Significant replication time-points of avian influenza A virus strain H5N1 in Madin-Darby Canine Kidney cells

The occasional influenza pandemics and the seasonal influenza epidemics have destroyed millions of lives since the last century. It is therefore necessary to understand the virus replication patterns as this provides essential information on the virus infectivity, pathogenicity and spread patterns...

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Main Authors:	Toong, Seng Tan, Sharifah Syed Hassan, Wei, Boon Yap
Format:	Article
Language:	English
Published:	Penerbit UKM 2016
Online Access:	http://journalarticle.ukm.my/9725/ http://journalarticle.ukm.my/9725/ http://journalarticle.ukm.my/9725/1/12265-33453-2-PB.pdf

id	ukm-9725
recordtype	eprints
spelling	ukm-97252016-12-14T06:50:39Z http://journalarticle.ukm.my/9725/ Significant replication time-points of avian influenza A virus strain H5N1 in Madin-Darby Canine Kidney cells Toong, Seng Tan Sharifah Syed Hassan, Wei, Boon Yap The occasional influenza pandemics and the seasonal influenza epidemics have destroyed millions of lives since the last century. It is therefore necessary to understand the virus replication patterns as this provides essential information on the virus infectivity, pathogenicity and spread patterns. This study aimed to investigate the replication of avian influenza A virus H5N1 (A/Chicken/Malaysia/5858/2004) in MDCK cells. In this study, the TCID50 (50% tissue culture infectious dose) of AIV H5N1 was first determined. The MDCK cells were then infected with AIV H5N1 at TCID50 for 0-48 h. The CPE (cytopathic effect) was observed and cell death was determined hourly. The virus-infected cells and media were subsequently collected for gene analysis. The results showed that the TCID50 of AIV H5N1 was 10-9 dilution. The CPE percentage showed a strong and positive correlation with the infection period (r = 1.0, n = 9, p < 0.01). The amount of a highly conserved influenza viral gene, M2 gene amplified from infected media (r = 0.471, n = 9, p= > 0.05) and infected cell (r = 0.73, n = 9, p < 0.05) were also positively correlated with the infection period. In conclusion, although CPE started to be observed in the early time points of infection, however, the M2 gene was only amplified from the infected media and cells after 48 h and 24 h, respectively. This signifies that AIV H5N1 used in this study is pathogenic and it is able to cause severe cytopathology to host cells even at low virus load. Penerbit UKM 2016 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9725/1/12265-33453-2-PB.pdf Toong, Seng Tan and Sharifah Syed Hassan, and Wei, Boon Yap (2016) Significant replication time-points of avian influenza A virus strain H5N1 in Madin-Darby Canine Kidney cells. Jurnal Sains Kesihatan Malaysia, 14 (1). pp. 17-21. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/633
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The occasional influenza pandemics and the seasonal influenza epidemics have destroyed millions of lives since the last century. It is therefore necessary to understand the virus replication patterns as this provides essential information on the virus infectivity, pathogenicity and spread patterns. This study aimed to investigate the replication of avian influenza A virus H5N1 (A/Chicken/Malaysia/5858/2004) in MDCK cells. In this study, the TCID50 (50% tissue culture infectious dose) of AIV H5N1 was first determined. The MDCK cells were then infected with AIV H5N1 at TCID50 for 0-48 h. The CPE (cytopathic effect) was observed and cell death was determined hourly. The virus-infected cells and media were subsequently collected for gene analysis. The results showed that the TCID50 of AIV H5N1 was 10-9 dilution. The CPE percentage showed a strong and positive correlation with the infection period (r = 1.0, n = 9, p < 0.01). The amount of a highly conserved influenza viral gene, M2 gene amplified from infected media (r = 0.471, n = 9, p= > 0.05) and infected cell (r = 0.73, n = 9, p < 0.05) were also positively correlated with the infection period. In conclusion, although CPE started to be observed in the early time points of infection, however, the M2 gene was only amplified from the infected media and cells after 48 h and 24 h, respectively. This signifies that AIV H5N1 used in this study is pathogenic and it is able to cause severe cytopathology to host cells even at low virus load.
format	Article
author	Toong, Seng Tan Sharifah Syed Hassan, Wei, Boon Yap
spellingShingle	Toong, Seng Tan Sharifah Syed Hassan, Wei, Boon Yap Significant replication time-points of avian influenza A virus strain H5N1 in Madin-Darby Canine Kidney cells
author_facet	Toong, Seng Tan Sharifah Syed Hassan, Wei, Boon Yap
author_sort	Toong, Seng Tan
title	Significant replication time-points of avian influenza A virus strain H5N1 in Madin-Darby Canine Kidney cells
title_short	Significant replication time-points of avian influenza A virus strain H5N1 in Madin-Darby Canine Kidney cells
title_full	Significant replication time-points of avian influenza A virus strain H5N1 in Madin-Darby Canine Kidney cells
title_fullStr	Significant replication time-points of avian influenza A virus strain H5N1 in Madin-Darby Canine Kidney cells
title_full_unstemmed	Significant replication time-points of avian influenza A virus strain H5N1 in Madin-Darby Canine Kidney cells
title_sort	significant replication time-points of avian influenza a virus strain h5n1 in madin-darby canine kidney cells
publisher	Penerbit UKM
publishDate	2016
url	http://journalarticle.ukm.my/9725/ http://journalarticle.ukm.my/9725/ http://journalarticle.ukm.my/9725/1/12265-33453-2-PB.pdf
first_indexed	2023-09-18T19:55:32Z
last_indexed	2023-09-18T19:55:32Z
_version_	1777406504023883776

Significant replication time-points of avian influenza A virus strain H5N1 in Madin-Darby Canine Kidney cells

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