Bioassay-guided Isolation and Identification of Antifungal Compounds from Seeds of Swietenia Macrophylla King

Swietenia macrophylla King, a plant used extensively in many countries for medicinal purposes. The objective of the present study was to investigate in vitro antifungal activity of the extract as well as bioassay-guided isolation and identification of compounds from most active fraction of...

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Main Authors:	Azhari, H. Nour, Mohammed, Sulieman, M. M., Yusoff, Nour, A. H., Mazza, M. Adam
Format:	Article
Language:	English
Published:	AENSI Publishing 2016
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/16840/ http://umpir.ump.edu.my/id/eprint/16840/ http://umpir.ump.edu.my/id/eprint/16840/1/fkksa-2016-nhabdurahman-Bioassay%20guided%20isolation.pdf

id	ump-16840
recordtype	eprints
spelling	ump-168402018-03-08T06:09:48Z http://umpir.ump.edu.my/id/eprint/16840/ Bioassay-guided Isolation and Identification of Antifungal Compounds from Seeds of Swietenia Macrophylla King Azhari, H. Nour Mohammed, Sulieman M. M., Yusoff Nour, A. H. Mazza, M. Adam TP Chemical technology Swietenia macrophylla King, a plant used extensively in many countries for medicinal purposes. The objective of the present study was to investigate in vitro antifungal activity of the extract as well as bioassay-guided isolation and identification of compounds from most active fraction of S. macrophylla seeds. In the antifungal assay, three clinical fungal strains; namely, Aspergillus flavus, Candida albicans and Aspergillus niger were tested by disc diffusion method. Solvent partitioning followed by Preparative High Performance Liquid Chromatography (prep-HPLC) of the most active fraction afforded four known limonoids and identified by spectroscopic techniques including Fourier Transform Infrared Spectroscopy (FTIR), Mass Spectrometry (MS) and Nuclear Magnetic Resonance Spectroscopy (NMR) analyses. The compounds were: swietenolide (1), proceranolide (2), 3-O-tigloyl-6-O-acetylswietenolide (3) and swietenine acetate (4). Among the fractions, ethyl acetate was the most dominant and effective fraction. Moreover, three compounds (1-3) showed moderate to weak activity, whereas all tested organisms completely resistant to compound 4 and no inhibition zone was observed. On overall, among the three active compounds; compound 1 showed more potent activity than other compounds against all tested organisms. AENSI Publishing 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/16840/1/fkksa-2016-nhabdurahman-Bioassay%20guided%20isolation.pdf Azhari, H. Nour and Mohammed, Sulieman and M. M., Yusoff and Nour, A. H. and Mazza, M. Adam (2016) Bioassay-guided Isolation and Identification of Antifungal Compounds from Seeds of Swietenia Macrophylla King. Australian Journal of Basic and Applied Sciences, 10 (17 Sp.). pp. 55-62. ISSN 1991-8178 http://ajbasweb.com/old/ajbas/2016/Special%20ICCEIB/55-62.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Azhari, H. Nour Mohammed, Sulieman M. M., Yusoff Nour, A. H. Mazza, M. Adam Bioassay-guided Isolation and Identification of Antifungal Compounds from Seeds of Swietenia Macrophylla King
description	Swietenia macrophylla King, a plant used extensively in many countries for medicinal purposes. The objective of the present study was to investigate in vitro antifungal activity of the extract as well as bioassay-guided isolation and identification of compounds from most active fraction of S. macrophylla seeds. In the antifungal assay, three clinical fungal strains; namely, Aspergillus flavus, Candida albicans and Aspergillus niger were tested by disc diffusion method. Solvent partitioning followed by Preparative High Performance Liquid Chromatography (prep-HPLC) of the most active fraction afforded four known limonoids and identified by spectroscopic techniques including Fourier Transform Infrared Spectroscopy (FTIR), Mass Spectrometry (MS) and Nuclear Magnetic Resonance Spectroscopy (NMR) analyses. The compounds were: swietenolide (1), proceranolide (2), 3-O-tigloyl-6-O-acetylswietenolide (3) and swietenine acetate (4). Among the fractions, ethyl acetate was the most dominant and effective fraction. Moreover, three compounds (1-3) showed moderate to weak activity, whereas all tested organisms completely resistant to compound 4 and no inhibition zone was observed. On overall, among the three active compounds; compound 1 showed more potent activity than other compounds against all tested organisms.
format	Article
author	Azhari, H. Nour Mohammed, Sulieman M. M., Yusoff Nour, A. H. Mazza, M. Adam
author_facet	Azhari, H. Nour Mohammed, Sulieman M. M., Yusoff Nour, A. H. Mazza, M. Adam
author_sort	Azhari, H. Nour
title	Bioassay-guided Isolation and Identification of Antifungal Compounds from Seeds of Swietenia Macrophylla King
title_short	Bioassay-guided Isolation and Identification of Antifungal Compounds from Seeds of Swietenia Macrophylla King
title_full	Bioassay-guided Isolation and Identification of Antifungal Compounds from Seeds of Swietenia Macrophylla King
title_fullStr	Bioassay-guided Isolation and Identification of Antifungal Compounds from Seeds of Swietenia Macrophylla King
title_full_unstemmed	Bioassay-guided Isolation and Identification of Antifungal Compounds from Seeds of Swietenia Macrophylla King
title_sort	bioassay-guided isolation and identification of antifungal compounds from seeds of swietenia macrophylla king
publisher	AENSI Publishing
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/16840/ http://umpir.ump.edu.my/id/eprint/16840/ http://umpir.ump.edu.my/id/eprint/16840/1/fkksa-2016-nhabdurahman-Bioassay%20guided%20isolation.pdf
first_indexed	2023-09-18T22:22:52Z
last_indexed	2023-09-18T22:22:52Z
_version_	1777415772955475968

Bioassay-guided Isolation and Identification of Antifungal Compounds from Seeds of Swietenia Macrophylla King

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