Fabrication of glucose sensor using graphene

Graphene is a one-atom-thick allotrope of carbon. Due to its unique mechanical and electronic properties, graphene has been touted as very promising material for a great number of applications. In particular, biosensor is a device for the detection of specific analyte which combines a biological com...

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Main Author:	Soo Ling, Chong
Format:	Undergraduates Project Papers
Language:	English English English
Published:	2015
Subjects:	QD Chemistry
Online Access:	http://umpir.ump.edu.my/id/eprint/11011/ http://umpir.ump.edu.my/id/eprint/11011/ http://umpir.ump.edu.my/id/eprint/11011/1/FKKSA%20-%20CHONG%20SOO%20LIANG%20%28CD8979%29.pdf http://umpir.ump.edu.my/id/eprint/11011/2/FKKSA%20-%20CHONG%20SOO%20LIANG%20%28CD8979%29%20CHAP%201.pdf http://umpir.ump.edu.my/id/eprint/11011/6/FKKSA%20-%20CHONG%20SOO%20LIANG%20%28CD8979%29%20CHAP%203.pdf

id	ump-11011
recordtype	eprints
spelling	ump-110112015-11-02T01:10:20Z http://umpir.ump.edu.my/id/eprint/11011/ Fabrication of glucose sensor using graphene Soo Ling, Chong QD Chemistry Graphene is a one-atom-thick allotrope of carbon. Due to its unique mechanical and electronic properties, graphene has been touted as very promising material for a great number of applications. In particular, biosensor is a device for the detection of specific analyte which combines a biological compound with a physicochemical detector component. There are three major components that complete a biosensor, namely the sensitive biological element, the transducer or detector element and also the associated electronics or signal processors. The working principle of graphene-based enzymatic electrodes is based on the direct electrochemistry of enzymes whereby electron transfer occurs between the electrode and the active centre of the enzymes without the participation of any mediators or reagents. In the current work, a graphene based glucose biosensor was prepared. The reduced graphene oxide (RGO)-glucose oxidase (GOx) was prepared from graphite powder as starting material. Material synthesis involved pre-oxidation of graphite to graphite oxide followed by further oxidation to graphene oxide. Subsequently, hydrazine monohydrate was used to reduce the graphene oxide to reduced graphene oxide. Finally, glassy carbon electrode (GCE) /RGO-GOx based glucose sensor was fabricated. Thereafter, the sample was tested with different concentrations of glucose solution. It was also being subjected to ultraviolet-visible absorption spectrophotometry (UV-Vis), Fourier transform infrared spectroscopy (FTIR) and cyclic voltammetry (CV). From this study, the obtained results are allowed for assessment of an optimum and sensitivity of the GCE/RGO-GOx based glucose biosensor towards the glucose concentration. It can be concluded that GCE/RGO-GOx based glucose biosensor can detect circa 3 to 7 mM glucose. 2015-01 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/11011/1/FKKSA%20-%20CHONG%20SOO%20LIANG%20%28CD8979%29.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/11011/2/FKKSA%20-%20CHONG%20SOO%20LIANG%20%28CD8979%29%20CHAP%201.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/11011/6/FKKSA%20-%20CHONG%20SOO%20LIANG%20%28CD8979%29%20CHAP%203.pdf Soo Ling, Chong (2015) Fabrication of glucose sensor using graphene. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:91270&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English English
topic	QD Chemistry
spellingShingle	QD Chemistry Soo Ling, Chong Fabrication of glucose sensor using graphene
description	Graphene is a one-atom-thick allotrope of carbon. Due to its unique mechanical and electronic properties, graphene has been touted as very promising material for a great number of applications. In particular, biosensor is a device for the detection of specific analyte which combines a biological compound with a physicochemical detector component. There are three major components that complete a biosensor, namely the sensitive biological element, the transducer or detector element and also the associated electronics or signal processors. The working principle of graphene-based enzymatic electrodes is based on the direct electrochemistry of enzymes whereby electron transfer occurs between the electrode and the active centre of the enzymes without the participation of any mediators or reagents. In the current work, a graphene based glucose biosensor was prepared. The reduced graphene oxide (RGO)-glucose oxidase (GOx) was prepared from graphite powder as starting material. Material synthesis involved pre-oxidation of graphite to graphite oxide followed by further oxidation to graphene oxide. Subsequently, hydrazine monohydrate was used to reduce the graphene oxide to reduced graphene oxide. Finally, glassy carbon electrode (GCE) /RGO-GOx based glucose sensor was fabricated. Thereafter, the sample was tested with different concentrations of glucose solution. It was also being subjected to ultraviolet-visible absorption spectrophotometry (UV-Vis), Fourier transform infrared spectroscopy (FTIR) and cyclic voltammetry (CV). From this study, the obtained results are allowed for assessment of an optimum and sensitivity of the GCE/RGO-GOx based glucose biosensor towards the glucose concentration. It can be concluded that GCE/RGO-GOx based glucose biosensor can detect circa 3 to 7 mM glucose.
format	Undergraduates Project Papers
author	Soo Ling, Chong
author_facet	Soo Ling, Chong
author_sort	Soo Ling, Chong
title	Fabrication of glucose sensor using graphene
title_short	Fabrication of glucose sensor using graphene
title_full	Fabrication of glucose sensor using graphene
title_fullStr	Fabrication of glucose sensor using graphene
title_full_unstemmed	Fabrication of glucose sensor using graphene
title_sort	fabrication of glucose sensor using graphene
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/11011/ http://umpir.ump.edu.my/id/eprint/11011/ http://umpir.ump.edu.my/id/eprint/11011/1/FKKSA%20-%20CHONG%20SOO%20LIANG%20%28CD8979%29.pdf http://umpir.ump.edu.my/id/eprint/11011/2/FKKSA%20-%20CHONG%20SOO%20LIANG%20%28CD8979%29%20CHAP%201.pdf http://umpir.ump.edu.my/id/eprint/11011/6/FKKSA%20-%20CHONG%20SOO%20LIANG%20%28CD8979%29%20CHAP%203.pdf
first_indexed	2023-09-18T22:11:17Z
last_indexed	2023-09-18T22:11:17Z
_version_	1777415044854710272

Fabrication of glucose sensor using graphene

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