DNA ploidy analysis on lymphoma using archival paraffin tissue section

In this retrospective study of lymphomas, DNA content and proliferative index of 37 histopathologically confirmed cases of lymphoma consisting of 29 male and 8 female were measured using a flowcytometer. This was carried out using paraffin embedded tissues of cases diagnosed in the Department of Pat...

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Main Authors:	Norizan Mohd Annuar, Gregory A.R.A
Format:	Article
Language:	English
Published:	penerbit ukm 2004
Online Access:	http://journalarticle.ukm.my/953/ http://journalarticle.ukm.my/953/ http://journalarticle.ukm.my/953/1/jurnal9.pdf

id	ukm-953
recordtype	eprints
spelling	ukm-9532016-12-14T06:28:24Z http://journalarticle.ukm.my/953/ DNA ploidy analysis on lymphoma using archival paraffin tissue section Norizan Mohd Annuar, Gregory A.R.A, In this retrospective study of lymphomas, DNA content and proliferative index of 37 histopathologically confirmed cases of lymphoma consisting of 29 male and 8 female were measured using a flowcytometer. This was carried out using paraffin embedded tissues of cases diagnosed in the Department of Pathology, Hospital Kuala Lumpur, over a period of 1990 to 1995. Among them, 24 were diagnosed to be non-Hodgkin’s lymphomas (NHL) while 13 were diagnosed as Hodgkin’s disease (HD). Lymph nodes showing reactive changes and tonsils removed for chronic inflammation were used as control. Of all the cases studied for NHL (B cell = 70.8%, T cell = 8.3% and unclassified cases = 20.9%), 12.5% were near diploid (DNA index, DI = 1.0 - 1.10), 66.7% were hyperdiploid (DI = 1.10 - 1.85) and 20.8% were hypodiploid (DI < 1.0). Majority of the hyperdiploid cases were B cell type of NHL. In HD (mixed cellularity = 1, nodular sclerosing = 5, lymphocyte predominant = 1 and unclassified = 6) 30.8% were near diploid, 46.1% were hyperdiploid and 23.1% were hypodiploid. The significant finding of this study was that the majority of the NHL and HD showed a high proliferative index. penerbit ukm 2004 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/953/1/jurnal9.pdf Norizan Mohd Annuar, and Gregory A.R.A, (2004) DNA ploidy analysis on lymphoma using archival paraffin tissue section. Jurnal Sains Kesihatan Malaysia, 2 (1). pp. 57-64. ISSN 1675-8161 http://www.fskb.ukm.my/fskbold/jurnal
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	In this retrospective study of lymphomas, DNA content and proliferative index of 37 histopathologically confirmed cases of lymphoma consisting of 29 male and 8 female were measured using a flowcytometer. This was carried out using paraffin embedded tissues of cases diagnosed in the Department of Pathology, Hospital Kuala Lumpur, over a period of 1990 to 1995. Among them, 24 were diagnosed to be non-Hodgkin’s lymphomas (NHL) while 13 were diagnosed as Hodgkin’s disease (HD). Lymph nodes showing reactive changes and tonsils removed for chronic inflammation were used as control. Of all the cases studied for NHL (B cell = 70.8%, T cell = 8.3% and unclassified cases = 20.9%), 12.5% were near diploid (DNA index, DI = 1.0 - 1.10), 66.7% were hyperdiploid (DI = 1.10 - 1.85) and 20.8% were hypodiploid (DI < 1.0). Majority of the hyperdiploid cases were B cell type of NHL. In HD (mixed cellularity = 1, nodular sclerosing = 5, lymphocyte predominant = 1 and unclassified = 6) 30.8% were near diploid, 46.1% were hyperdiploid and 23.1% were hypodiploid. The significant finding of this study was that the majority of the NHL and HD showed a high proliferative index.
format	Article
author	Norizan Mohd Annuar, Gregory A.R.A,
spellingShingle	Norizan Mohd Annuar, Gregory A.R.A, DNA ploidy analysis on lymphoma using archival paraffin tissue section
author_facet	Norizan Mohd Annuar, Gregory A.R.A,
author_sort	Norizan Mohd Annuar,
title	DNA ploidy analysis on lymphoma using archival paraffin tissue section
title_short	DNA ploidy analysis on lymphoma using archival paraffin tissue section
title_full	DNA ploidy analysis on lymphoma using archival paraffin tissue section
title_fullStr	DNA ploidy analysis on lymphoma using archival paraffin tissue section
title_full_unstemmed	DNA ploidy analysis on lymphoma using archival paraffin tissue section
title_sort	dna ploidy analysis on lymphoma using archival paraffin tissue section
publisher	penerbit ukm
publishDate	2004
url	http://journalarticle.ukm.my/953/ http://journalarticle.ukm.my/953/ http://journalarticle.ukm.my/953/1/jurnal9.pdf
first_indexed	2023-09-18T19:31:59Z
last_indexed	2023-09-18T19:31:59Z
_version_	1777405022381801472

DNA ploidy analysis on lymphoma using archival paraffin tissue section

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