Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor
Mercury is an extremely toxic pollutant that currently being emitted and distributed globally. Several petroleum based industrial plants had showed high concentration of mercury compare to the Department of Environmental (DOE), Malaysia. Microbes have been used to solve environmental wastewater pr...
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ump-107042020-02-28T02:22:09Z http://umpir.ump.edu.my/id/eprint/10704/ Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor Abdul Aziz, Mohd Azoddein R. A., Bakar Nik Meriam, Nik Sulaiman Ahmad Bazli, Bustary Nur Athirah, Mohamad Basir TP Chemical technology Mercury is an extremely toxic pollutant that currently being emitted and distributed globally. Several petroleum based industrial plants had showed high concentration of mercury compare to the Department of Environmental (DOE), Malaysia. Microbes have been used to solve environmental wastewater problems for many years. Objective of this study is to remove mercury from actual petrochemical wastewater using Pseudomonas putida (P. putida), in membrane bioreactor. To achieve the maximum of mercury removal, the optimum growth parameters of P. putida were obtained. Based on the optimum parameters of P. putida for specific growth rate, µ the removal of 4 mg/L was studied. Results showed mercury removal for sample with 4 mg/L mercury in bioreactor is 99.60% for the first 6 hours, 99.80% removal for 120 hours and 99.90 % after the microfiltration membrane system. The specific growth rate (μ) describes how fast the cells are reproducing. The higher the value of specific growth rate, then the faster the cells are growing.In this case, 6 hours was the optimum time for the mercury removal with the ratio of mercury mass over cell mass is 20.78 µgHg/g cells for P. putida. Microfiltration membrane enhanced further the treatment of the wastewater by retaining the P. putida from escaping during the release of treated wastewater, reducing the turbidity by 94.2% (5.32 NTU) and concentration of suspended solids up to 60.4% (0.09 mg/L). The mechanism of mercury detoxification in the membrane bioreactor was based on reduction of Hg²⁺ to non-toxic Hg0 by mercury reductase enzyme produced by P. putida. The findings from this study can be used as references for future application of petroleum based industries wastewater treatment as well as other industries related to mercury contamination in their wastewater treatment plant such as gold mining, chemical industries, agriculture etc. 2015 Conference or Workshop Item PeerReviewed application/pdf en cc_by_nc_nd http://umpir.ump.edu.my/id/eprint/10704/1/Mercury%20Removal%20from%20Actual%20Petroleum%20Based%20Industries%20Wastewater%20by%20P.%20putida%20in%20Membrane%20Bioreactor.pdf Abdul Aziz, Mohd Azoddein and R. A., Bakar and Nik Meriam, Nik Sulaiman and Ahmad Bazli, Bustary and Nur Athirah, Mohamad Basir (2015) Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor. In: New Directions in Multidisciplinary Research & Practice (NDMRP 2015), 12-13 May 2015 , Istanbul, Turkey. pp. 313-330.. http://www.globalilluminators.org/ndmrp-2015-turkey/full-paper-proceding-ndmrp-2015-turkey/ |
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TP Chemical technology Abdul Aziz, Mohd Azoddein R. A., Bakar Nik Meriam, Nik Sulaiman Ahmad Bazli, Bustary Nur Athirah, Mohamad Basir Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor |
description |
Mercury is an extremely toxic pollutant that currently being emitted and distributed globally. Several petroleum
based industrial plants had showed high concentration of mercury compare to the Department of Environmental
(DOE), Malaysia. Microbes have been used to solve environmental wastewater problems for many years.
Objective of this study is to remove mercury from actual petrochemical wastewater using Pseudomonas putida
(P. putida), in membrane bioreactor. To achieve the maximum of mercury removal, the optimum growth
parameters of P. putida were obtained. Based on the optimum parameters of P. putida for specific growth rate, µ
the removal of 4 mg/L was studied. Results showed mercury removal for sample with 4 mg/L mercury in
bioreactor is 99.60% for the first 6 hours, 99.80% removal for 120 hours and 99.90 % after the microfiltration
membrane system. The specific growth rate (μ) describes how fast the cells are reproducing. The higher the
value of specific growth rate, then the faster the cells are growing.In this case, 6 hours was the optimum time for
the mercury removal with the ratio of mercury mass over cell mass is 20.78 µgHg/g cells for P. putida.
Microfiltration membrane enhanced further the treatment of the wastewater by retaining the P. putida from
escaping during the release of treated wastewater, reducing the turbidity by 94.2% (5.32 NTU) and
concentration of suspended solids up to 60.4% (0.09 mg/L). The mechanism of mercury detoxification in the
membrane bioreactor was based on reduction of Hg²⁺ to non-toxic Hg0 by mercury reductase enzyme produced
by P. putida. The findings from this study can be used as references for future application of petroleum based
industries wastewater treatment as well as other industries related to mercury contamination in their wastewater
treatment plant such as gold mining, chemical industries, agriculture etc. |
format |
Conference or Workshop Item |
author |
Abdul Aziz, Mohd Azoddein R. A., Bakar Nik Meriam, Nik Sulaiman Ahmad Bazli, Bustary Nur Athirah, Mohamad Basir |
author_facet |
Abdul Aziz, Mohd Azoddein R. A., Bakar Nik Meriam, Nik Sulaiman Ahmad Bazli, Bustary Nur Athirah, Mohamad Basir |
author_sort |
Abdul Aziz, Mohd Azoddein |
title |
Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor |
title_short |
Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor |
title_full |
Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor |
title_fullStr |
Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor |
title_full_unstemmed |
Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor |
title_sort |
mercury removal from actual petroleum based industries wastewater by p. putida in membrane bioreactor |
publishDate |
2015 |
url |
http://umpir.ump.edu.my/id/eprint/10704/ http://umpir.ump.edu.my/id/eprint/10704/ http://umpir.ump.edu.my/id/eprint/10704/1/Mercury%20Removal%20from%20Actual%20Petroleum%20Based%20Industries%20Wastewater%20by%20P.%20putida%20in%20Membrane%20Bioreactor.pdf |
first_indexed |
2023-09-18T22:10:36Z |
last_indexed |
2023-09-18T22:10:36Z |
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