Assessment of DNA strand breakage in streptozotocin-induced diabetic Rats

This study was conducted to evaluate the oxidative damage in diabetic mellitus induced rats. The evaluation of DNA damage was carried out by the Alkaline Comet Assay using peripheral lymphocyte cells taken from streptozotocin-induced diabetic rats (50 mg/kg) and control rats. The levels of malondea...

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Main Authors:	Siti Balkis Budin, Norfadilah Rejab, Abdul Gapor Mohd Top, Wan Nozaimoon Wan Mohamud, Mokhtar Abu Bakar, Khairul Osman, Jamaludin Mohamed
Format:	Article
Language:	English
Published:	penerbit ukm 2005
Online Access:	http://journalarticle.ukm.my/982/ http://journalarticle.ukm.my/982/ http://journalarticle.ukm.my/982/1/jurnal35.pdf

id	ukm-982
recordtype	eprints
spelling	ukm-9822016-12-14T06:28:29Z http://journalarticle.ukm.my/982/ Assessment of DNA strand breakage in streptozotocin-induced diabetic Rats Siti Balkis Budin, Norfadilah Rejab, Abdul Gapor Mohd Top, Wan Nozaimoon Wan Mohamud, Mokhtar Abu Bakar, Khairul Osman, Jamaludin Mohamed, This study was conducted to evaluate the oxidative damage in diabetic mellitus induced rats. The evaluation of DNA damage was carried out by the Alkaline Comet Assay using peripheral lymphocyte cells taken from streptozotocin-induced diabetic rats (50 mg/kg) and control rats. The levels of malondealdehyde (MDA), 4-hydroxynonenal (4-HNE), fasting blood glucose (FBG) and HbA1c were also measured. All the induced diabetic rats were hyperglycemic until the end of the study with significantly higher levels of FBG and HbA1c as compared to the control rats. The results showed the percentage of tail DNA and tail moment values were also significantly higher in the diabetic induced rats. The same observations were made on the levels of plasma MDA and 4-HNE. In conclusion, this study indicated that hyperglycemic condition in diabetic induced rats could generate oxidative DNA damage. penerbit ukm 2005 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/982/1/jurnal35.pdf Siti Balkis Budin, and Norfadilah Rejab, and Abdul Gapor Mohd Top, and Wan Nozaimoon Wan Mohamud, and Mokhtar Abu Bakar, and Khairul Osman, and Jamaludin Mohamed, (2005) Assessment of DNA strand breakage in streptozotocin-induced diabetic Rats. Jurnal Sains Kesihatan Malaysia, 3 (1). pp. 1-12. ISSN 1675-8161 http://www.fskb.ukm.my/fskbold/jurnal
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	This study was conducted to evaluate the oxidative damage in diabetic mellitus induced rats. The evaluation of DNA damage was carried out by the Alkaline Comet Assay using peripheral lymphocyte cells taken from streptozotocin-induced diabetic rats (50 mg/kg) and control rats. The levels of malondealdehyde (MDA), 4-hydroxynonenal (4-HNE), fasting blood glucose (FBG) and HbA1c were also measured. All the induced diabetic rats were hyperglycemic until the end of the study with significantly higher levels of FBG and HbA1c as compared to the control rats. The results showed the percentage of tail DNA and tail moment values were also significantly higher in the diabetic induced rats. The same observations were made on the levels of plasma MDA and 4-HNE. In conclusion, this study indicated that hyperglycemic condition in diabetic induced rats could generate oxidative DNA damage.
format	Article
author	Siti Balkis Budin, Norfadilah Rejab, Abdul Gapor Mohd Top, Wan Nozaimoon Wan Mohamud, Mokhtar Abu Bakar, Khairul Osman, Jamaludin Mohamed,
spellingShingle	Siti Balkis Budin, Norfadilah Rejab, Abdul Gapor Mohd Top, Wan Nozaimoon Wan Mohamud, Mokhtar Abu Bakar, Khairul Osman, Jamaludin Mohamed, Assessment of DNA strand breakage in streptozotocin-induced diabetic Rats
author_facet	Siti Balkis Budin, Norfadilah Rejab, Abdul Gapor Mohd Top, Wan Nozaimoon Wan Mohamud, Mokhtar Abu Bakar, Khairul Osman, Jamaludin Mohamed,
author_sort	Siti Balkis Budin,
title	Assessment of DNA strand breakage in streptozotocin-induced diabetic Rats
title_short	Assessment of DNA strand breakage in streptozotocin-induced diabetic Rats
title_full	Assessment of DNA strand breakage in streptozotocin-induced diabetic Rats
title_fullStr	Assessment of DNA strand breakage in streptozotocin-induced diabetic Rats
title_full_unstemmed	Assessment of DNA strand breakage in streptozotocin-induced diabetic Rats
title_sort	assessment of dna strand breakage in streptozotocin-induced diabetic rats
publisher	penerbit ukm
publishDate	2005
url	http://journalarticle.ukm.my/982/ http://journalarticle.ukm.my/982/ http://journalarticle.ukm.my/982/1/jurnal35.pdf
first_indexed	2023-09-18T19:32:03Z
last_indexed	2023-09-18T19:32:03Z
_version_	1777405026616999936

Assessment of DNA strand breakage in streptozotocin-induced diabetic Rats

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