Isolation and characterization of full-length cellulose synthase gene (HsCesA1) from Roselle (Hibiscus sabdariffa L. var. UMKL)

The Hibiscus sabdariffa var. UMKL (Roselle) investigated here may potentially be used as an alternative fibre source. To the best of our knowledge, there was no study focusing on the genetics underlying the cellulose biosynthesis machinery in Roselle thus far. This paper presents the results of the...

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Main Authors:	Seyedi, Seyedeh Sareh, Soon, Guan Tan, Parameswari Namasivayam, Seok, Christina Yien Yong
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2016
Online Access:	http://journalarticle.ukm.my/9874/ http://journalarticle.ukm.my/9874/ http://journalarticle.ukm.my/9874/1/07_Seyedeh.pdf

id	ukm-9874
recordtype	eprints
spelling	ukm-98742016-12-14T06:51:07Z http://journalarticle.ukm.my/9874/ Isolation and characterization of full-length cellulose synthase gene (HsCesA1) from Roselle (Hibiscus sabdariffa L. var. UMKL) Seyedi, Seyedeh Sareh Soon, Guan Tan Parameswari Namasivayam, Seok, Christina Yien Yong The Hibiscus sabdariffa var. UMKL (Roselle) investigated here may potentially be used as an alternative fibre source. To the best of our knowledge, there was no study focusing on the genetics underlying the cellulose biosynthesis machinery in Roselle thus far. This paper presents the results of the first isolation of the cellulose synthase gene, HsCesA1 from this plant, which is fundamental for working towards understanding the functions of CesA genes in the cellulose biosynthesis of Roselle. A full-length HsCesA1 cDNA of 3528 bp in length (accession no: KJ608192) encoding a polypeptide of 974 amino acid was isolated. The full-length HsCesA1 gene of 5489 bp length (accession no: KJ661223) with 11-introns and a promoter region of 737 bp was further isolated. Important and conserved characteristics of a CesA protein were identified in the HsCesA1 deduced amino acid sequence, which strengthened the prediction that the isolated gene being a cellulose synthase belonging to the processive class of the 2-glycosyltransferase family 2A. Relative gene expression analysis by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) on young leaf and stem tissues found that HsCesA1 had similar levels of gene expression in both tissues. Phylogenetic and Blast analyses also supported the prediction that the isolated HsCesA1 may play roles in the cell wall depositions in both leaf and stem tissues. Penerbit Universiti Kebangsaan Malaysia 2016-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9874/1/07_Seyedeh.pdf Seyedi, Seyedeh Sareh and Soon, Guan Tan and Parameswari Namasivayam, and Seok, Christina Yien Yong (2016) Isolation and characterization of full-length cellulose synthase gene (HsCesA1) from Roselle (Hibiscus sabdariffa L. var. UMKL). Sains Malaysiana, 45 (5). pp. 717-727. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid45bil5_2016/KandunganJilid45Bil5_2016.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The Hibiscus sabdariffa var. UMKL (Roselle) investigated here may potentially be used as an alternative fibre source. To the best of our knowledge, there was no study focusing on the genetics underlying the cellulose biosynthesis machinery in Roselle thus far. This paper presents the results of the first isolation of the cellulose synthase gene, HsCesA1 from this plant, which is fundamental for working towards understanding the functions of CesA genes in the cellulose biosynthesis of Roselle. A full-length HsCesA1 cDNA of 3528 bp in length (accession no: KJ608192) encoding a polypeptide of 974 amino acid was isolated. The full-length HsCesA1 gene of 5489 bp length (accession no: KJ661223) with 11-introns and a promoter region of 737 bp was further isolated. Important and conserved characteristics of a CesA protein were identified in the HsCesA1 deduced amino acid sequence, which strengthened the prediction that the isolated gene being a cellulose synthase belonging to the processive class of the 2-glycosyltransferase family 2A. Relative gene expression analysis by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) on young leaf and stem tissues found that HsCesA1 had similar levels of gene expression in both tissues. Phylogenetic and Blast analyses also supported the prediction that the isolated HsCesA1 may play roles in the cell wall depositions in both leaf and stem tissues.
format	Article
author	Seyedi, Seyedeh Sareh Soon, Guan Tan Parameswari Namasivayam, Seok, Christina Yien Yong
spellingShingle	Seyedi, Seyedeh Sareh Soon, Guan Tan Parameswari Namasivayam, Seok, Christina Yien Yong Isolation and characterization of full-length cellulose synthase gene (HsCesA1) from Roselle (Hibiscus sabdariffa L. var. UMKL)
author_facet	Seyedi, Seyedeh Sareh Soon, Guan Tan Parameswari Namasivayam, Seok, Christina Yien Yong
author_sort	Seyedi, Seyedeh Sareh
title	Isolation and characterization of full-length cellulose synthase gene (HsCesA1) from Roselle (Hibiscus sabdariffa L. var. UMKL)
title_short	Isolation and characterization of full-length cellulose synthase gene (HsCesA1) from Roselle (Hibiscus sabdariffa L. var. UMKL)
title_full	Isolation and characterization of full-length cellulose synthase gene (HsCesA1) from Roselle (Hibiscus sabdariffa L. var. UMKL)
title_fullStr	Isolation and characterization of full-length cellulose synthase gene (HsCesA1) from Roselle (Hibiscus sabdariffa L. var. UMKL)
title_full_unstemmed	Isolation and characterization of full-length cellulose synthase gene (HsCesA1) from Roselle (Hibiscus sabdariffa L. var. UMKL)
title_sort	isolation and characterization of full-length cellulose synthase gene (hscesa1) from roselle (hibiscus sabdariffa l. var. umkl)
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2016
url	http://journalarticle.ukm.my/9874/ http://journalarticle.ukm.my/9874/ http://journalarticle.ukm.my/9874/1/07_Seyedeh.pdf
first_indexed	2023-09-18T19:55:58Z
last_indexed	2023-09-18T19:55:58Z
_version_	1777406531235479552

Isolation and characterization of full-length cellulose synthase gene (HsCesA1) from Roselle (Hibiscus sabdariffa L. var. UMKL)

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