Influences of 1,2-dichlorobenzene on bacterial community structure in wetland soil

In order to explore the response of the soil microbial community to 1,2-dichlorobenzene exposure in Wetland soil, a short-term (7 weeks) mesocosm study was conducted at four 1,2-dichlorobenzene concentrations of (100, 400 and 800) μg g -1. Dry soil, sterile and unsterile controls were also compared....

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Main Authors:	Huixing, Liang, Aihui, Chen, Zhaoxia, Li, Muhammad Aqeel Ashraf, Cheng, Ding
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2016
Online Access:	http://journalarticle.ukm.my/9578/ http://journalarticle.ukm.my/9578/ http://journalarticle.ukm.my/9578/1/16_Huixing_Liang.pdf

id	ukm-9578
recordtype	eprints
spelling	ukm-95782016-12-14T06:50:21Z http://journalarticle.ukm.my/9578/ Influences of 1,2-dichlorobenzene on bacterial community structure in wetland soil Huixing, Liang Aihui, Chen Zhaoxia, Li Muhammad Aqeel Ashraf, Cheng, Ding In order to explore the response of the soil microbial community to 1,2-dichlorobenzene exposure in Wetland soil, a short-term (7 weeks) mesocosm study was conducted at four 1,2-dichlorobenzene concentrations of (100, 400 and 800) μg g -1. Dry soil, sterile and unsterile controls were also compared. The results obtained showed a significant effect of 1,2-dichlorobenzene on the development of bacterial populations in soils contaminated with different concentrations of 1,2-dichlorobenzene at the early time after application. In general, however, the number of populations of the same soil sample treated with the same concentration of 1,2-dichlorobenzene differed significantly with the increasing incubation time within the early 5 weeks. The scale of differences in banding patterns-showed that the microbial community structures of 1,2-dichlorobenzene-treated and non-1,2-dichlorobenzene-treated soils were not significantly different after 7 weeks of incubation. DNA in application-responsive bands from the 1,2-dichlorobenzene treatments was recovered and amplified using the universal primers. PCR products were recovered and cloned into pGEM-T Easy (Promega) and two clones were obtained. The two clones were sequenced using the automated Model 3730 DNA sequencing system. The two cloned sequences had very high similarities to an uncultured bacterium reported previously in the database of NCBI. Universiti Kebangsaan Malaysia 2016-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9578/1/16_Huixing_Liang.pdf Huixing, Liang and Aihui, Chen and Zhaoxia, Li and Muhammad Aqeel Ashraf, and Cheng, Ding (2016) Influences of 1,2-dichlorobenzene on bacterial community structure in wetland soil. Sains Malaysiana, 45 (1). pp. 129-134. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol45num1_2016/contentsVol45num1_2016.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	In order to explore the response of the soil microbial community to 1,2-dichlorobenzene exposure in Wetland soil, a short-term (7 weeks) mesocosm study was conducted at four 1,2-dichlorobenzene concentrations of (100, 400 and 800) μg g -1. Dry soil, sterile and unsterile controls were also compared. The results obtained showed a significant effect of 1,2-dichlorobenzene on the development of bacterial populations in soils contaminated with different concentrations of 1,2-dichlorobenzene at the early time after application. In general, however, the number of populations of the same soil sample treated with the same concentration of 1,2-dichlorobenzene differed significantly with the increasing incubation time within the early 5 weeks. The scale of differences in banding patterns-showed that the microbial community structures of 1,2-dichlorobenzene-treated and non-1,2-dichlorobenzene-treated soils were not significantly different after 7 weeks of incubation. DNA in application-responsive bands from the 1,2-dichlorobenzene treatments was recovered and amplified using the universal primers. PCR products were recovered and cloned into pGEM-T Easy (Promega) and two clones were obtained. The two clones were sequenced using the automated Model 3730 DNA sequencing system. The two cloned sequences had very high similarities to an uncultured bacterium reported previously in the database of NCBI.
format	Article
author	Huixing, Liang Aihui, Chen Zhaoxia, Li Muhammad Aqeel Ashraf, Cheng, Ding
spellingShingle	Huixing, Liang Aihui, Chen Zhaoxia, Li Muhammad Aqeel Ashraf, Cheng, Ding Influences of 1,2-dichlorobenzene on bacterial community structure in wetland soil
author_facet	Huixing, Liang Aihui, Chen Zhaoxia, Li Muhammad Aqeel Ashraf, Cheng, Ding
author_sort	Huixing, Liang
title	Influences of 1,2-dichlorobenzene on bacterial community structure in wetland soil
title_short	Influences of 1,2-dichlorobenzene on bacterial community structure in wetland soil
title_full	Influences of 1,2-dichlorobenzene on bacterial community structure in wetland soil
title_fullStr	Influences of 1,2-dichlorobenzene on bacterial community structure in wetland soil
title_full_unstemmed	Influences of 1,2-dichlorobenzene on bacterial community structure in wetland soil
title_sort	influences of 1,2-dichlorobenzene on bacterial community structure in wetland soil
publisher	Universiti Kebangsaan Malaysia
publishDate	2016
url	http://journalarticle.ukm.my/9578/ http://journalarticle.ukm.my/9578/ http://journalarticle.ukm.my/9578/1/16_Huixing_Liang.pdf
first_indexed	2023-09-18T19:55:17Z
last_indexed	2023-09-18T19:55:17Z
_version_	1777406487824433152

Influences of 1,2-dichlorobenzene on bacterial community structure in wetland soil

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