The establishment of a primary colon carcinoma cell line

A primary human colon carcinoma cell line (IMR22) was established in the Institute for Medical Research from a 71 years old Chinese m,an who had a right Hemicolectomy done for a fungating mass in the carcum. The tumour mass was then confirmed histopathologically to be a well differentiated Adenocarc...

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Main Authors:	Zubaidah Zakaria, Gregory A.R.A., Tong. S, Boon. C.F, Asril Yusof, Norhanom Abdul Wahab, Latifah Saiful Yazan, Muhammad Saifulamman Mohamed Said
Format:	Article
Language:	English
Published:	penerbit ukm 2003
Online Access:	http://journalarticle.ukm.my/945/ http://journalarticle.ukm.my/945/ http://journalarticle.ukm.my/945/1/jurnal.pdf

id	ukm-945
recordtype	eprints
spelling	ukm-9452016-12-14T06:28:22Z http://journalarticle.ukm.my/945/ The establishment of a primary colon carcinoma cell line Zubaidah Zakaria, Gregory A.R.A., Tong. S, Boon. C.F, Asril Yusof, Norhanom Abdul Wahab, Latifah Saiful Yazan, Muhammad Saifulamman Mohamed Said, A primary human colon carcinoma cell line (IMR22) was established in the Institute for Medical Research from a 71 years old Chinese m,an who had a right Hemicolectomy done for a fungating mass in the carcum. The tumour mass was then confirmed histopathologically to be a well differentiated Adenocarcinoma Dukes Stage II, Apiece of the tumour tissue resected fresh from the operating theatre was grown to extablish the cell line. The initial growth medium was in Amniomax C 100 supplemented with Fungizone, Penicillin and Streplomycin and was later changed to Medium 199 with Earl’s salt supplemented with 20% fetal calf serum, Kanamycin, Penicillin and Streptomycin and Lgtutamine. The IMR22 line established after ten passages with the occurrence of single type of cell in the culture whose morphological characteristics resembled that of the primary tumour. penerbit ukm 2003-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/945/1/jurnal.pdf Zubaidah Zakaria, and Gregory A.R.A., and Tong. S, and Boon. C.F, and Asril Yusof, and Norhanom Abdul Wahab, and Latifah Saiful Yazan, and Muhammad Saifulamman Mohamed Said, (2003) The establishment of a primary colon carcinoma cell line. Jurnal Sains Kesihatan Malaysia, 1 . pp. 35-43. ISSN 1675-8161 http://www.fskb.ukm.my/fskbold/jurnal
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	A primary human colon carcinoma cell line (IMR22) was established in the Institute for Medical Research from a 71 years old Chinese m,an who had a right Hemicolectomy done for a fungating mass in the carcum. The tumour mass was then confirmed histopathologically to be a well differentiated Adenocarcinoma Dukes Stage II, Apiece of the tumour tissue resected fresh from the operating theatre was grown to extablish the cell line. The initial growth medium was in Amniomax C 100 supplemented with Fungizone, Penicillin and Streplomycin and was later changed to Medium 199 with Earl’s salt supplemented with 20% fetal calf serum, Kanamycin, Penicillin and Streptomycin and Lgtutamine. The IMR22 line established after ten passages with the occurrence of single type of cell in the culture whose morphological characteristics resembled that of the primary tumour.
format	Article
author	Zubaidah Zakaria, Gregory A.R.A., Tong. S, Boon. C.F, Asril Yusof, Norhanom Abdul Wahab, Latifah Saiful Yazan, Muhammad Saifulamman Mohamed Said,
spellingShingle	Zubaidah Zakaria, Gregory A.R.A., Tong. S, Boon. C.F, Asril Yusof, Norhanom Abdul Wahab, Latifah Saiful Yazan, Muhammad Saifulamman Mohamed Said, The establishment of a primary colon carcinoma cell line
author_facet	Zubaidah Zakaria, Gregory A.R.A., Tong. S, Boon. C.F, Asril Yusof, Norhanom Abdul Wahab, Latifah Saiful Yazan, Muhammad Saifulamman Mohamed Said,
author_sort	Zubaidah Zakaria,
title	The establishment of a primary colon carcinoma cell line
title_short	The establishment of a primary colon carcinoma cell line
title_full	The establishment of a primary colon carcinoma cell line
title_fullStr	The establishment of a primary colon carcinoma cell line
title_full_unstemmed	The establishment of a primary colon carcinoma cell line
title_sort	establishment of a primary colon carcinoma cell line
publisher	penerbit ukm
publishDate	2003
url	http://journalarticle.ukm.my/945/ http://journalarticle.ukm.my/945/ http://journalarticle.ukm.my/945/1/jurnal.pdf
first_indexed	2023-09-18T19:31:58Z
last_indexed	2023-09-18T19:31:58Z
_version_	1777405021225222144

The establishment of a primary colon carcinoma cell line

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