Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)

Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into t...

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Main Authors:	Wan Siti Atikah, Wan Omar, Ahmad Ziad, Sulaiman, Azilah, Ajit, Yusuf, Chisti, Mohd Hairul, Ab Rahim, Adam Leow, Thean Chor
Format:	Conference or Workshop Item
Language:	English
Published:	Universiti Malaysia Pahang 2016
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/15042/ http://umpir.ump.edu.my/id/eprint/15042/ http://umpir.ump.edu.my/id/eprint/15042/1/P011%20pg71-76.pdf

id	ump-15042
recordtype	eprints
spelling	ump-150422017-08-16T02:25:06Z http://umpir.ump.edu.my/id/eprint/15042/ Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) Wan Siti Atikah, Wan Omar Ahmad Ziad, Sulaiman Azilah, Ajit Yusuf, Chisti Mohd Hairul, Ab Rahim Adam Leow, Thean Chor TP Chemical technology Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into the same strain of competent cells would assist in research assessment. This study focused on the transformation of recombinant lipase from two different sources into Escherichia coli competent cells, BL21 (DE3). The recombinant cells, pUC8.2-14 (American Type Culture Collection (ATCC)®68046™) was harbouring Rhizopus (delemar) oryzae lipase and E. coli Top 10 pTrcHis was harbouring Staphylococcus hyicus lipase, respectively. The transformation is involved preparation of competent cells, polymerase chain reaction (PCR) and DNA sequencing. The DNA sequences obtained was inserted into Basic Local Alignment Search Tool (BLAST), under the databases of National Centre for Biotechnology Information (NCBI), USA. The findings show the respective plasmids were successfully carried by the E. coli BL21 (DE3) for further investigation. Universiti Malaysia Pahang 2016 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/15042/1/P011%20pg71-76.pdf Wan Siti Atikah, Wan Omar and Ahmad Ziad, Sulaiman and Azilah, Ajit and Yusuf, Chisti and Mohd Hairul, Ab Rahim and Adam Leow, Thean Chor (2016) Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3). In: Proceedings of The National Conference for Postgraduate Research (NCON-PGR 2016), 24-25 September 2016 , Universiti Malaysia Pahang (UMP), Pekan, Pahang. pp. 71-76.. http://ee.ump.edu.my/ncon/wp-content/uploads/2016/10/Proceeding-NCON-PGR-2016.zip
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Wan Siti Atikah, Wan Omar Ahmad Ziad, Sulaiman Azilah, Ajit Yusuf, Chisti Mohd Hairul, Ab Rahim Adam Leow, Thean Chor Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
description	Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into the same strain of competent cells would assist in research assessment. This study focused on the transformation of recombinant lipase from two different sources into Escherichia coli competent cells, BL21 (DE3). The recombinant cells, pUC8.2-14 (American Type Culture Collection (ATCC)®68046™) was harbouring Rhizopus (delemar) oryzae lipase and E. coli Top 10 pTrcHis was harbouring Staphylococcus hyicus lipase, respectively. The transformation is involved preparation of competent cells, polymerase chain reaction (PCR) and DNA sequencing. The DNA sequences obtained was inserted into Basic Local Alignment Search Tool (BLAST), under the databases of National Centre for Biotechnology Information (NCBI), USA. The findings show the respective plasmids were successfully carried by the E. coli BL21 (DE3) for further investigation.
format	Conference or Workshop Item
author	Wan Siti Atikah, Wan Omar Ahmad Ziad, Sulaiman Azilah, Ajit Yusuf, Chisti Mohd Hairul, Ab Rahim Adam Leow, Thean Chor
author_facet	Wan Siti Atikah, Wan Omar Ahmad Ziad, Sulaiman Azilah, Ajit Yusuf, Chisti Mohd Hairul, Ab Rahim Adam Leow, Thean Chor
author_sort	Wan Siti Atikah, Wan Omar
title	Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
title_short	Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
title_full	Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
title_fullStr	Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
title_full_unstemmed	Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
title_sort	transformation of ptrchis and puc8.2-14 into escherichia coli bl21 (de3)
publisher	Universiti Malaysia Pahang
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/15042/ http://umpir.ump.edu.my/id/eprint/15042/ http://umpir.ump.edu.my/id/eprint/15042/1/P011%20pg71-76.pdf
first_indexed	2023-09-18T22:19:19Z
last_indexed	2023-09-18T22:19:19Z
_version_	1777415549453598720

Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)

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