Synthesis and Cytotoxic Effects of (E)-3-(2,3-dimethoxyphenyl)-1-(5-methylfuran-2-yl) prop-2-en-1-one in MDA-MB231 and MCF-7 Breast Cancer Cell Lines

Synthesis and Cytotoxic Effects of (E)-3-(2,3-dimethoxyphenyl)-1-(5-methylfuran-2-yl) prop-2-en-1-one in MDA-MB231 and MCF-7 Breast Cancer Cell Lines

A chalcone derivative, (E)-3-(2,3-dimethoxyphenyl)-1-(5-methylfuran-2-yl)-prop-2-en-1-one (DMMF) was synthesized and evaluated against various cancerous cell lines including colon adenocarcinoma (HT-29), myloplasticleukemia (HL60), breast cancer (MCF-7 and MDA-MB231), normal hepatic cell (WRL-68) an...

Full description

Bibliographic Details
Main Authors: Akhtar, Muhammad Nadeem, Ali Salim, Landa Zeenelabdin, Swee, Keong Yeap, Nadiah, Abu, Seema, Zareen, Kong, Mun Lo, Addila, Abu Bakar, Noorjahan Banu, Alitheen
Format: Article
Language:English
Published: Elsevier Ltd 2017
Subjects:
Q Science (General)
Online Access:http://umpir.ump.edu.my/id/eprint/16454/
http://umpir.ump.edu.my/id/eprint/16454/
http://umpir.ump.edu.my/id/eprint/16454/
http://umpir.ump.edu.my/id/eprint/16454/1/fist-2017-nadeem-Synthesis%20and%20cytotoxic1.pdf
id ump-16454
recordtype eprints
spelling ump-164542019-10-30T04:14:10Z http://umpir.ump.edu.my/id/eprint/16454/ Synthesis and Cytotoxic Effects of (E)-3-(2,3-dimethoxyphenyl)-1-(5-methylfuran-2-yl) prop-2-en-1-one in MDA-MB231 and MCF-7 Breast Cancer Cell Lines Akhtar, Muhammad Nadeem Ali Salim, Landa Zeenelabdin Swee, Keong Yeap Nadiah, Abu Seema, Zareen Kong, Mun Lo Addila, Abu Bakar Noorjahan Banu, Alitheen Q Science (General) A chalcone derivative, (E)-3-(2,3-dimethoxyphenyl)-1-(5-methylfuran-2-yl)-prop-2-en-1-one (DMMF) was synthesized and evaluated against various cancerous cell lines including colon adenocarcinoma (HT-29), myloplasticleukemia (HL60), breast cancer (MCF-7 and MDA-MB231), normal hepatic cell (WRL-68) and normal breast cell (MCF-10A). The structure of DMMF was determined by EI-MS, 1H NMR and single X-ray crystallographic techniques. The DMMF possessed the highest cytotoxic effect against MCF-7 breast cancer cell (2.01 ± 1.53 μg/mL) and lowest against normal hepatic WRL-68 and breast cell lines after 24 h of treatment. Induction of apoptosis and regulation of cell cycle progression results indicates the significant increase in early apoptosis and G2/M arrest after 48 h of treatment in MCF-7 cells. Meanwhile, in MDA-MB231 cells, there was an increase in Sub G0/G1 cells population and early/late apoptotic cells upon treatment with DMMF. Additionally, DMMF effectively induced G2/M cell cycle arrest in MCF-7 cells and apoptosis in both MCF-7 and MDA-MB231 cells. Elsevier Ltd 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/16454/1/fist-2017-nadeem-Synthesis%20and%20cytotoxic1.pdf Akhtar, Muhammad Nadeem and Ali Salim, Landa Zeenelabdin and Swee, Keong Yeap and Nadiah, Abu and Seema, Zareen and Kong, Mun Lo and Addila, Abu Bakar and Noorjahan Banu, Alitheen (2017) Synthesis and Cytotoxic Effects of (E)-3-(2,3-dimethoxyphenyl)-1-(5-methylfuran-2-yl) prop-2-en-1-one in MDA-MB231 and MCF-7 Breast Cancer Cell Lines. Phytochemistry Letters, 19. pp. 145-150. ISSN 1874-3900 https://doi.org/10.1016/j.phytol.2016.12.022 DOI: 10.1016/j.phytol.2016.12.022
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic Q Science (General)
spellingShingle Q Science (General)
Akhtar, Muhammad Nadeem
Ali Salim, Landa Zeenelabdin
Swee, Keong Yeap
Nadiah, Abu
Seema, Zareen
Kong, Mun Lo
Addila, Abu Bakar
Noorjahan Banu, Alitheen
Synthesis and Cytotoxic Effects of (E)-3-(2,3-dimethoxyphenyl)-1-(5-methylfuran-2-yl) prop-2-en-1-one in MDA-MB231 and MCF-7 Breast Cancer Cell Lines
description A chalcone derivative, (E)-3-(2,3-dimethoxyphenyl)-1-(5-methylfuran-2-yl)-prop-2-en-1-one (DMMF) was synthesized and evaluated against various cancerous cell lines including colon adenocarcinoma (HT-29), myloplasticleukemia (HL60), breast cancer (MCF-7 and MDA-MB231), normal hepatic cell (WRL-68) and normal breast cell (MCF-10A). The structure of DMMF was determined by EI-MS, 1H NMR and single X-ray crystallographic techniques. The DMMF possessed the highest cytotoxic effect against MCF-7 breast cancer cell (2.01 ± 1.53 μg/mL) and lowest against normal hepatic WRL-68 and breast cell lines after 24 h of treatment. Induction of apoptosis and regulation of cell cycle progression results indicates the significant increase in early apoptosis and G2/M arrest after 48 h of treatment in MCF-7 cells. Meanwhile, in MDA-MB231 cells, there was an increase in Sub G0/G1 cells population and early/late apoptotic cells upon treatment with DMMF. Additionally, DMMF effectively induced G2/M cell cycle arrest in MCF-7 cells and apoptosis in both MCF-7 and MDA-MB231 cells.
format Article
author Akhtar, Muhammad Nadeem
Ali Salim, Landa Zeenelabdin
Swee, Keong Yeap
Nadiah, Abu
Seema, Zareen
Kong, Mun Lo
Addila, Abu Bakar
Noorjahan Banu, Alitheen
author_facet Akhtar, Muhammad Nadeem
Ali Salim, Landa Zeenelabdin
Swee, Keong Yeap
Nadiah, Abu
Seema, Zareen
Kong, Mun Lo
Addila, Abu Bakar
Noorjahan Banu, Alitheen
author_sort Akhtar, Muhammad Nadeem
title Synthesis and Cytotoxic Effects of (E)-3-(2,3-dimethoxyphenyl)-1-(5-methylfuran-2-yl) prop-2-en-1-one in MDA-MB231 and MCF-7 Breast Cancer Cell Lines
title_short Synthesis and Cytotoxic Effects of (E)-3-(2,3-dimethoxyphenyl)-1-(5-methylfuran-2-yl) prop-2-en-1-one in MDA-MB231 and MCF-7 Breast Cancer Cell Lines
title_full Synthesis and Cytotoxic Effects of (E)-3-(2,3-dimethoxyphenyl)-1-(5-methylfuran-2-yl) prop-2-en-1-one in MDA-MB231 and MCF-7 Breast Cancer Cell Lines
title_fullStr Synthesis and Cytotoxic Effects of (E)-3-(2,3-dimethoxyphenyl)-1-(5-methylfuran-2-yl) prop-2-en-1-one in MDA-MB231 and MCF-7 Breast Cancer Cell Lines
title_full_unstemmed Synthesis and Cytotoxic Effects of (E)-3-(2,3-dimethoxyphenyl)-1-(5-methylfuran-2-yl) prop-2-en-1-one in MDA-MB231 and MCF-7 Breast Cancer Cell Lines
title_sort synthesis and cytotoxic effects of (e)-3-(2,3-dimethoxyphenyl)-1-(5-methylfuran-2-yl) prop-2-en-1-one in mda-mb231 and mcf-7 breast cancer cell lines
publisher Elsevier Ltd
publishDate 2017
url http://umpir.ump.edu.my/id/eprint/16454/
http://umpir.ump.edu.my/id/eprint/16454/
http://umpir.ump.edu.my/id/eprint/16454/
http://umpir.ump.edu.my/id/eprint/16454/1/fist-2017-nadeem-Synthesis%20and%20cytotoxic1.pdf
first_indexed 2023-09-18T22:22:09Z
last_indexed 2023-09-18T22:22:09Z
_version_ 1777415727788064768

Similar Items