In-vitro biological activities of Au and Ag nanoparticles biosynthesized using commelina nudiflora L. aqueous extract

In this study, Commelina nudiflora L. aqueous extract was used as a reducing and stabilizing agent for the synthesis of metallic gold and silver nanoparticles. The physico-chemical and biological properties of the biosynthesized gold and silver nanoparticles were studied in a nanoscale regime. The s...

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Main Author:	K., Palaniselvam
Format:	Thesis
Language:	English English English
Published:	2015
Subjects:	QK Botany SB Plant culture
Online Access:	http://umpir.ump.edu.my/id/eprint/12956/ http://umpir.ump.edu.my/id/eprint/12956/ http://umpir.ump.edu.my/id/eprint/12956/1/FIST%20-%20K.%20PALANISELVAM%20-%20CD%209657.pdf http://umpir.ump.edu.my/id/eprint/12956/2/FIST%20-%20K.%20PALANISELVAM%20-%20CD%209657%20-%20CHAP%201.pdf http://umpir.ump.edu.my/id/eprint/12956/3/FIST%20-%20K.%20PALANISELVAM%20-%20CD%209657%20-%20CHAP%203.pdf

id	ump-12956
recordtype	eprints
spelling	ump-129562016-04-21T01:24:07Z http://umpir.ump.edu.my/id/eprint/12956/ In-vitro biological activities of Au and Ag nanoparticles biosynthesized using commelina nudiflora L. aqueous extract K., Palaniselvam QK Botany SB Plant culture In this study, Commelina nudiflora L. aqueous extract was used as a reducing and stabilizing agent for the synthesis of metallic gold and silver nanoparticles. The physico-chemical and biological properties of the biosynthesized gold and silver nanoparticles were studied in a nanoscale regime. The synthesized gold and silver nanoparticles physico-chemical properties were characterized by various analytical techniques such as UV-VIS, FESEM, XRD and FT-IR. The synthesized gold and silver nanoparticles were monodispersed, and the controlled shapes and tuneable surface properties were proven. Also, the reaction parameters such as pH, temperature, plant extract concentration and metal ion concentration have been optimized to synthesize the specific sizes and shapes of the nanoparticles. The synthesized gold and silver nanoparticles were spherical and triangular in shapes with the size range of between 25 to 45 nm and 50 to 150 nm respectively. The EDX spectra show strong peaks of both gold and silver nanoparticles which are more than 80% in the sample. The XRD data supports the claim that synthesized gold and silver nanoparticles are crystalline in nature. The plant extract contains various phytochemical constituents such as saponins, alkaloids, flavonoids and phenolic compounds. These secondary metabolites may be responsible for the Au and Ag ions reduction and also help in the formation of the metal nanoparticles. Furthermore, the in-vitro antioxidant ability of C. nudiflora extracts was studied by DPPH and ABTS radical scavenging assays. The aqueous plant extract showed significant activity in the free radical scavenging which were 63.4 mg/GAE and 49.10 mg/g in DPPH and ABTS respectively. Furthermore, the biosynthesized gold and silver nanoparticles have shown reduction in the cell viability and increased cytotoxicity on HCT-116 colon cancer cells with IC50 concentration of 200 and 100 μg/ml. The flow cytometry experiments revealed that the gold and silver nanoparticles treated cells increased DNA fragmentation and significant changes were observed in sub G1 cell cycle phases compared with positive control. Finally, the mRNA gene expressions of HCT-116 cells were studied by RT-qPCR techniques. The pro-apoptotic genes were highly expressed in the gold nanoparticles treated HCT-116 colon cancer model. The apopototic genes such as PUMA (++), caspase-3 (+) and caspase-8 (++) were moderately expressed in the treated samples compared with cisplatin. Overall, these findings prove that the C. nudiflora extract successfully synthesize metallic gold and silver nanoparticles with controlled size and shapes and also acts as a potent anti-colon cancer drug in the near future. 2015-09 Thesis NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/12956/1/FIST%20-%20K.%20PALANISELVAM%20-%20CD%209657.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/12956/2/FIST%20-%20K.%20PALANISELVAM%20-%20CD%209657%20-%20CHAP%201.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/12956/3/FIST%20-%20K.%20PALANISELVAM%20-%20CD%209657%20-%20CHAP%203.pdf K., Palaniselvam (2015) In-vitro biological activities of Au and Ag nanoparticles biosynthesized using commelina nudiflora L. aqueous extract. PhD thesis, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:94163&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English English
topic	QK Botany SB Plant culture
spellingShingle	QK Botany SB Plant culture K., Palaniselvam In-vitro biological activities of Au and Ag nanoparticles biosynthesized using commelina nudiflora L. aqueous extract
description	In this study, Commelina nudiflora L. aqueous extract was used as a reducing and stabilizing agent for the synthesis of metallic gold and silver nanoparticles. The physico-chemical and biological properties of the biosynthesized gold and silver nanoparticles were studied in a nanoscale regime. The synthesized gold and silver nanoparticles physico-chemical properties were characterized by various analytical techniques such as UV-VIS, FESEM, XRD and FT-IR. The synthesized gold and silver nanoparticles were monodispersed, and the controlled shapes and tuneable surface properties were proven. Also, the reaction parameters such as pH, temperature, plant extract concentration and metal ion concentration have been optimized to synthesize the specific sizes and shapes of the nanoparticles. The synthesized gold and silver nanoparticles were spherical and triangular in shapes with the size range of between 25 to 45 nm and 50 to 150 nm respectively. The EDX spectra show strong peaks of both gold and silver nanoparticles which are more than 80% in the sample. The XRD data supports the claim that synthesized gold and silver nanoparticles are crystalline in nature. The plant extract contains various phytochemical constituents such as saponins, alkaloids, flavonoids and phenolic compounds. These secondary metabolites may be responsible for the Au and Ag ions reduction and also help in the formation of the metal nanoparticles. Furthermore, the in-vitro antioxidant ability of C. nudiflora extracts was studied by DPPH and ABTS radical scavenging assays. The aqueous plant extract showed significant activity in the free radical scavenging which were 63.4 mg/GAE and 49.10 mg/g in DPPH and ABTS respectively. Furthermore, the biosynthesized gold and silver nanoparticles have shown reduction in the cell viability and increased cytotoxicity on HCT-116 colon cancer cells with IC50 concentration of 200 and 100 μg/ml. The flow cytometry experiments revealed that the gold and silver nanoparticles treated cells increased DNA fragmentation and significant changes were observed in sub G1 cell cycle phases compared with positive control. Finally, the mRNA gene expressions of HCT-116 cells were studied by RT-qPCR techniques. The pro-apoptotic genes were highly expressed in the gold nanoparticles treated HCT-116 colon cancer model. The apopototic genes such as PUMA (++), caspase-3 (+) and caspase-8 (++) were moderately expressed in the treated samples compared with cisplatin. Overall, these findings prove that the C. nudiflora extract successfully synthesize metallic gold and silver nanoparticles with controlled size and shapes and also acts as a potent anti-colon cancer drug in the near future.
format	Thesis
author	K., Palaniselvam
author_facet	K., Palaniselvam
author_sort	K., Palaniselvam
title	In-vitro biological activities of Au and Ag nanoparticles biosynthesized using commelina nudiflora L. aqueous extract
title_short	In-vitro biological activities of Au and Ag nanoparticles biosynthesized using commelina nudiflora L. aqueous extract
title_full	In-vitro biological activities of Au and Ag nanoparticles biosynthesized using commelina nudiflora L. aqueous extract
title_fullStr	In-vitro biological activities of Au and Ag nanoparticles biosynthesized using commelina nudiflora L. aqueous extract
title_full_unstemmed	In-vitro biological activities of Au and Ag nanoparticles biosynthesized using commelina nudiflora L. aqueous extract
title_sort	in-vitro biological activities of au and ag nanoparticles biosynthesized using commelina nudiflora l. aqueous extract
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/12956/ http://umpir.ump.edu.my/id/eprint/12956/ http://umpir.ump.edu.my/id/eprint/12956/1/FIST%20-%20K.%20PALANISELVAM%20-%20CD%209657.pdf http://umpir.ump.edu.my/id/eprint/12956/2/FIST%20-%20K.%20PALANISELVAM%20-%20CD%209657%20-%20CHAP%201.pdf http://umpir.ump.edu.my/id/eprint/12956/3/FIST%20-%20K.%20PALANISELVAM%20-%20CD%209657%20-%20CHAP%203.pdf
first_indexed	2023-09-18T22:15:01Z
last_indexed	2023-09-18T22:15:01Z
_version_	1777415279850029056

In-vitro biological activities of Au and Ag nanoparticles biosynthesized using commelina nudiflora L. aqueous extract

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