Antioxidant Activities of Extracts from Plants in Minor Uses: Parkia Speciosa, Vitex negundo, and Etlingera elatior

Our country is blessed with a huge number of plant species in our rainforest. According to World Wide Fund for Nature (WWF), the major types of forest in Malaysia are lowland dipterocarp forest, hill dipterocarp forest, upper hill dipterocarp forest and mangrove forest. Among of these abundance of p...

Full description

Bibliographic Details
Main Author:	Norasyidah, Harun
Format:	Thesis
Language:	English
Published:	2014
Subjects:	Q Science (General) S Agriculture (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/12982/ http://umpir.ump.edu.my/id/eprint/12982/ http://umpir.ump.edu.my/id/eprint/12982/1/FIST%20-%20NORASYIDAH%20BINTI%20HARUN%20-%20CD%209626.pdf

id	ump-12982
recordtype	eprints
spelling	ump-129822016-04-25T08:07:14Z http://umpir.ump.edu.my/id/eprint/12982/ Antioxidant Activities of Extracts from Plants in Minor Uses: Parkia Speciosa, Vitex negundo, and Etlingera elatior Norasyidah, Harun Q Science (General) S Agriculture (General) Our country is blessed with a huge number of plant species in our rainforest. According to World Wide Fund for Nature (WWF), the major types of forest in Malaysia are lowland dipterocarp forest, hill dipterocarp forest, upper hill dipterocarp forest and mangrove forest. Among of these abundance of plant sources, there are still a wide number of plants which can be classified as minor use, as they receive little research and therefore information on benefits of the plants are still limited. This current study is to evaluate the antioxidant activity of crude extracts of Parkia speciosa Hassk, Vitex negundo L., and Etlingera elatior Jack. The extraction were using reflux and maceration techniques in two different solubility which are 80% ethanol (aqueous ethanol) and total aqueous. Antioxidant was evaluated initially by thin layer chromatography with 2,2-Diphenyl-1-Picrylhydrazyl (TLC-DPPH) for screening, and quantitatively by DPPH radical scavenging assay and beta carotene bleaching assay with total phenolic and total flavonoid for additional information. Gas chromatography-mass spectrometry (GC-MS) was used for analyzing the volatile compounds existed in all extracts. TLC plate sprayed by DPPH solutions showed a positive reaction, where the yellowish white spot appeared on the purple background. P. speciosa (PSER) extracted in aqueous ethanol by reflux technique shows the best activity for both assays, with EC50 value for DPPH radical scavenging assay of 184.860 μg/mL and 57.595 % for antioxidant activity obtained from beta carotene bleaching assay. PSER also gave the highest value in total phenolic, which is 66.520 μg (GAE)/mg but low in total flavonoids content (TFC), which only 16.975 μg/mL as compared to the highest value showed by ethanolic V. negundo by reflux (VTER) with the value 50.060 μg/mL. These finding suggest that P. speciosa is a potential source of antioxidant that may be increasingly important for human consumption as well as assisting the antioxidant based industries. 2014-09 Thesis NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/12982/1/FIST%20-%20NORASYIDAH%20BINTI%20HARUN%20-%20CD%209626.pdf Norasyidah, Harun (2014) Antioxidant Activities of Extracts from Plants in Minor Uses: Parkia Speciosa, Vitex negundo, and Etlingera elatior. Masters thesis, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:94201&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) S Agriculture (General)
spellingShingle	Q Science (General) S Agriculture (General) Norasyidah, Harun Antioxidant Activities of Extracts from Plants in Minor Uses: Parkia Speciosa, Vitex negundo, and Etlingera elatior
description	Our country is blessed with a huge number of plant species in our rainforest. According to World Wide Fund for Nature (WWF), the major types of forest in Malaysia are lowland dipterocarp forest, hill dipterocarp forest, upper hill dipterocarp forest and mangrove forest. Among of these abundance of plant sources, there are still a wide number of plants which can be classified as minor use, as they receive little research and therefore information on benefits of the plants are still limited. This current study is to evaluate the antioxidant activity of crude extracts of Parkia speciosa Hassk, Vitex negundo L., and Etlingera elatior Jack. The extraction were using reflux and maceration techniques in two different solubility which are 80% ethanol (aqueous ethanol) and total aqueous. Antioxidant was evaluated initially by thin layer chromatography with 2,2-Diphenyl-1-Picrylhydrazyl (TLC-DPPH) for screening, and quantitatively by DPPH radical scavenging assay and beta carotene bleaching assay with total phenolic and total flavonoid for additional information. Gas chromatography-mass spectrometry (GC-MS) was used for analyzing the volatile compounds existed in all extracts. TLC plate sprayed by DPPH solutions showed a positive reaction, where the yellowish white spot appeared on the purple background. P. speciosa (PSER) extracted in aqueous ethanol by reflux technique shows the best activity for both assays, with EC50 value for DPPH radical scavenging assay of 184.860 μg/mL and 57.595 % for antioxidant activity obtained from beta carotene bleaching assay. PSER also gave the highest value in total phenolic, which is 66.520 μg (GAE)/mg but low in total flavonoids content (TFC), which only 16.975 μg/mL as compared to the highest value showed by ethanolic V. negundo by reflux (VTER) with the value 50.060 μg/mL. These finding suggest that P. speciosa is a potential source of antioxidant that may be increasingly important for human consumption as well as assisting the antioxidant based industries.
format	Thesis
author	Norasyidah, Harun
author_facet	Norasyidah, Harun
author_sort	Norasyidah, Harun
title	Antioxidant Activities of Extracts from Plants in Minor Uses: Parkia Speciosa, Vitex negundo, and Etlingera elatior
title_short	Antioxidant Activities of Extracts from Plants in Minor Uses: Parkia Speciosa, Vitex negundo, and Etlingera elatior
title_full	Antioxidant Activities of Extracts from Plants in Minor Uses: Parkia Speciosa, Vitex negundo, and Etlingera elatior
title_fullStr	Antioxidant Activities of Extracts from Plants in Minor Uses: Parkia Speciosa, Vitex negundo, and Etlingera elatior
title_full_unstemmed	Antioxidant Activities of Extracts from Plants in Minor Uses: Parkia Speciosa, Vitex negundo, and Etlingera elatior
title_sort	antioxidant activities of extracts from plants in minor uses: parkia speciosa, vitex negundo, and etlingera elatior
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/12982/ http://umpir.ump.edu.my/id/eprint/12982/ http://umpir.ump.edu.my/id/eprint/12982/1/FIST%20-%20NORASYIDAH%20BINTI%20HARUN%20-%20CD%209626.pdf
first_indexed	2023-09-18T22:15:05Z
last_indexed	2023-09-18T22:15:05Z
_version_	1777415283602882560

Antioxidant Activities of Extracts from Plants in Minor Uses: Parkia Speciosa, Vitex negundo, and Etlingera elatior

Similar Items