Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil

Hyperlipidemia is a main risk factor for coronary heart disease and stroke. The lipid-lowering drug, pravastatin, is currently obtained by the microbial biotransformation of compactin (mevastatin) to pravastatin. It is clear that pharmaceutical industry has to date utilized only a very minor porti...

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Main Author:	Seydametova, Emine
Format:	Article
Language:	English English
Published:	International Academy Publishing (IAP) 2015
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/11594/ http://umpir.ump.edu.my/id/eprint/11594/ http://umpir.ump.edu.my/id/eprint/11594/ http://umpir.ump.edu.my/id/eprint/11594/1/Novel%20Pravastatin-Producing%20Penicillium%20janthinellum%20Strain%20Isolated%20from%20Soil.pdf http://umpir.ump.edu.my/id/eprint/11594/7/Novel%20Pravastatin-Producing%20Penicillium%20janthinellum%20Strain%20Isolated%20from%20Soil.pdf

id	ump-11594
recordtype	eprints
spelling	ump-115942015-12-21T02:39:58Z http://umpir.ump.edu.my/id/eprint/11594/ Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil Seydametova, Emine TP Chemical technology Hyperlipidemia is a main risk factor for coronary heart disease and stroke. The lipid-lowering drug, pravastatin, is currently obtained by the microbial biotransformation of compactin (mevastatin) to pravastatin. It is clear that pharmaceutical industry has to date utilized only a very minor portion of nature’s microbial arsenal for the discovery of pravastatin. Therefore, the search for microfungi which is able to produce this statin directly has gained increasing importance. In this study, a total of 33 soil fungal cultures were isolated from nine sampling sites in oil palm plantations in Malaysia and tested for their potential to produce pravastatin. The cultures were cultivated in submerged fermentation followed by screening for statin production using high-performance liquid chromatography. The best pravastatin producer was identified to species level using morphological characteristics and molecular biological approaches. From the tested cultures, 18 fungal isolates were able to produce pravastatin directly by fermentation. Among these, the isolate ESF20P was the best producer, with a yield of 15.8 mg/l pravastatin. Molecular identification of this strain showed the highest homology (98%) with Penicillium janthinellum. International Academy Publishing (IAP) 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/11594/1/Novel%20Pravastatin-Producing%20Penicillium%20janthinellum%20Strain%20Isolated%20from%20Soil.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/11594/7/Novel%20Pravastatin-Producing%20Penicillium%20janthinellum%20Strain%20Isolated%20from%20Soil.pdf Seydametova, Emine (2015) Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil. International Journal of Bioscience, Biochemistry and Bioinformatics, 5 (2). pp. 80-90. ISSN 2010-3638 http://www.ijbbb.org/vol5/397-B038.pdf doi: 10.17706/ijbbb.2015.5.2.80-90
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Seydametova, Emine Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil
description	Hyperlipidemia is a main risk factor for coronary heart disease and stroke. The lipid-lowering drug, pravastatin, is currently obtained by the microbial biotransformation of compactin (mevastatin) to pravastatin. It is clear that pharmaceutical industry has to date utilized only a very minor portion of nature’s microbial arsenal for the discovery of pravastatin. Therefore, the search for microfungi which is able to produce this statin directly has gained increasing importance. In this study, a total of 33 soil fungal cultures were isolated from nine sampling sites in oil palm plantations in Malaysia and tested for their potential to produce pravastatin. The cultures were cultivated in submerged fermentation followed by screening for statin production using high-performance liquid chromatography. The best pravastatin producer was identified to species level using morphological characteristics and molecular biological approaches. From the tested cultures, 18 fungal isolates were able to produce pravastatin directly by fermentation. Among these, the isolate ESF20P was the best producer, with a yield of 15.8 mg/l pravastatin. Molecular identification of this strain showed the highest homology (98%) with Penicillium janthinellum.
format	Article
author	Seydametova, Emine
author_facet	Seydametova, Emine
author_sort	Seydametova, Emine
title	Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil
title_short	Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil
title_full	Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil
title_fullStr	Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil
title_full_unstemmed	Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil
title_sort	novel pravastatin-producing penicillium janthinellum strain isolated from soil
publisher	International Academy Publishing (IAP)
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/11594/ http://umpir.ump.edu.my/id/eprint/11594/ http://umpir.ump.edu.my/id/eprint/11594/ http://umpir.ump.edu.my/id/eprint/11594/1/Novel%20Pravastatin-Producing%20Penicillium%20janthinellum%20Strain%20Isolated%20from%20Soil.pdf http://umpir.ump.edu.my/id/eprint/11594/7/Novel%20Pravastatin-Producing%20Penicillium%20janthinellum%20Strain%20Isolated%20from%20Soil.pdf
first_indexed	2023-09-18T22:12:31Z
last_indexed	2023-09-18T22:12:31Z
_version_	1777415121712185344

Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil

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