Evaluation of antioxidant properties, cytotoxicity and acute oral toxicity of Gynura procumbens (Compositae)

Gynura procumbens which is locally known as ‘Sambung nyawa’ in Malay and ‘Feng Wei Jian’ in Chinese, belongs to the botanical family of Compositae. In this study, antioxidant property of G. procumbens extracts was evaluated using DPPH radical scavenging, metal chelating and β-carotene bleaching as...

Full description

Bibliographic Details
Main Authors:	Wuen, Yew Teoh, Norhanom Abdul Wahab, Richardson, Jaime Stella Mose, Kae, Shin Sim
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2016
Online Access:	http://journalarticle.ukm.my/9677/ http://journalarticle.ukm.my/9677/ http://journalarticle.ukm.my/9677/1/10_Wuen_Yew_Teoh.pdf

id	ukm-9677
recordtype	eprints
spelling	ukm-96772016-12-14T06:50:31Z http://journalarticle.ukm.my/9677/ Evaluation of antioxidant properties, cytotoxicity and acute oral toxicity of Gynura procumbens (Compositae) Wuen, Yew Teoh Norhanom Abdul Wahab, Richardson, Jaime Stella Mose Kae, Shin Sim Gynura procumbens which is locally known as ‘Sambung nyawa’ in Malay and ‘Feng Wei Jian’ in Chinese, belongs to the botanical family of Compositae. In this study, antioxidant property of G. procumbens extracts was evaluated using DPPH radical scavenging, metal chelating and β-carotene bleaching assays. To the best of our knowledge, this is the first report that evaluated the cytotoxicity of G. procumbens extracts on human colon cancer cells (HT-29, HCT 116, HCT-15, SW480, Caco-2) and human normal colon cells (CCD-18Co). The results showed that ethyl acetate extract contained the highest total phenolic content (172.68 mg of GAEs/g of extract) compared to methanol, hexane and water extracts. Methanol extract possessed better overall antioxidant activities while ethyl acetate extract demonstrated better cytotoxic activity. At 24 h treatment, ethyl acetate extract demonstrated selective cytotoxicity against HT-29 and HCT 116 cells with IC50 values of 35.7 and 42.6 µg/mL, respectively. In addition, methanol extract showed negligible level of toxicity when administered orally. All the results indicated that G. procumbens may provide benefits in prevention and treatment of cancer. Universiti Kebangsaan Malaysia 2016-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9677/1/10_Wuen_Yew_Teoh.pdf Wuen, Yew Teoh and Norhanom Abdul Wahab, and Richardson, Jaime Stella Mose and Kae, Shin Sim (2016) Evaluation of antioxidant properties, cytotoxicity and acute oral toxicity of Gynura procumbens (Compositae). Sains Malaysiana, 45 (2). pp. 229-235. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid45bil2_2016/KandunganJilid45Bil2_2016.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Gynura procumbens which is locally known as ‘Sambung nyawa’ in Malay and ‘Feng Wei Jian’ in Chinese, belongs to the botanical family of Compositae. In this study, antioxidant property of G. procumbens extracts was evaluated using DPPH radical scavenging, metal chelating and β-carotene bleaching assays. To the best of our knowledge, this is the first report that evaluated the cytotoxicity of G. procumbens extracts on human colon cancer cells (HT-29, HCT 116, HCT-15, SW480, Caco-2) and human normal colon cells (CCD-18Co). The results showed that ethyl acetate extract contained the highest total phenolic content (172.68 mg of GAEs/g of extract) compared to methanol, hexane and water extracts. Methanol extract possessed better overall antioxidant activities while ethyl acetate extract demonstrated better cytotoxic activity. At 24 h treatment, ethyl acetate extract demonstrated selective cytotoxicity against HT-29 and HCT 116 cells with IC50 values of 35.7 and 42.6 µg/mL, respectively. In addition, methanol extract showed negligible level of toxicity when administered orally. All the results indicated that G. procumbens may provide benefits in prevention and treatment of cancer.
format	Article
author	Wuen, Yew Teoh Norhanom Abdul Wahab, Richardson, Jaime Stella Mose Kae, Shin Sim
spellingShingle	Wuen, Yew Teoh Norhanom Abdul Wahab, Richardson, Jaime Stella Mose Kae, Shin Sim Evaluation of antioxidant properties, cytotoxicity and acute oral toxicity of Gynura procumbens (Compositae)
author_facet	Wuen, Yew Teoh Norhanom Abdul Wahab, Richardson, Jaime Stella Mose Kae, Shin Sim
author_sort	Wuen, Yew Teoh
title	Evaluation of antioxidant properties, cytotoxicity and acute oral toxicity of Gynura procumbens (Compositae)
title_short	Evaluation of antioxidant properties, cytotoxicity and acute oral toxicity of Gynura procumbens (Compositae)
title_full	Evaluation of antioxidant properties, cytotoxicity and acute oral toxicity of Gynura procumbens (Compositae)
title_fullStr	Evaluation of antioxidant properties, cytotoxicity and acute oral toxicity of Gynura procumbens (Compositae)
title_full_unstemmed	Evaluation of antioxidant properties, cytotoxicity and acute oral toxicity of Gynura procumbens (Compositae)
title_sort	evaluation of antioxidant properties, cytotoxicity and acute oral toxicity of gynura procumbens (compositae)
publisher	Universiti Kebangsaan Malaysia
publishDate	2016
url	http://journalarticle.ukm.my/9677/ http://journalarticle.ukm.my/9677/ http://journalarticle.ukm.my/9677/1/10_Wuen_Yew_Teoh.pdf
first_indexed	2023-09-18T19:55:24Z
last_indexed	2023-09-18T19:55:24Z
_version_	1777406495096307712

Evaluation of antioxidant properties, cytotoxicity and acute oral toxicity of Gynura procumbens (Compositae)

Similar Items