Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells

Flavokawain B (FKB) is known to possess promising anticancer abilities. This is demonstrated in various cancer cell lines including HeLa cells. Cervical cancer is among the most widely diagnosed cancer among women today. Though FKB has been shown to be effective in treating cancer cells, the exact m...

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Main Authors:	Swee, Keong Yeap, Nadiah, Abu, Akhtar, Muhammad Nadeem, Wan, Yong Ho, Huynh, Ky, Sheau, Wei Tan, Noorjahan Banu, Alitheen, Tunku, Kamarul
Format:	Article
Language:	English
Published:	SAGE Publications 2017
Subjects:	QD Chemistry
Online Access:	http://umpir.ump.edu.my/id/eprint/16811/ http://umpir.ump.edu.my/id/eprint/16811/ http://umpir.ump.edu.my/id/eprint/16811/ http://umpir.ump.edu.my/id/eprint/16811/1/fist-2016-nadeem-Gene%20Expression%20Analysis%20Reveals%20the3.pdf

id	ump-16811
recordtype	eprints
spelling	ump-168112017-09-13T06:12:55Z http://umpir.ump.edu.my/id/eprint/16811/ Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells Swee, Keong Yeap Nadiah, Abu Akhtar, Muhammad Nadeem Wan, Yong Ho Huynh, Ky Sheau, Wei Tan Noorjahan Banu, Alitheen Tunku, Kamarul QD Chemistry Flavokawain B (FKB) is known to possess promising anticancer abilities. This is demonstrated in various cancer cell lines including HeLa cells. Cervical cancer is among the most widely diagnosed cancer among women today. Though FKB has been shown to be effective in treating cancer cells, the exact molecular mechanism is still unknown. This study is aimed at understanding the effects of FKB on HeLa cells using a microarray-based mRNA expression profiling and proteome profiling of stress-related proteins. The results of this study suggest that FKB induced cell death through p21-mediated cell cycle arrest and activation of p38. However, concurrent activation of antioxidant-related pathways and iron sequestration pathway followed by activation of ER-resident stress proteins clearly indicate that FKB failed to induce apoptosis in HeLa cells via oxidative stress. This effect implies that the protection of HeLa cells by FKB from H2O2–induced cell death is via neutralization of reactive oxygen species. SAGE Publications 2017 Article PeerReviewed application/pdf en cc_by_nc http://umpir.ump.edu.my/id/eprint/16811/1/fist-2016-nadeem-Gene%20Expression%20Analysis%20Reveals%20the3.pdf Swee, Keong Yeap and Nadiah, Abu and Akhtar, Muhammad Nadeem and Wan, Yong Ho and Huynh, Ky and Sheau, Wei Tan and Noorjahan Banu, Alitheen and Tunku, Kamarul (2017) Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells. Integrative Cancer Therapies, 16 (3). pp. 373-384. ISSN 1534-7354 (print); 1552-695X (online) http://dx.doi.org/10.1177/1534735416660383 DOI: 10.1177/1534735416660383
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Swee, Keong Yeap Nadiah, Abu Akhtar, Muhammad Nadeem Wan, Yong Ho Huynh, Ky Sheau, Wei Tan Noorjahan Banu, Alitheen Tunku, Kamarul Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells
description	Flavokawain B (FKB) is known to possess promising anticancer abilities. This is demonstrated in various cancer cell lines including HeLa cells. Cervical cancer is among the most widely diagnosed cancer among women today. Though FKB has been shown to be effective in treating cancer cells, the exact molecular mechanism is still unknown. This study is aimed at understanding the effects of FKB on HeLa cells using a microarray-based mRNA expression profiling and proteome profiling of stress-related proteins. The results of this study suggest that FKB induced cell death through p21-mediated cell cycle arrest and activation of p38. However, concurrent activation of antioxidant-related pathways and iron sequestration pathway followed by activation of ER-resident stress proteins clearly indicate that FKB failed to induce apoptosis in HeLa cells via oxidative stress. This effect implies that the protection of HeLa cells by FKB from H2O2–induced cell death is via neutralization of reactive oxygen species.
format	Article
author	Swee, Keong Yeap Nadiah, Abu Akhtar, Muhammad Nadeem Wan, Yong Ho Huynh, Ky Sheau, Wei Tan Noorjahan Banu, Alitheen Tunku, Kamarul
author_facet	Swee, Keong Yeap Nadiah, Abu Akhtar, Muhammad Nadeem Wan, Yong Ho Huynh, Ky Sheau, Wei Tan Noorjahan Banu, Alitheen Tunku, Kamarul
author_sort	Swee, Keong Yeap
title	Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells
title_short	Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells
title_full	Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells
title_fullStr	Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells
title_full_unstemmed	Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells
title_sort	gene expression analysis reveals the concurrent activation of proapoptotic and antioxidant-defensive mechanisms in flavokawain b-treated cervical cancer hela cells
publisher	SAGE Publications
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/16811/ http://umpir.ump.edu.my/id/eprint/16811/ http://umpir.ump.edu.my/id/eprint/16811/ http://umpir.ump.edu.my/id/eprint/16811/1/fist-2016-nadeem-Gene%20Expression%20Analysis%20Reveals%20the3.pdf
first_indexed	2023-09-18T22:22:49Z
last_indexed	2023-09-18T22:22:49Z
_version_	1777415769907265536

Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells

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