Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells
The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid b-peptide 1 –42 length (Ab1 – 42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250mM H2O2 or 20 mM Ab1 – 42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of c...
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Taylor & Francis
2015
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| Online Access: | http://umpir.ump.edu.my/id/eprint/16897/ http://umpir.ump.edu.my/id/eprint/16897/ http://umpir.ump.edu.my/id/eprint/16897/ http://umpir.ump.edu.my/id/eprint/16897/1/fist-2015-sharina-Neuroprotective%20effect1.pdf |
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ump-168972018-04-19T02:34:26Z http://umpir.ump.edu.my/id/eprint/16897/ Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells Norsharina, Ismail Akhtar, Muhammad Nadeem Maznah, Ismail Seema, Zareen Syed Adnan, Ali Shah Nordin, Hj Lajis Saiful Nizam, Tajuddin Q Science (General) The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid b-peptide 1 –42 length (Ab1 – 42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250mM H2O2 or 20 mM Ab1 – 42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells with ethyl acetate extract (EAE) or n-butanol extract (BE) at 300 mg/mL and then exposure to H2O2 protected the cells against the neurotoxic effects of H2O2. Besides, methanolic extract (ME) at 1 and 10 mg/mL exerted neuroprotective effect on Ab1 – 42-induced toxicity to the cells. These results showed that EAE, BE and ME exhibited neuroprotective activities against H2O2- and Ab1 – 42-induced cell death. Flavonoids (3–6) and b-sitosterol glucoside (8) were isolated from the EAE. Compound 1 was isolated from hexane extract, and compounds 2 and 7 were isolated from dichloromethane extract. All these observations provide the possible evidence for contribution in the neuroprotective effects. Taylor & Francis 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/16897/1/fist-2015-sharina-Neuroprotective%20effect1.pdf Norsharina, Ismail and Akhtar, Muhammad Nadeem and Maznah, Ismail and Seema, Zareen and Syed Adnan, Ali Shah and Nordin, Hj Lajis and Saiful Nizam, Tajuddin (2015) Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells. Natural Product Research: Formerly Natural Product Letters, 29 (16). pp. 1571-1574. ISSN 1478-6419 (print); 1478-6427 (online) http://www.tandfonline.com/doi/abs/10.1080/14786419.2014.985676 DOI: 10.1080/14786419.2014.985676 |
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Digital Repository |
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Local University |
| institution |
Universiti Malaysia Pahang |
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UMP Institutional Repository |
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Online Access |
| language |
English |
| topic |
Q Science (General) |
| spellingShingle |
Q Science (General) Norsharina, Ismail Akhtar, Muhammad Nadeem Maznah, Ismail Seema, Zareen Syed Adnan, Ali Shah Nordin, Hj Lajis Saiful Nizam, Tajuddin Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells |
| description |
The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid b-peptide 1 –42 length (Ab1 – 42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250mM H2O2 or 20 mM Ab1 – 42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells with ethyl acetate extract (EAE) or n-butanol extract (BE) at 300 mg/mL and then exposure to H2O2 protected the cells against the neurotoxic effects of H2O2. Besides, methanolic extract (ME) at 1 and 10 mg/mL exerted neuroprotective effect on Ab1 – 42-induced toxicity to the cells. These results showed that EAE, BE and ME exhibited neuroprotective activities against H2O2- and Ab1 – 42-induced cell death. Flavonoids (3–6) and b-sitosterol glucoside (8) were isolated from the EAE. Compound 1 was isolated from hexane extract, and compounds 2 and 7 were isolated from dichloromethane extract. All these observations provide the possible evidence for contribution in the neuroprotective effects. |
| format |
Article |
| author |
Norsharina, Ismail Akhtar, Muhammad Nadeem Maznah, Ismail Seema, Zareen Syed Adnan, Ali Shah Nordin, Hj Lajis Saiful Nizam, Tajuddin |
| author_facet |
Norsharina, Ismail Akhtar, Muhammad Nadeem Maznah, Ismail Seema, Zareen Syed Adnan, Ali Shah Nordin, Hj Lajis Saiful Nizam, Tajuddin |
| author_sort |
Norsharina, Ismail |
| title |
Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells |
| title_short |
Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells |
| title_full |
Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells |
| title_fullStr |
Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells |
| title_full_unstemmed |
Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells |
| title_sort |
neuroprotective effect from stem bark extracts of knema laurina against h2o2-and aβ1-42-induced cell death in human sh-sy5y cells |
| publisher |
Taylor & Francis |
| publishDate |
2015 |
| url |
http://umpir.ump.edu.my/id/eprint/16897/ http://umpir.ump.edu.my/id/eprint/16897/ http://umpir.ump.edu.my/id/eprint/16897/ http://umpir.ump.edu.my/id/eprint/16897/1/fist-2015-sharina-Neuroprotective%20effect1.pdf |
| first_indexed |
2023-09-18T22:22:58Z |
| last_indexed |
2023-09-18T22:22:58Z |
| _version_ |
1777415779104325632 |