In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves

The present study aimed to study the anti-oxidant potential of isolated flavonoids from the ethanol extract (70%v/v) of Cassia tora leaves. Three flavonoids were isolated viz Luteolin-7-O-β-glucopyranoside (I), quercetin-3-O-β-d-glucuronide (II) and Formononetin-7-O-β-D-Glucoside (III) from the etha...

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Main Authors: Vijayalakshmi, A., Jayakumari, S., Masilamani, K.
Format: Article
Language:English
English
Published: Penerbit Universiti Malaysia Pahang 2016
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Online Access:http://umpir.ump.edu.my/id/eprint/13806/
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http://umpir.ump.edu.my/id/eprint/13806/
http://umpir.ump.edu.my/id/eprint/13806/1/In-vitro%20antioxidant%20potential%20of%20the%20flavonoid%20glycosides%20from%20Cassia%20tora%20linn.%20Leaves.pdf
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spelling ump-138062018-02-01T06:47:13Z http://umpir.ump.edu.my/id/eprint/13806/ In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves Vijayalakshmi, A. Jayakumari, S. Masilamani, K. T Technology (General) The present study aimed to study the anti-oxidant potential of isolated flavonoids from the ethanol extract (70%v/v) of Cassia tora leaves. Three flavonoids were isolated viz Luteolin-7-O-β-glucopyranoside (I), quercetin-3-O-β-d-glucuronide (II) and Formononetin-7-O-β-D-Glucoside (III) from the ethanol extract (70%v/v) of Cassia tora leaves in the previous study. A comprehensive study on the phytochemical contents - total phenolic content and flavonoid content was accessed using Folin-Denis and AlCl3 method respectively. The antioxidant potential of the samples was evaluated using inhibition of hydroxyl radical, 2,2-diphenyl-1- picrylhydrazyl (DPPH), and nitric oxide scavenging methods. The total phenolic content and flavonoid content was found to be 18.60 % w/w and 9.5% w/w respectively. The IC50 values of ethanol extract against hydroxyl, DPPH and nitric oxide radical were found to be 270 µg/mL, 190 µg/mL and 130 µg/mL respectively. The radical scavenging activity of the isolated flavonoids decreased in the following order: quercetin (IC50 values 15, 14, 18 µg/mL) >formononetin (IC50 values 19, 21, 14 µg/mL) > luteolin (IC50 values 20, 23, 18 µg/mL) respectively. Index terms - Cassia tora, flavonoids, HPTLC, antioxidant Penerbit Universiti Malaysia Pahang 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/13806/1/In-vitro%20antioxidant%20potential%20of%20the%20flavonoid%20glycosides%20from%20Cassia%20tora%20linn.%20Leaves.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/13806/7/ftech-2016-masilamani-Invitro%20antioxidant%20potential1.pdf Vijayalakshmi, A. and Jayakumari, S. and Masilamani, K. (2016) In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves. International Journal of Engineering Technology And Sciences (IJETS), 5 (1). pp. 19-26. ISSN 2289-697X http://ijets.ump.edu.my/images/archive/Vol5/IJETS3.pdf DOI: 10.15282/ijets.5.2016.1.3.1042
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
English
topic T Technology (General)
spellingShingle T Technology (General)
Vijayalakshmi, A.
Jayakumari, S.
Masilamani, K.
In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves
description The present study aimed to study the anti-oxidant potential of isolated flavonoids from the ethanol extract (70%v/v) of Cassia tora leaves. Three flavonoids were isolated viz Luteolin-7-O-β-glucopyranoside (I), quercetin-3-O-β-d-glucuronide (II) and Formononetin-7-O-β-D-Glucoside (III) from the ethanol extract (70%v/v) of Cassia tora leaves in the previous study. A comprehensive study on the phytochemical contents - total phenolic content and flavonoid content was accessed using Folin-Denis and AlCl3 method respectively. The antioxidant potential of the samples was evaluated using inhibition of hydroxyl radical, 2,2-diphenyl-1- picrylhydrazyl (DPPH), and nitric oxide scavenging methods. The total phenolic content and flavonoid content was found to be 18.60 % w/w and 9.5% w/w respectively. The IC50 values of ethanol extract against hydroxyl, DPPH and nitric oxide radical were found to be 270 µg/mL, 190 µg/mL and 130 µg/mL respectively. The radical scavenging activity of the isolated flavonoids decreased in the following order: quercetin (IC50 values 15, 14, 18 µg/mL) >formononetin (IC50 values 19, 21, 14 µg/mL) > luteolin (IC50 values 20, 23, 18 µg/mL) respectively. Index terms - Cassia tora, flavonoids, HPTLC, antioxidant
format Article
author Vijayalakshmi, A.
Jayakumari, S.
Masilamani, K.
author_facet Vijayalakshmi, A.
Jayakumari, S.
Masilamani, K.
author_sort Vijayalakshmi, A.
title In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves
title_short In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves
title_full In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves
title_fullStr In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves
title_full_unstemmed In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves
title_sort in-vitro antioxidant potential of the flavonoid glycosides from cassia tora linn. leaves
publisher Penerbit Universiti Malaysia Pahang
publishDate 2016
url http://umpir.ump.edu.my/id/eprint/13806/
http://umpir.ump.edu.my/id/eprint/13806/
http://umpir.ump.edu.my/id/eprint/13806/
http://umpir.ump.edu.my/id/eprint/13806/1/In-vitro%20antioxidant%20potential%20of%20the%20flavonoid%20glycosides%20from%20Cassia%20tora%20linn.%20Leaves.pdf
http://umpir.ump.edu.my/id/eprint/13806/7/ftech-2016-masilamani-Invitro%20antioxidant%20potential1.pdf
first_indexed 2023-09-18T22:16:50Z
last_indexed 2023-09-18T22:16:50Z
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