The Estimation of Foetomaternal Haemorrhage by Flowcytometry

Fetomaternal haemorrhage (FMH) may occur following a sensitizing event, during pregnancy or at delivery. In cases of rhesus (Rh) incompatibility between mother and the fetus, it can thus subject to the haemolytic disease of the newborn. The Kleihauer test for quantification of FMH lacks standardizat...

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Main Authors:	Nurasyikin Y, Suria AA, Soon, Peng Ng, Leong, CF
Format:	Article
Language:	English
Published:	Penerbit UKM 2015
Online Access:	http://journalarticle.ukm.my/9278/ http://journalarticle.ukm.my/9278/ http://journalarticle.ukm.my/9278/1/5.%2520Nurasyikin%2520Y%2520et%2520al..pdf

id	ukm-9278
recordtype	eprints
spelling	ukm-92782016-12-14T06:49:26Z http://journalarticle.ukm.my/9278/ The Estimation of Foetomaternal Haemorrhage by Flowcytometry Nurasyikin Y, Suria AA, Soon, Peng Ng Leong, CF Fetomaternal haemorrhage (FMH) may occur following a sensitizing event, during pregnancy or at delivery. In cases of rhesus (Rh) incompatibility between mother and the fetus, it can thus subject to the haemolytic disease of the newborn. The Kleihauer test for quantification of FMH lacks standardization and results are less accurate. Furthermore, it cannot differentiate the fetal cell from the adult HbF. Flowcytometry analysis using monoclonal antibodies, is a new technique for the quantification of FMH and it allows larger number of cells to be analysed. It is also able to differentiate the fetal cell from maternal HbF, and thus is more sensitive and accurate. The objective of our study was to determine the FMH using the flowcytometric analysis of anti-HbF antibody and to correlate the FMH using flow cytometry and the standard Kleihauer test. Ninety eight peripheral blood samples from pregnant women at more than 20 weeks of pregnancy and post delivery were analyzed by both methods. The percentage of the fetal cells were recorded and the FMH were calculated. We found a fair correlation between the two methods with the correlation coefficient r = 0.633 (p<0.05). The concordance rate was 66.3%. Flow cytometric method, however, gave higher values in 70.4% of cases as compared to the Kleihauer test. These could be due to the autofluoresce of contaminated white blood cell or using anti HbF which is less specific than anti-D. Other possible factors could be due to the fluorochrome used. Therefore, in order to increase the accuracy, we recommend the use of dual labeling of red cells with glycophorin A which is a marker for red cells and compare the use of monoclonal anti HbF and anti-D for analysis. Penerbit UKM 2015-12-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9278/1/5.%2520Nurasyikin%2520Y%2520et%2520al..pdf Nurasyikin Y, and Suria AA, and Soon, Peng Ng and Leong, CF (2015) The Estimation of Foetomaternal Haemorrhage by Flowcytometry. Medicine & Health, 10 (2). pp. 112-122. ISSN 1823-2140 http://www.medicineandhealthukm.com
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Fetomaternal haemorrhage (FMH) may occur following a sensitizing event, during pregnancy or at delivery. In cases of rhesus (Rh) incompatibility between mother and the fetus, it can thus subject to the haemolytic disease of the newborn. The Kleihauer test for quantification of FMH lacks standardization and results are less accurate. Furthermore, it cannot differentiate the fetal cell from the adult HbF. Flowcytometry analysis using monoclonal antibodies, is a new technique for the quantification of FMH and it allows larger number of cells to be analysed. It is also able to differentiate the fetal cell from maternal HbF, and thus is more sensitive and accurate. The objective of our study was to determine the FMH using the flowcytometric analysis of anti-HbF antibody and to correlate the FMH using flow cytometry and the standard Kleihauer test. Ninety eight peripheral blood samples from pregnant women at more than 20 weeks of pregnancy and post delivery were analyzed by both methods. The percentage of the fetal cells were recorded and the FMH were calculated. We found a fair correlation between the two methods with the correlation coefficient r = 0.633 (p<0.05). The concordance rate was 66.3%. Flow cytometric method, however, gave higher values in 70.4% of cases as compared to the Kleihauer test. These could be due to the autofluoresce of contaminated white blood cell or using anti HbF which is less specific than anti-D. Other possible factors could be due to the fluorochrome used. Therefore, in order to increase the accuracy, we recommend the use of dual labeling of red cells with glycophorin A which is a marker for red cells and compare the use of monoclonal anti HbF and anti-D for analysis.
format	Article
author	Nurasyikin Y, Suria AA, Soon, Peng Ng Leong, CF
spellingShingle	Nurasyikin Y, Suria AA, Soon, Peng Ng Leong, CF The Estimation of Foetomaternal Haemorrhage by Flowcytometry
author_facet	Nurasyikin Y, Suria AA, Soon, Peng Ng Leong, CF
author_sort	Nurasyikin Y,
title	The Estimation of Foetomaternal Haemorrhage by Flowcytometry
title_short	The Estimation of Foetomaternal Haemorrhage by Flowcytometry
title_full	The Estimation of Foetomaternal Haemorrhage by Flowcytometry
title_fullStr	The Estimation of Foetomaternal Haemorrhage by Flowcytometry
title_full_unstemmed	The Estimation of Foetomaternal Haemorrhage by Flowcytometry
title_sort	estimation of foetomaternal haemorrhage by flowcytometry
publisher	Penerbit UKM
publishDate	2015
url	http://journalarticle.ukm.my/9278/ http://journalarticle.ukm.my/9278/ http://journalarticle.ukm.my/9278/1/5.%2520Nurasyikin%2520Y%2520et%2520al..pdf
first_indexed	2023-09-18T19:54:26Z
last_indexed	2023-09-18T19:54:26Z
_version_	1777406434202353664

The Estimation of Foetomaternal Haemorrhage by Flowcytometry

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