Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines

In this vitro study, the secretion of interleukin-8 (IL-8) and RANTES proteins by the MKN45 gastric epithetial cell line was examined using the ELISA technique after the cells had been stimulated with pro-inflammatory cytokines, I,e,II, I "α" ,TNF-α and IFN-γ, The chemokines demonstrated a...

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Main Authors:	Norina Abdullah, Jordon N.J, Kolios G., Colloins A.J
Format:	Article
Language:	English
Published:	penerbit ukm 2003
Online Access:	http://journalarticle.ukm.my/946/ http://journalarticle.ukm.my/946/ http://journalarticle.ukm.my/946/1/jurnal2.pdf

id	ukm-946
recordtype	eprints
spelling	ukm-9462016-12-14T06:28:23Z http://journalarticle.ukm.my/946/ Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines Norina Abdullah, Jordon N.J, Kolios G., Colloins A.J, In this vitro study, the secretion of interleukin-8 (IL-8) and RANTES proteins by the MKN45 gastric epithetial cell line was examined using the ELISA technique after the cells had been stimulated with pro-inflammatory cytokines, I,e,II, I "α" ,TNF-α and IFN-γ, The chemokines demonstrated a timedependent increase in concentration after stimulation with the various cytokines, alone or in combination, IL-I α was the weakest stimuli while a combination of IL-I α, TNF-α and IFN-γ provided the strongest stimulation. The immunomodulatory effects of IL-4 and IL-13 on the chemokine protein secretion were also studied. Stimulation of the cells with IL-4 and IL-13 for an hour at relatively high concentrations (10 and 30 ng/ml) prior to stimulation with the combination of IL-I α, TNF-α and IFN –γ inhibited the secretion of RANTES protein but did not inhibit the secretion of IL-8 mRNA of protwin. This inplied that the chemokines might have different inbitiory pathways. IL-4 and IL-13 played an important role in decreasing certain inflammatory chemokines and this might have an important effect in decreasing gastric inflammation. penerbit ukm 2003-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/946/1/jurnal2.pdf Norina Abdullah, and Jordon N.J, and Kolios G., and Colloins A.J, (2003) Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines. Jurnal Sains Kesihatan Malaysia, 1 . pp. 9-21. ISSN 1675-8161 http://www.fskb.ukm.my/fskbold/jurnal
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	In this vitro study, the secretion of interleukin-8 (IL-8) and RANTES proteins by the MKN45 gastric epithetial cell line was examined using the ELISA technique after the cells had been stimulated with pro-inflammatory cytokines, I,e,II, I "α" ,TNF-α and IFN-γ, The chemokines demonstrated a timedependent increase in concentration after stimulation with the various cytokines, alone or in combination, IL-I α was the weakest stimuli while a combination of IL-I α, TNF-α and IFN-γ provided the strongest stimulation. The immunomodulatory effects of IL-4 and IL-13 on the chemokine protein secretion were also studied. Stimulation of the cells with IL-4 and IL-13 for an hour at relatively high concentrations (10 and 30 ng/ml) prior to stimulation with the combination of IL-I α, TNF-α and IFN –γ inhibited the secretion of RANTES protein but did not inhibit the secretion of IL-8 mRNA of protwin. This inplied that the chemokines might have different inbitiory pathways. IL-4 and IL-13 played an important role in decreasing certain inflammatory chemokines and this might have an important effect in decreasing gastric inflammation.
format	Article
author	Norina Abdullah, Jordon N.J, Kolios G., Colloins A.J,
spellingShingle	Norina Abdullah, Jordon N.J, Kolios G., Colloins A.J, Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines
author_facet	Norina Abdullah, Jordon N.J, Kolios G., Colloins A.J,
author_sort	Norina Abdullah,
title	Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines
title_short	Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines
title_full	Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines
title_fullStr	Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines
title_full_unstemmed	Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines
title_sort	secretion of rantes and interleukin-8 by the human gastric epithelial cell line (mkn45) through modulation with cytokines
publisher	penerbit ukm
publishDate	2003
url	http://journalarticle.ukm.my/946/ http://journalarticle.ukm.my/946/ http://journalarticle.ukm.my/946/1/jurnal2.pdf
first_indexed	2023-09-18T19:31:58Z
last_indexed	2023-09-18T19:31:58Z
_version_	1777405021367828480

Secretion of RANTES and interleukin-8 by the human gastric epithelial cell line (MKN45) through modulation with cytokines

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