Optimization Of Sorbitol Production Using Immobilized Of Lactobacillus Plantarum Strain (BAA-793) Via Solid State Fermentation (SSF) Process: Response Surface Methodology (RSM)

Background: The process introduced in this work is the production of sorbitol via solid state fermentation (SSF) using immobilized cells of bacteria. The raw material that was used in this study is Meranti wood sawdust (MWS) that was obtained from Kilang Kayu Aman, Gambang Kuantan, Pahang. The bacte...

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Main Authors:	Zuriana, Sidi Ahmad, Mimi Sakinah, A. M., Farhan, Mohd Said
Format:	Article
Language:	English
Published:	AENSI Publishing 2016
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/17288/ http://umpir.ump.edu.my/id/eprint/17288/ http://umpir.ump.edu.my/id/eprint/17288/1/ftech-2016-mimi-Optimization%20Of%20Sorbitol%20Production%20Using%20Immobilized.pdf

id	ump-17288
recordtype	eprints
spelling	ump-172882018-01-17T06:17:47Z http://umpir.ump.edu.my/id/eprint/17288/ Optimization Of Sorbitol Production Using Immobilized Of Lactobacillus Plantarum Strain (BAA-793) Via Solid State Fermentation (SSF) Process: Response Surface Methodology (RSM) Zuriana, Sidi Ahmad Mimi Sakinah, A. M. Farhan, Mohd Said TP Chemical technology Background: The process introduced in this work is the production of sorbitol via solid state fermentation (SSF) using immobilized cells of bacteria. The raw material that was used in this study is Meranti wood sawdust (MWS) that was obtained from Kilang Kayu Aman, Gambang Kuantan, Pahang. The bacterium used was Lactobacillus plantarum (BAA-793) and was immobilized using the entrapment technique (entrapped in sodium alginate). The pretreatment processes used for the treatment of the MWS to recover the cellulose from MWS are the physical pretreatment and chemical pretreatments. The last phase of this works was fermentation using solid state (SSF) process to convert the cellulose to sorbitol. Objective: This study aim to optimize sorbitol production via solid state fermentation (SSF) process using response surface methodology (RSM) and the central composite design (CCD) was used in order to reduce the total number of experiments; besides, to determine the best combination of parameters for optimization of the process. Results: The result shows that the interactions between parameters like moisture content and substrate amount have a very significant effect and the p-value was < 0.0001. The highest yield of sorbitol production (13.607 g/L) was obtained at the condition comprised of 50 % of moisture content, 4 h of fermentation time and 1.0 gram of substrate amount. Conclusion: The production of sorbitol will increase when all the process parameters in the solid state fermentation (SSF) process have been optimized. The RSM was also suitable for fitting a quadratic surface and it also helped to optimize the effective parameters with a minimum number of experiments as well as to analyze the interaction between the parameters. AENSI Publishing 2016 Article PeerReviewed application/pdf en cc_by http://umpir.ump.edu.my/id/eprint/17288/1/ftech-2016-mimi-Optimization%20Of%20Sorbitol%20Production%20Using%20Immobilized.pdf Zuriana, Sidi Ahmad and Mimi Sakinah, A. M. and Farhan, Mohd Said (2016) Optimization Of Sorbitol Production Using Immobilized Of Lactobacillus Plantarum Strain (BAA-793) Via Solid State Fermentation (SSF) Process: Response Surface Methodology (RSM). Australian Journal of Basic and Applied Sciences, 10 (17 Sp.). pp. 90-100. ISSN 1991-8178 http://www.ajbasweb.com/old/ajbas/2016/Special%20ICCEIB/90-100.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Zuriana, Sidi Ahmad Mimi Sakinah, A. M. Farhan, Mohd Said Optimization Of Sorbitol Production Using Immobilized Of Lactobacillus Plantarum Strain (BAA-793) Via Solid State Fermentation (SSF) Process: Response Surface Methodology (RSM)
description	Background: The process introduced in this work is the production of sorbitol via solid state fermentation (SSF) using immobilized cells of bacteria. The raw material that was used in this study is Meranti wood sawdust (MWS) that was obtained from Kilang Kayu Aman, Gambang Kuantan, Pahang. The bacterium used was Lactobacillus plantarum (BAA-793) and was immobilized using the entrapment technique (entrapped in sodium alginate). The pretreatment processes used for the treatment of the MWS to recover the cellulose from MWS are the physical pretreatment and chemical pretreatments. The last phase of this works was fermentation using solid state (SSF) process to convert the cellulose to sorbitol. Objective: This study aim to optimize sorbitol production via solid state fermentation (SSF) process using response surface methodology (RSM) and the central composite design (CCD) was used in order to reduce the total number of experiments; besides, to determine the best combination of parameters for optimization of the process. Results: The result shows that the interactions between parameters like moisture content and substrate amount have a very significant effect and the p-value was < 0.0001. The highest yield of sorbitol production (13.607 g/L) was obtained at the condition comprised of 50 % of moisture content, 4 h of fermentation time and 1.0 gram of substrate amount. Conclusion: The production of sorbitol will increase when all the process parameters in the solid state fermentation (SSF) process have been optimized. The RSM was also suitable for fitting a quadratic surface and it also helped to optimize the effective parameters with a minimum number of experiments as well as to analyze the interaction between the parameters.
format	Article
author	Zuriana, Sidi Ahmad Mimi Sakinah, A. M. Farhan, Mohd Said
author_facet	Zuriana, Sidi Ahmad Mimi Sakinah, A. M. Farhan, Mohd Said
author_sort	Zuriana, Sidi Ahmad
title	Optimization Of Sorbitol Production Using Immobilized Of Lactobacillus Plantarum Strain (BAA-793) Via Solid State Fermentation (SSF) Process: Response Surface Methodology (RSM)
title_short	Optimization Of Sorbitol Production Using Immobilized Of Lactobacillus Plantarum Strain (BAA-793) Via Solid State Fermentation (SSF) Process: Response Surface Methodology (RSM)
title_full	Optimization Of Sorbitol Production Using Immobilized Of Lactobacillus Plantarum Strain (BAA-793) Via Solid State Fermentation (SSF) Process: Response Surface Methodology (RSM)
title_fullStr	Optimization Of Sorbitol Production Using Immobilized Of Lactobacillus Plantarum Strain (BAA-793) Via Solid State Fermentation (SSF) Process: Response Surface Methodology (RSM)
title_full_unstemmed	Optimization Of Sorbitol Production Using Immobilized Of Lactobacillus Plantarum Strain (BAA-793) Via Solid State Fermentation (SSF) Process: Response Surface Methodology (RSM)
title_sort	optimization of sorbitol production using immobilized of lactobacillus plantarum strain (baa-793) via solid state fermentation (ssf) process: response surface methodology (rsm)
publisher	AENSI Publishing
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/17288/ http://umpir.ump.edu.my/id/eprint/17288/ http://umpir.ump.edu.my/id/eprint/17288/1/ftech-2016-mimi-Optimization%20Of%20Sorbitol%20Production%20Using%20Immobilized.pdf
first_indexed	2023-09-18T22:23:45Z
last_indexed	2023-09-18T22:23:45Z
_version_	1777415829189558272

Optimization Of Sorbitol Production Using Immobilized Of Lactobacillus Plantarum Strain (BAA-793) Via Solid State Fermentation (SSF) Process: Response Surface Methodology (RSM)

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