1/18_Saiful_Irwan_Zubairi.pdf Saiful Irwan Zubairi, and Mantalaris, Athanasios and Bismarck, Alexander (2015) Pore Interconnectivity Analysis of Porous Three Dimensional Scaffolds of Poly (3-Hydroxybutyric Acid) (PHB) and Poly(3-Hydroxybutyric-co-3-Hydroxyvaleric Acid) (PHBV) Through Non-Invasive Color Staining Method. Sains Malaysiana, 44 (9). pp. 1351-1356. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid44bil9_2015/KandunganJilid44Bil9_2015.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Polyhydroxyalkanoates (PHAs) has been investigated for more than eighty years. Ever since then, the scientists are kept on synthesizing and developing new polymers and application to suit human interests nowadays. The resourcefulness of PHAs has made them a good candidates for the study of their potential in a variety of areas from biomedical/medical fields to food, packaging, textile and household material. In medical field (specifically in tissue engineering application), producing a biocompatible 3-D scaffold with adaptable physical properties are essential. However, to the best of our knowledge, scaffolds from PHB and PHBV with thickness greater than 1 mm have not been produced yet. In this work, PHB and PHBV porous 3-D scaffolds with an improved thickness greater than 4 mm was fabricated using conventional method of solvent-casting particulate-leaching (SCPL). A preliminary assessment on the improved thickness 3-D scaffolds was carried out to examine its pore interconnectivity by using non-invasive color staining method. The vertical cross sections image of the stained scaffolds was analyzed by image analyzer software. This technique was considered simple, fast and cost effective method prior to the usage of super accurate analytical instruments (micro-computed tomography). The results showed that over 80% of the pores have been interconnected with the adjacent pores. Moreover, there was a good correlation between the predicted pore interconnectivity and porosity. These results indicated how well a simple technique can do by giving an overview of the internal morphology of a porous 3-D structure material.
format	Article
author	Saiful Irwan Zubairi, Mantalaris, Athanasios Bismarck, Alexander
spellingShingle	Saiful Irwan Zubairi, Mantalaris, Athanasios Bismarck, Alexander Pore Interconnectivity Analysis of Porous Three Dimensional Scaffolds of Poly (3-Hydroxybutyric Acid) (PHB) and Poly(3-Hydroxybutyric-co-3-Hydroxyvaleric Acid) (PHBV) Through Non-Invasive Color Staining Method
author_facet	Saiful Irwan Zubairi, Mantalaris, Athanasios Bismarck, Alexander
author_sort	Saiful Irwan Zubairi,
title	Pore Interconnectivity Analysis of Porous Three Dimensional Scaffolds of Poly (3-Hydroxybutyric Acid) (PHB) and Poly(3-Hydroxybutyric-co-3-Hydroxyvaleric Acid) (PHBV) Through Non-Invasive Color Staining Method
title_short	Pore Interconnectivity Analysis of Porous Three Dimensional Scaffolds of Poly (3-Hydroxybutyric Acid) (PHB) and Poly(3-Hydroxybutyric-co-3-Hydroxyvaleric Acid) (PHBV) Through Non-Invasive Color Staining Method
title_full	Pore Interconnectivity Analysis of Porous Three Dimensional Scaffolds of Poly (3-Hydroxybutyric Acid) (PHB) and Poly(3-Hydroxybutyric-co-3-Hydroxyvaleric Acid) (PHBV) Through Non-Invasive Color Staining Method
title_fullStr	Pore Interconnectivity Analysis of Porous Three Dimensional Scaffolds of Poly (3-Hydroxybutyric Acid) (PHB) and Poly(3-Hydroxybutyric-co-3-Hydroxyvaleric Acid) (PHBV) Through Non-Invasive Color Staining Method
title_full_unstemmed	Pore Interconnectivity Analysis of Porous Three Dimensional Scaffolds of Poly (3-Hydroxybutyric Acid) (PHB) and Poly(3-Hydroxybutyric-co-3-Hydroxyvaleric Acid) (PHBV) Through Non-Invasive Color Staining Method
title_sort	pore interconnectivity analysis of porous three dimensional scaffolds of poly (3-hydroxybutyric acid) (phb) and poly(3-hydroxybutyric-co-3-hydroxyvaleric acid) (phbv) through non-invasive color staining method
publisher	Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/9271/ http://journalarticle.ukm.my/9271/ http://journalarticle.ukm.my/9271/1/18_Saiful_Irwan_Zubairi.pdf
first_indexed	2023-09-18T19:54:25Z
last_indexed	2023-09-18T19:54:25Z
_version_	1777406433175797760

Pore Interconnectivity Analysis of Porous Three Dimensional Scaffolds of Poly (3-Hydroxybutyric Acid) (PHB) and Poly(3-Hydroxybutyric-co-3-Hydroxyvaleric Acid) (PHBV) Through Non-Invasive Color Staining Method

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