Matrine enhances the inhibitory effect of 5-FU on SW480 cells in vitro and in vivo

We investigated the antitumor effects of the combination of matrine-a purified alkaloid extracted from Sophora flavescence-and 5-fluorouracil (5-FU) on SW480 cells. This combination inhibited the growth of SW480 cells in a synergistic or additive manner by disrupting their progression through the ce...

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Main Authors:	Xu, Hui Zhang, Lei, Liang, Xiao, Yan Wang, Li, Zhang, Yan, Xin Zheng, Hong, Zhu Deng, Xin, Rong Wu
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2016
Online Access:	http://journalarticle.ukm.my/9575/ http://journalarticle.ukm.my/9575/ http://journalarticle.ukm.my/9575/1/13_Lei_Liang.pdf

id	ukm-9575
recordtype	eprints
spelling	ukm-95752016-12-14T06:50:21Z http://journalarticle.ukm.my/9575/ Matrine enhances the inhibitory effect of 5-FU on SW480 cells in vitro and in vivo Xu, Hui Zhang Lei, Liang Xiao, Yan Wang Li, Zhang Yan, Xin Zheng Hong, Zhu Deng Xin, Rong Wu We investigated the antitumor effects of the combination of matrine-a purified alkaloid extracted from Sophora flavescence-and 5-fluorouracil (5-FU) on SW480 cells. This combination inhibited the growth of SW480 cells in a synergistic or additive manner by disrupting their progression through the cell cycle. Exposure of SW480 cells to matrine and 5-FU was followed by an increased rate of expression for caspase-3, caspase-9 and poly-ADP ribose polymerase (PARP) and inhibited the subcutaneous transplantation of SW480 tumors into Balb/c nude mice. Histopathological analysis showed that this effect was most pronounced in the spleens of treated animals. Typical cytotoxic effects observed in 5-FU-treated mice included fibrosis and lymphopenia, whereas in mice treated with 5-FU combined with matrine, the spleen ultrastructure remained intact. These findings indicate that matrine may enhance the therapeutic effectiveness of 5-FU in SW480 tumors by enhancing apoptosis and overcome the threat to immunocompetence associated with 5-FU. Universiti Kebangsaan Malaysia 2016-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9575/1/13_Lei_Liang.pdf Xu, Hui Zhang and Lei, Liang and Xiao, Yan Wang and Li, Zhang and Yan, Xin Zheng and Hong, Zhu Deng and Xin, Rong Wu (2016) Matrine enhances the inhibitory effect of 5-FU on SW480 cells in vitro and in vivo. Sains Malaysiana, 45 (1). pp. 109-113. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid45bil1_2016/KandunganJilid45Bil1_2016.htm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	We investigated the antitumor effects of the combination of matrine-a purified alkaloid extracted from Sophora flavescence-and 5-fluorouracil (5-FU) on SW480 cells. This combination inhibited the growth of SW480 cells in a synergistic or additive manner by disrupting their progression through the cell cycle. Exposure of SW480 cells to matrine and 5-FU was followed by an increased rate of expression for caspase-3, caspase-9 and poly-ADP ribose polymerase (PARP) and inhibited the subcutaneous transplantation of SW480 tumors into Balb/c nude mice. Histopathological analysis showed that this effect was most pronounced in the spleens of treated animals. Typical cytotoxic effects observed in 5-FU-treated mice included fibrosis and lymphopenia, whereas in mice treated with 5-FU combined with matrine, the spleen ultrastructure remained intact. These findings indicate that matrine may enhance the therapeutic effectiveness of 5-FU in SW480 tumors by enhancing apoptosis and overcome the threat to immunocompetence associated with 5-FU.
format	Article
author	Xu, Hui Zhang Lei, Liang Xiao, Yan Wang Li, Zhang Yan, Xin Zheng Hong, Zhu Deng Xin, Rong Wu
spellingShingle	Xu, Hui Zhang Lei, Liang Xiao, Yan Wang Li, Zhang Yan, Xin Zheng Hong, Zhu Deng Xin, Rong Wu Matrine enhances the inhibitory effect of 5-FU on SW480 cells in vitro and in vivo
author_facet	Xu, Hui Zhang Lei, Liang Xiao, Yan Wang Li, Zhang Yan, Xin Zheng Hong, Zhu Deng Xin, Rong Wu
author_sort	Xu, Hui Zhang
title	Matrine enhances the inhibitory effect of 5-FU on SW480 cells in vitro and in vivo
title_short	Matrine enhances the inhibitory effect of 5-FU on SW480 cells in vitro and in vivo
title_full	Matrine enhances the inhibitory effect of 5-FU on SW480 cells in vitro and in vivo
title_fullStr	Matrine enhances the inhibitory effect of 5-FU on SW480 cells in vitro and in vivo
title_full_unstemmed	Matrine enhances the inhibitory effect of 5-FU on SW480 cells in vitro and in vivo
title_sort	matrine enhances the inhibitory effect of 5-fu on sw480 cells in vitro and in vivo
publisher	Universiti Kebangsaan Malaysia
publishDate	2016
url	http://journalarticle.ukm.my/9575/ http://journalarticle.ukm.my/9575/ http://journalarticle.ukm.my/9575/1/13_Lei_Liang.pdf
first_indexed	2023-09-18T19:55:16Z
last_indexed	2023-09-18T19:55:16Z
_version_	1777406487391371264

Matrine enhances the inhibitory effect of 5-FU on SW480 cells in vitro and in vivo

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