Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography

Membrane chromatography has been used widely as an alternative to the conventional packed bed chromatography for protein separation. Membrane chromatography used an adsorptive membrane that carried specific chromatography functionality. In the current study, a membrane chromatography was prepared by...

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Main Authors:	Syed Mohd Saufi, Tuan Chik, Mazrul Nizam, Abu Seman, Nurul Izwanie, Rasli
Format:	Article
Language:	English
Published:	Asian J. Chem 2016
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/12010/ http://umpir.ump.edu.my/id/eprint/12010/ http://umpir.ump.edu.my/id/eprint/12010/ http://umpir.ump.edu.my/id/eprint/12010/1/Comparison%20of%20Chemical%20and%20UV%20Photo-Grafting%20Modification%20on%20Polyamide.pdf

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spelling	ump-120102018-02-07T07:31:07Z http://umpir.ump.edu.my/id/eprint/12010/ Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography Syed Mohd Saufi, Tuan Chik Mazrul Nizam, Abu Seman Nurul Izwanie, Rasli TP Chemical technology Membrane chromatography has been used widely as an alternative to the conventional packed bed chromatography for protein separation. Membrane chromatography used an adsorptive membrane that carried specific chromatography functionality. In the current study, a membrane chromatography was prepared by modification of commercial polyamide microfiltration membrane with acrylic acid monomer. Two modification methods were compared which are UV photo grafting and chemical grafting via redox reaction. Modification parameters studied were initiator concentration (1-50 mM), monomer concentration (0.2-5 M) and reaction time (5-60 min). The highest lysozyme binding capacity achieved was 0.175 mg of lysozyme/cm2 membrane for the membrane prepared via UV photo grafting using 10 mM of photo-initiator, 0.1 M of acrylic acid and 15 min of reaction time. Asian J. Chem 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/12010/1/Comparison%20of%20Chemical%20and%20UV%20Photo-Grafting%20Modification%20on%20Polyamide.pdf Syed Mohd Saufi, Tuan Chik and Mazrul Nizam, Abu Seman and Nurul Izwanie, Rasli (2016) Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography. Asian Journal of Chemistry , 28 (2). pp. 395-398. ISSN 0970-7077 http://dx.doi.org/10.14233/ajchem.2016.19377 DOI: 10.14233/ajchem.2013.19377
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Syed Mohd Saufi, Tuan Chik Mazrul Nizam, Abu Seman Nurul Izwanie, Rasli Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography
description	Membrane chromatography has been used widely as an alternative to the conventional packed bed chromatography for protein separation. Membrane chromatography used an adsorptive membrane that carried specific chromatography functionality. In the current study, a membrane chromatography was prepared by modification of commercial polyamide microfiltration membrane with acrylic acid monomer. Two modification methods were compared which are UV photo grafting and chemical grafting via redox reaction. Modification parameters studied were initiator concentration (1-50 mM), monomer concentration (0.2-5 M) and reaction time (5-60 min). The highest lysozyme binding capacity achieved was 0.175 mg of lysozyme/cm2 membrane for the membrane prepared via UV photo grafting using 10 mM of photo-initiator, 0.1 M of acrylic acid and 15 min of reaction time.
format	Article
author	Syed Mohd Saufi, Tuan Chik Mazrul Nizam, Abu Seman Nurul Izwanie, Rasli
author_facet	Syed Mohd Saufi, Tuan Chik Mazrul Nizam, Abu Seman Nurul Izwanie, Rasli
author_sort	Syed Mohd Saufi, Tuan Chik
title	Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography
title_short	Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography
title_full	Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography
title_fullStr	Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography
title_full_unstemmed	Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography
title_sort	comparison of chemical and uv photo-grafting modification on polyamide microfiltration membrane for the preparation of membrane chromatography
publisher	Asian J. Chem
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/12010/ http://umpir.ump.edu.my/id/eprint/12010/ http://umpir.ump.edu.my/id/eprint/12010/ http://umpir.ump.edu.my/id/eprint/12010/1/Comparison%20of%20Chemical%20and%20UV%20Photo-Grafting%20Modification%20on%20Polyamide.pdf
first_indexed	2023-09-18T22:13:10Z
last_indexed	2023-09-18T22:13:10Z
_version_	1777415162730381312

Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography

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