Synergism Effect Between Inoculum Size and Aggregate Size on Flavonoid Production In Centella Asiatica (L.) Urban (Pegaga) Cell Suspension Cultures

The present study aimed to investigate the effect of different culture conditions on biomass content and flavonoid production of the elite C. asiatica accession UPM03. When 0.1 g inoculum 25 mL-1 of cell was grown in Murashige & Skoog (MS) medium supplemented with 2 mg 2,4-D l-1 and 1 mg kinetin...

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Main Authors:	Tan, Suat Hian, Maziah, Mahmood, Ariff, Arbakariya
Format:	Article
Language:	English
Published:	eSAT Publishing House Pvt. Ltd. 2013
Subjects:	SB Plant culture
Online Access:	http://umpir.ump.edu.my/id/eprint/12672/ http://umpir.ump.edu.my/id/eprint/12672/ http://umpir.ump.edu.my/id/eprint/12672/1/IJRET_110210037%202013.pdf

id	ump-12672
recordtype	eprints
spelling	ump-126722018-06-29T08:45:31Z http://umpir.ump.edu.my/id/eprint/12672/ Synergism Effect Between Inoculum Size and Aggregate Size on Flavonoid Production In Centella Asiatica (L.) Urban (Pegaga) Cell Suspension Cultures Tan, Suat Hian Maziah, Mahmood Ariff, Arbakariya SB Plant culture The present study aimed to investigate the effect of different culture conditions on biomass content and flavonoid production of the elite C. asiatica accession UPM03. When 0.1 g inoculum 25 mL-1 of cell was grown in Murashige & Skoog (MS) medium supplemented with 2 mg 2,4-D l-1 and 1 mg kinetin l-1, flavonoids were not significantly produced in cells or released into the culture medium. Production of flavonoid was correlated with the aggregation size and inoculum density. At aggregate size of 250-500 μm with 0.3 g inoculum density 25 mL-1, it gave the highest luteolin content with 35.45 μg g-1 DW. After investigating the effect of culture conditions, i.e. pH, inoculum density, light irradiation and plant growth regulator, we found that with the optimized condition (i.e.250-500 μm aggregate size, 0.5 g wet cell 25 mL-1 supplemented with 3 mg L-1 2,4-D and 1 mg L-1 kinetin at pH 5.7 under 16hphotoperiod) the luteolin content was 11-fold higher than the cell suspension cultured at an inoculum size of 0.3 g wet cell 25 mL-1 with 250-500 μm aggregate size. eSAT Publishing House Pvt. Ltd. 2013 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/12672/1/IJRET_110210037%202013.pdf Tan, Suat Hian and Maziah, Mahmood and Ariff, Arbakariya (2013) Synergism Effect Between Inoculum Size and Aggregate Size on Flavonoid Production In Centella Asiatica (L.) Urban (Pegaga) Cell Suspension Cultures. International Journal of Research in Engineering and Technology (IJRET), 2 (8). pp. 244-253. ISSN 2321-7308 (print); 2319-1163 (online) http://esatjournals.net/ijret/2013v02/i10/IJRET20130210037.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	SB Plant culture
spellingShingle	SB Plant culture Tan, Suat Hian Maziah, Mahmood Ariff, Arbakariya Synergism Effect Between Inoculum Size and Aggregate Size on Flavonoid Production In Centella Asiatica (L.) Urban (Pegaga) Cell Suspension Cultures
description	The present study aimed to investigate the effect of different culture conditions on biomass content and flavonoid production of the elite C. asiatica accession UPM03. When 0.1 g inoculum 25 mL-1 of cell was grown in Murashige & Skoog (MS) medium supplemented with 2 mg 2,4-D l-1 and 1 mg kinetin l-1, flavonoids were not significantly produced in cells or released into the culture medium. Production of flavonoid was correlated with the aggregation size and inoculum density. At aggregate size of 250-500 μm with 0.3 g inoculum density 25 mL-1, it gave the highest luteolin content with 35.45 μg g-1 DW. After investigating the effect of culture conditions, i.e. pH, inoculum density, light irradiation and plant growth regulator, we found that with the optimized condition (i.e.250-500 μm aggregate size, 0.5 g wet cell 25 mL-1 supplemented with 3 mg L-1 2,4-D and 1 mg L-1 kinetin at pH 5.7 under 16hphotoperiod) the luteolin content was 11-fold higher than the cell suspension cultured at an inoculum size of 0.3 g wet cell 25 mL-1 with 250-500 μm aggregate size.
format	Article
author	Tan, Suat Hian Maziah, Mahmood Ariff, Arbakariya
author_facet	Tan, Suat Hian Maziah, Mahmood Ariff, Arbakariya
author_sort	Tan, Suat Hian
title	Synergism Effect Between Inoculum Size and Aggregate Size on Flavonoid Production In Centella Asiatica (L.) Urban (Pegaga) Cell Suspension Cultures
title_short	Synergism Effect Between Inoculum Size and Aggregate Size on Flavonoid Production In Centella Asiatica (L.) Urban (Pegaga) Cell Suspension Cultures
title_full	Synergism Effect Between Inoculum Size and Aggregate Size on Flavonoid Production In Centella Asiatica (L.) Urban (Pegaga) Cell Suspension Cultures
title_fullStr	Synergism Effect Between Inoculum Size and Aggregate Size on Flavonoid Production In Centella Asiatica (L.) Urban (Pegaga) Cell Suspension Cultures
title_full_unstemmed	Synergism Effect Between Inoculum Size and Aggregate Size on Flavonoid Production In Centella Asiatica (L.) Urban (Pegaga) Cell Suspension Cultures
title_sort	synergism effect between inoculum size and aggregate size on flavonoid production in centella asiatica (l.) urban (pegaga) cell suspension cultures
publisher	eSAT Publishing House Pvt. Ltd.
publishDate	2013
url	http://umpir.ump.edu.my/id/eprint/12672/ http://umpir.ump.edu.my/id/eprint/12672/ http://umpir.ump.edu.my/id/eprint/12672/1/IJRET_110210037%202013.pdf
first_indexed	2023-09-18T22:14:31Z
last_indexed	2023-09-18T22:14:31Z
_version_	1777415248260628480

Synergism Effect Between Inoculum Size and Aggregate Size on Flavonoid Production In Centella Asiatica (L.) Urban (Pegaga) Cell Suspension Cultures

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