A study of the PfNT3 in Plasmodium falciparum

Previous genetic studies demonstrated that survival and proliferation of Plasmodium falciparum parasites is dependent on salvage of essential purines from the host. Plasmodium falciparum, the causative agent of the most lethal form of human malaria lacks the enzymes required for de novo synthesis of...

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Main Authors:	Sahu Pratima Kumari, Panda Maheswar, Patra Satyajit, Das Sidhartha, Satyamoorthy K, Mohanty Dipika
Format:	Article
Language:	English
Published:	Penerbit UKM 2015
Online Access:	http://journalarticle.ukm.my/9279/ http://journalarticle.ukm.my/9279/ http://journalarticle.ukm.my/9279/1/6.%2520Sahu%2520Pratima%2520Kumari%2520et%2520al..pdf

id	ukm-9279
recordtype	eprints
spelling	ukm-92792016-12-14T06:49:26Z http://journalarticle.ukm.my/9279/ A study of the PfNT3 in Plasmodium falciparum Sahu Pratima Kumari, Panda Maheswar, Patra Satyajit, Das Sidhartha, Satyamoorthy K, Mohanty Dipika, Previous genetic studies demonstrated that survival and proliferation of Plasmodium falciparum parasites is dependent on salvage of essential purines from the host. Plasmodium falciparum, the causative agent of the most lethal form of human malaria lacks the enzymes required for de novo synthesis of purines. Analysis of the hypothetical nucleoside/nucleobase transporter protein, the gene product of PfNT3 (PF14_0662) gene in P. falciparum parasites was carried out by localisation, in view of a novel chemotherapeutic target. Immunoblotting, immunofluorescent and immunoelectron microscopic localization of PfNT3 was demonstrated using polyclonal antiserum in in vitro cultured Plasmodium falciparum parasites, propagated in human red blood cells. PfNT3 protein, the translated product of PfNT3 gene was detected in intraerythrocytic ring, trophozoite, and schizont stages. PfNT3 was localized primarily to the PPM (Parasite Plasma Membrane). The endogenous PfNT3 putative nucleoside transporter with the predominant location to the parasite plasma membrane may serve not only as routes for targeting of purine analogs/cytotoxic agents into the intracellular parasite but may also serve as drug targets. Being genome encoded the vital transporter protein can be prevented from expression by silencing of the gene, validating it to be a novel drug target. Penerbit UKM 2015-12-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9279/1/6.%2520Sahu%2520Pratima%2520Kumari%2520et%2520al..pdf Sahu Pratima Kumari, and Panda Maheswar, and Patra Satyajit, and Das Sidhartha, and Satyamoorthy K, and Mohanty Dipika, (2015) A study of the PfNT3 in Plasmodium falciparum. Medicine & Health, 10 (2). pp. 123-136. ISSN 1823-2140 http://www.medicineandhealthukm.com
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Previous genetic studies demonstrated that survival and proliferation of Plasmodium falciparum parasites is dependent on salvage of essential purines from the host. Plasmodium falciparum, the causative agent of the most lethal form of human malaria lacks the enzymes required for de novo synthesis of purines. Analysis of the hypothetical nucleoside/nucleobase transporter protein, the gene product of PfNT3 (PF14_0662) gene in P. falciparum parasites was carried out by localisation, in view of a novel chemotherapeutic target. Immunoblotting, immunofluorescent and immunoelectron microscopic localization of PfNT3 was demonstrated using polyclonal antiserum in in vitro cultured Plasmodium falciparum parasites, propagated in human red blood cells. PfNT3 protein, the translated product of PfNT3 gene was detected in intraerythrocytic ring, trophozoite, and schizont stages. PfNT3 was localized primarily to the PPM (Parasite Plasma Membrane). The endogenous PfNT3 putative nucleoside transporter with the predominant location to the parasite plasma membrane may serve not only as routes for targeting of purine analogs/cytotoxic agents into the intracellular parasite but may also serve as drug targets. Being genome encoded the vital transporter protein can be prevented from expression by silencing of the gene, validating it to be a novel drug target.
format	Article
author	Sahu Pratima Kumari, Panda Maheswar, Patra Satyajit, Das Sidhartha, Satyamoorthy K, Mohanty Dipika,
spellingShingle	Sahu Pratima Kumari, Panda Maheswar, Patra Satyajit, Das Sidhartha, Satyamoorthy K, Mohanty Dipika, A study of the PfNT3 in Plasmodium falciparum
author_facet	Sahu Pratima Kumari, Panda Maheswar, Patra Satyajit, Das Sidhartha, Satyamoorthy K, Mohanty Dipika,
author_sort	Sahu Pratima Kumari,
title	A study of the PfNT3 in Plasmodium falciparum
title_short	A study of the PfNT3 in Plasmodium falciparum
title_full	A study of the PfNT3 in Plasmodium falciparum
title_fullStr	A study of the PfNT3 in Plasmodium falciparum
title_full_unstemmed	A study of the PfNT3 in Plasmodium falciparum
title_sort	study of the pfnt3 in plasmodium falciparum
publisher	Penerbit UKM
publishDate	2015
url	http://journalarticle.ukm.my/9279/ http://journalarticle.ukm.my/9279/ http://journalarticle.ukm.my/9279/1/6.%2520Sahu%2520Pratima%2520Kumari%2520et%2520al..pdf
first_indexed	2023-09-18T19:54:26Z
last_indexed	2023-09-18T19:54:26Z
_version_	1777406434344960000

A study of the PfNT3 in Plasmodium falciparum

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