Phylogenetic relationships within the Genus Holothuria inferred from 16S Mitochondiral rRNA Gene Sequences

This study aimed to resolve the taxonomic status of a morphologically undetermined sea cucumber species of order Apodida from Malaysia (GenBank accession no.: FJ223867) using partial 16S mitochondrial rRNA gene sequences and subsequently to determine the validity of morphological taxonomy of Holothu...

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Main Authors:	Kamarul Rahim Kamarudin, Aisyah Mohamed Rehan, Ridzwan Hashim, Gires Usup, Maryam Mohamed Rehan
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2016
Online Access:	http://journalarticle.ukm.my/9982/ http://journalarticle.ukm.my/9982/ http://journalarticle.ukm.my/9982/1/10%20Kamarul%20Rahim%20Kamarudin.pdf

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spelling	ukm-99822017-01-19T09:30:56Z http://journalarticle.ukm.my/9982/ Phylogenetic relationships within the Genus Holothuria inferred from 16S Mitochondiral rRNA Gene Sequences Kamarul Rahim Kamarudin, Aisyah Mohamed Rehan, Ridzwan Hashim, Gires Usup, Maryam Mohamed Rehan, This study aimed to resolve the taxonomic status of a morphologically undetermined sea cucumber species of order Apodida from Malaysia (GenBank accession no.: FJ223867) using partial 16S mitochondrial rRNA gene sequences and subsequently to determine the validity of morphological taxonomy of Holothuria species into its current subgenera. The undetermined species clustered with all taxa of Holothuria in previous study. Phylogenetic analyses using maximum parsimony and Bayesian methods suggest that the undetermined species was genetically closer to Holothuria (Lessonothuria) pardalis and Holothuria (Acanthotrapeza) coluber; and its position in both phylogenetic trees further suggests its status as a Holothuria taxon. Subgenera of Holothuria, Merthensiothuria and Metriatyla are monophyletic with strong bootstrap supports and posterior probabilities of clades, thus strengthening their morphological taxonomies. Nonetheless, the non-monophyly of subgenera of Halodeima, Microthele and Platyperona suggests a requirement for their taxonomic revisions using integrative taxonomy. The status of Holothuria (Halodeima) edulis subgroups in the maximum parsimony and Bayesian trees is indistinct and further taxonomic revisions are necessary. In terms of sister relationship, both phylogenetic trees suggest that subgenus Holothuria is a sister taxon of subgenus Roweothuria while the other sister relationships were unclear due to the undetermined species, paraphyly and polyphyly of a number of subgenera. Further studies with more specimens of genus Holothuria from broader geographical locations and various mtDNA genes along with morphological approaches may facilitate to provide better insights into the molecular phylogeny of subgenera of Holothuria. Penerbit Universiti Kebangsaan Malaysia 2016-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9982/1/10%20Kamarul%20Rahim%20Kamarudin.pdf Kamarul Rahim Kamarudin, and Aisyah Mohamed Rehan, and Ridzwan Hashim, and Gires Usup, and Maryam Mohamed Rehan, (2016) Phylogenetic relationships within the Genus Holothuria inferred from 16S Mitochondiral rRNA Gene Sequences. Sains Malaysiana, 45 (7). pp. 1079-1087. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol45num7_2016/contentsVol45num7_2016.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	This study aimed to resolve the taxonomic status of a morphologically undetermined sea cucumber species of order Apodida from Malaysia (GenBank accession no.: FJ223867) using partial 16S mitochondrial rRNA gene sequences and subsequently to determine the validity of morphological taxonomy of Holothuria species into its current subgenera. The undetermined species clustered with all taxa of Holothuria in previous study. Phylogenetic analyses using maximum parsimony and Bayesian methods suggest that the undetermined species was genetically closer to Holothuria (Lessonothuria) pardalis and Holothuria (Acanthotrapeza) coluber; and its position in both phylogenetic trees further suggests its status as a Holothuria taxon. Subgenera of Holothuria, Merthensiothuria and Metriatyla are monophyletic with strong bootstrap supports and posterior probabilities of clades, thus strengthening their morphological taxonomies. Nonetheless, the non-monophyly of subgenera of Halodeima, Microthele and Platyperona suggests a requirement for their taxonomic revisions using integrative taxonomy. The status of Holothuria (Halodeima) edulis subgroups in the maximum parsimony and Bayesian trees is indistinct and further taxonomic revisions are necessary. In terms of sister relationship, both phylogenetic trees suggest that subgenus Holothuria is a sister taxon of subgenus Roweothuria while the other sister relationships were unclear due to the undetermined species, paraphyly and polyphyly of a number of subgenera. Further studies with more specimens of genus Holothuria from broader geographical locations and various mtDNA genes along with morphological approaches may facilitate to provide better insights into the molecular phylogeny of subgenera of Holothuria.
format	Article
author	Kamarul Rahim Kamarudin, Aisyah Mohamed Rehan, Ridzwan Hashim, Gires Usup, Maryam Mohamed Rehan,
spellingShingle	Kamarul Rahim Kamarudin, Aisyah Mohamed Rehan, Ridzwan Hashim, Gires Usup, Maryam Mohamed Rehan, Phylogenetic relationships within the Genus Holothuria inferred from 16S Mitochondiral rRNA Gene Sequences
author_facet	Kamarul Rahim Kamarudin, Aisyah Mohamed Rehan, Ridzwan Hashim, Gires Usup, Maryam Mohamed Rehan,
author_sort	Kamarul Rahim Kamarudin,
title	Phylogenetic relationships within the Genus Holothuria inferred from 16S Mitochondiral rRNA Gene Sequences
title_short	Phylogenetic relationships within the Genus Holothuria inferred from 16S Mitochondiral rRNA Gene Sequences
title_full	Phylogenetic relationships within the Genus Holothuria inferred from 16S Mitochondiral rRNA Gene Sequences
title_fullStr	Phylogenetic relationships within the Genus Holothuria inferred from 16S Mitochondiral rRNA Gene Sequences
title_full_unstemmed	Phylogenetic relationships within the Genus Holothuria inferred from 16S Mitochondiral rRNA Gene Sequences
title_sort	phylogenetic relationships within the genus holothuria inferred from 16s mitochondiral rrna gene sequences
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2016
url	http://journalarticle.ukm.my/9982/ http://journalarticle.ukm.my/9982/ http://journalarticle.ukm.my/9982/1/10%20Kamarul%20Rahim%20Kamarudin.pdf
first_indexed	2023-09-18T19:56:17Z
last_indexed	2023-09-18T19:56:17Z
_version_	1777406550785130496

Phylogenetic relationships within the Genus Holothuria inferred from 16S Mitochondiral rRNA Gene Sequences

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