Bioactive constituent characterization and antioxidant activity of ganoderma lucidum extract fractionated by supercritical carbon dioxide

Bioactive constituent characterization and antioxidant activity of ganoderma lucidum extract fractionated by supercritical carbon dioxide

Ganoderma lucidum has been recognized as a precious fungus in both Chinese and Japanese traditional medicine for centuries. It contains many bioactive ingredients such as triterpenoids and polysaccharides. The present study used supercritical carbon dioxide (SC-CO2) fractionation to fractionate Gano...

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Main Authors: Mei, Su Lin, Zer, Ran Yu, Be, Jen Wang, Cheng, Chi Wang, Yih, Ming Weng, Malcolm Koo
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2015
Online Access:http://journalarticle.ukm.my/9489/
http://journalarticle.ukm.my/9489/
http://journalarticle.ukm.my/9489/1/05__Mei-Su_Lin.pdf
id ukm-9489
recordtype eprints
spelling ukm-94892016-12-14T06:50:05Z http://journalarticle.ukm.my/9489/ Bioactive constituent characterization and antioxidant activity of ganoderma lucidum extract fractionated by supercritical carbon dioxide Mei, Su Lin Zer, Ran Yu Be, Jen Wang Cheng, Chi Wang Yih, Ming Weng Malcolm Koo, Ganoderma lucidum has been recognized as a precious fungus in both Chinese and Japanese traditional medicine for centuries. It contains many bioactive ingredients such as triterpenoids and polysaccharides. The present study used supercritical carbon dioxide (SC-CO2) fractionation to fractionate Ganoderma lucidum extract into four fractions (R, F1, F2, & F3) and evaluate the correlation between the content of functional components and their antioxidant ability. Relatively high concentrations of the three types of bioactive constituents were simultaneously partitioned into different fractionation collecting vessels. The free radical scavenging ability was greatest in F1. The IC50 of DPPH scavenging ability was 0.90 mg/mL and that of ABTS radicals scavenging activity was 0.45 mg/mL. The correlation analysis of antioxidant ability with total triterpenoids and total polyphenols showed a positive relationship. In conclusion, this study showed that fractionation of Ganoderma lucidum extract using SC-CO2 fractionation technology was able to effectively partition its bioactive components including triterpenoids, polysaccharides and phenolic compounds and also to increase the antioxidant activities of the fractions. Universiti Kebangsaan Malaysia 2015-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9489/1/05__Mei-Su_Lin.pdf Mei, Su Lin and Zer, Ran Yu and Be, Jen Wang and Cheng, Chi Wang and Yih, Ming Weng and Malcolm Koo, (2015) Bioactive constituent characterization and antioxidant activity of ganoderma lucidum extract fractionated by supercritical carbon dioxide. Sains Malaysiana, 44 (12). pp. 1685-1691. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol44num12_2015/contentsVol44num12_2015.html
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language English
description Ganoderma lucidum has been recognized as a precious fungus in both Chinese and Japanese traditional medicine for centuries. It contains many bioactive ingredients such as triterpenoids and polysaccharides. The present study used supercritical carbon dioxide (SC-CO2) fractionation to fractionate Ganoderma lucidum extract into four fractions (R, F1, F2, & F3) and evaluate the correlation between the content of functional components and their antioxidant ability. Relatively high concentrations of the three types of bioactive constituents were simultaneously partitioned into different fractionation collecting vessels. The free radical scavenging ability was greatest in F1. The IC50 of DPPH scavenging ability was 0.90 mg/mL and that of ABTS radicals scavenging activity was 0.45 mg/mL. The correlation analysis of antioxidant ability with total triterpenoids and total polyphenols showed a positive relationship. In conclusion, this study showed that fractionation of Ganoderma lucidum extract using SC-CO2 fractionation technology was able to effectively partition its bioactive components including triterpenoids, polysaccharides and phenolic compounds and also to increase the antioxidant activities of the fractions.
format Article
author Mei, Su Lin
Zer, Ran Yu
Be, Jen Wang
Cheng, Chi Wang
Yih, Ming Weng
Malcolm Koo,
spellingShingle Mei, Su Lin
Zer, Ran Yu
Be, Jen Wang
Cheng, Chi Wang
Yih, Ming Weng
Malcolm Koo,
Bioactive constituent characterization and antioxidant activity of ganoderma lucidum extract fractionated by supercritical carbon dioxide
author_facet Mei, Su Lin
Zer, Ran Yu
Be, Jen Wang
Cheng, Chi Wang
Yih, Ming Weng
Malcolm Koo,
author_sort Mei, Su Lin
title Bioactive constituent characterization and antioxidant activity of ganoderma lucidum extract fractionated by supercritical carbon dioxide
title_short Bioactive constituent characterization and antioxidant activity of ganoderma lucidum extract fractionated by supercritical carbon dioxide
title_full Bioactive constituent characterization and antioxidant activity of ganoderma lucidum extract fractionated by supercritical carbon dioxide
title_fullStr Bioactive constituent characterization and antioxidant activity of ganoderma lucidum extract fractionated by supercritical carbon dioxide
title_full_unstemmed Bioactive constituent characterization and antioxidant activity of ganoderma lucidum extract fractionated by supercritical carbon dioxide
title_sort bioactive constituent characterization and antioxidant activity of ganoderma lucidum extract fractionated by supercritical carbon dioxide
publisher Universiti Kebangsaan Malaysia
publishDate 2015
url http://journalarticle.ukm.my/9489/
http://journalarticle.ukm.my/9489/
http://journalarticle.ukm.my/9489/1/05__Mei-Su_Lin.pdf
first_indexed 2023-09-18T19:55:02Z
last_indexed 2023-09-18T19:55:02Z
_version_ 1777406472308654080

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