Extraction and Characterization of Keratin from Chicken Feather Waste Biomass: A study

Keratins are proteins that form hard fibers, present in wool, horns and hoofs, feathers etc. The feathers consist of up to 10% of total chicken weight. Uncontrolled disposal of feathers from the poultry slaughterhouses industry is troublesome to environment. Primary purification phase is necessary f...

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Main Authors:	Sharma, Swati, Gupta, Arun, Syed Mohd Saufi, Tuan Chik, Chua, Gek Kee, Pradeep Kumar , Podde, Jayshree , Thraisingam, Malini, Subramaniam
Format:	Conference or Workshop Item
Language:	English
Published:	Universiti Malaysia Pahang 2016
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/15774/ http://umpir.ump.edu.my/id/eprint/15774/ http://umpir.ump.edu.my/id/eprint/15774/1/P093%20pg693-699.pdf

id	ump-15774
recordtype	eprints
spelling	ump-157742019-09-10T03:33:41Z http://umpir.ump.edu.my/id/eprint/15774/ Extraction and Characterization of Keratin from Chicken Feather Waste Biomass: A study Sharma, Swati Gupta, Arun Syed Mohd Saufi, Tuan Chik Chua, Gek Kee Pradeep Kumar , Podde Jayshree , Thraisingam Malini, Subramaniam TP Chemical technology Keratins are proteins that form hard fibers, present in wool, horns and hoofs, feathers etc. The feathers consist of up to 10% of total chicken weight. Uncontrolled disposal of feathers from the poultry slaughterhouses industry is troublesome to environment. Primary purification phase is necessary for isolating the keratin from other materials. The present work was conducted to isolate the chicken feathers and to extract the keratin from processed biomass. The purification was conducted by washing with ddH2O, detergents and ether and lastly again with boiled water at (100oC). Reducing agents used were sodium sulfide and sodium sulfite. The precipitates obtained in the form of pellet were washed 2-3 times with ddH2O in order to remove foul smell and residues of sodium sulfide and HCl can be washed properly. To prepare the soluble form sodium hydroxide was added. Among the reducing agents tested Sodium sulfide showed good dissolving power as compared to others. Sodium sulfide with 0.3M and 0.5M concentrations dissolved the chicken feather efficiently. Keratin protein was dialyzed by ddH2O using cellulose membrane (12 MWCO) which retained the protein size ~12 kDa. The concentration of keratin was studied by Bradford method (1.6mg/ml) and physiochemical characterization was done by FTIR and TGA analysis. The presence of alkane, carboxylic acid, amine, thiocarbonyl groups and disulfide bond was confirmed by FTIR analysis. The purified keratin is a promising substrate for the synthesis of cosmetic and pharmaceutical products. Universiti Malaysia Pahang 2016 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/15774/1/P093%20pg693-699.pdf Sharma, Swati and Gupta, Arun and Syed Mohd Saufi, Tuan Chik and Chua, Gek Kee and Pradeep Kumar , Podde and Jayshree , Thraisingam and Malini, Subramaniam (2016) Extraction and Characterization of Keratin from Chicken Feather Waste Biomass: A study. In: Proceedings of The National Conference for Postgraduate Research (NCON-PGR 2016), 24-25 September 2016 , Universiti Malaysia Pahang (UMP), Pekan, Pahang. pp. 693-699.. http://ee.ump.edu.my/ncon/wp-content/uploads/2016/10/Proceeding-NCON-PGR-2016.zip
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Sharma, Swati Gupta, Arun Syed Mohd Saufi, Tuan Chik Chua, Gek Kee Pradeep Kumar , Podde Jayshree , Thraisingam Malini, Subramaniam Extraction and Characterization of Keratin from Chicken Feather Waste Biomass: A study
description	Keratins are proteins that form hard fibers, present in wool, horns and hoofs, feathers etc. The feathers consist of up to 10% of total chicken weight. Uncontrolled disposal of feathers from the poultry slaughterhouses industry is troublesome to environment. Primary purification phase is necessary for isolating the keratin from other materials. The present work was conducted to isolate the chicken feathers and to extract the keratin from processed biomass. The purification was conducted by washing with ddH2O, detergents and ether and lastly again with boiled water at (100oC). Reducing agents used were sodium sulfide and sodium sulfite. The precipitates obtained in the form of pellet were washed 2-3 times with ddH2O in order to remove foul smell and residues of sodium sulfide and HCl can be washed properly. To prepare the soluble form sodium hydroxide was added. Among the reducing agents tested Sodium sulfide showed good dissolving power as compared to others. Sodium sulfide with 0.3M and 0.5M concentrations dissolved the chicken feather efficiently. Keratin protein was dialyzed by ddH2O using cellulose membrane (12 MWCO) which retained the protein size ~12 kDa. The concentration of keratin was studied by Bradford method (1.6mg/ml) and physiochemical characterization was done by FTIR and TGA analysis. The presence of alkane, carboxylic acid, amine, thiocarbonyl groups and disulfide bond was confirmed by FTIR analysis. The purified keratin is a promising substrate for the synthesis of cosmetic and pharmaceutical products.
format	Conference or Workshop Item
author	Sharma, Swati Gupta, Arun Syed Mohd Saufi, Tuan Chik Chua, Gek Kee Pradeep Kumar , Podde Jayshree , Thraisingam Malini, Subramaniam
author_facet	Sharma, Swati Gupta, Arun Syed Mohd Saufi, Tuan Chik Chua, Gek Kee Pradeep Kumar , Podde Jayshree , Thraisingam Malini, Subramaniam
author_sort	Sharma, Swati
title	Extraction and Characterization of Keratin from Chicken Feather Waste Biomass: A study
title_short	Extraction and Characterization of Keratin from Chicken Feather Waste Biomass: A study
title_full	Extraction and Characterization of Keratin from Chicken Feather Waste Biomass: A study
title_fullStr	Extraction and Characterization of Keratin from Chicken Feather Waste Biomass: A study
title_full_unstemmed	Extraction and Characterization of Keratin from Chicken Feather Waste Biomass: A study
title_sort	extraction and characterization of keratin from chicken feather waste biomass: a study
publisher	Universiti Malaysia Pahang
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/15774/ http://umpir.ump.edu.my/id/eprint/15774/ http://umpir.ump.edu.my/id/eprint/15774/1/P093%20pg693-699.pdf
first_indexed	2023-09-18T22:20:48Z
last_indexed	2023-09-18T22:20:48Z
_version_	1777415643377696768

Extraction and Characterization of Keratin from Chicken Feather Waste Biomass: A study

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