GC-MS Analysis of Bioactive Constituents of Hibiscus Flower

GC-MS the best technique to identified the compounds of essential oils by comparison of mass spectra data obtained from the sample with that taken from pure commercially available standards injected under the same conditions. Objective: To characterize the chemical constituents of Hibiscus Flower us...

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Main Authors:	Rassem, Hesham H. A., Nour, A. H., R. M., Yunus
Format:	Article
Language:	English
Published:	AENSI Publishing 2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/17394/ http://umpir.ump.edu.my/id/eprint/17394/ http://umpir.ump.edu.my/id/eprint/17394/1/fkksa-2017-rosli-GC-MS%20analysis%20of%20bioactive%20constituents%20of%20Hibiscus%20flower.pdf

id	ump-17394
recordtype	eprints
spelling	ump-173942018-01-15T06:38:32Z http://umpir.ump.edu.my/id/eprint/17394/ GC-MS Analysis of Bioactive Constituents of Hibiscus Flower Rassem, Hesham H. A. Nour, A. H. R. M., Yunus TP Chemical technology GC-MS the best technique to identified the compounds of essential oils by comparison of mass spectra data obtained from the sample with that taken from pure commercially available standards injected under the same conditions. Objective: To characterize the chemical constituents of Hibiscus Flower using GC-MS. The shade dried flower powder was extracted with methanol by using Microwave-assisted Hydrodistillation (MAHD). The Agilent 5975C Series GC/MS and a DB-WAX fused silica column (30 m x 0.25 mm i.d., film thickness 0.25 μm). Oven temperature was programmed to 60 0C for 10 min, and then increased at 200C/min to 2300C and held at 2500C for 10 min. The carrier gas, helium, was adjusted to a linear velocity of 30 cm/s. The GC-MS analysis provided different peaks determining the presence of fifteen compounds. These compounds have biological activity namely Ethanimidic acid, ethyl ester (31.43%), Propanal, 2,3-dihydroxy (12.58%), 4H-Pyran-4-one, 2,3-dihydro-3,5-di hydroxy-6-methyl (10.69%), Ethylenediamine (6.71%), o-Methylisourea hydrogen sulfate (4.06%), Ethene, ethoxy- (3.63%), Methyl palmitate (2.99%), 7-Formylbicyclo[4.1.0]heptanes (2.80%), 2-Butanamine, (S)- (2.72%), 1,3,5-Triazine-2,4,6-triamine (2.48%), N-Formyl-β-alanine (2.36%), (Z)6,(Z)9-Pentadecadien-1-ol (1.70%), 1,2-Ethanediamine, Butanedial (1.65%), N-methyl-1-Propanol, 2-methyl- (1.57%) and Methanecarbothiolic acid (1.08%). From the results, it can be concluded that Hibiscus flower extract shows the presence of 15 phytocompounds. The presence of various bioactive compounds justifies the use of the whole flower for various ailments by traditional practitioners. AENSI Publishing 2017 Article PeerReviewed application/pdf en cc_by http://umpir.ump.edu.my/id/eprint/17394/1/fkksa-2017-rosli-GC-MS%20analysis%20of%20bioactive%20constituents%20of%20Hibiscus%20flower.pdf Rassem, Hesham H. A. and Nour, A. H. and R. M., Yunus (2017) GC-MS Analysis of Bioactive Constituents of Hibiscus Flower. Australian Journal of Basic and Applied Sciences, 11 (3). pp. 91-97. ISSN 1991-8178 http://ajbasweb.com/old/ajbas/2017/Special%20issue%20ICCEIB/91-97.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Rassem, Hesham H. A. Nour, A. H. R. M., Yunus GC-MS Analysis of Bioactive Constituents of Hibiscus Flower
description	GC-MS the best technique to identified the compounds of essential oils by comparison of mass spectra data obtained from the sample with that taken from pure commercially available standards injected under the same conditions. Objective: To characterize the chemical constituents of Hibiscus Flower using GC-MS. The shade dried flower powder was extracted with methanol by using Microwave-assisted Hydrodistillation (MAHD). The Agilent 5975C Series GC/MS and a DB-WAX fused silica column (30 m x 0.25 mm i.d., film thickness 0.25 μm). Oven temperature was programmed to 60 0C for 10 min, and then increased at 200C/min to 2300C and held at 2500C for 10 min. The carrier gas, helium, was adjusted to a linear velocity of 30 cm/s. The GC-MS analysis provided different peaks determining the presence of fifteen compounds. These compounds have biological activity namely Ethanimidic acid, ethyl ester (31.43%), Propanal, 2,3-dihydroxy (12.58%), 4H-Pyran-4-one, 2,3-dihydro-3,5-di hydroxy-6-methyl (10.69%), Ethylenediamine (6.71%), o-Methylisourea hydrogen sulfate (4.06%), Ethene, ethoxy- (3.63%), Methyl palmitate (2.99%), 7-Formylbicyclo[4.1.0]heptanes (2.80%), 2-Butanamine, (S)- (2.72%), 1,3,5-Triazine-2,4,6-triamine (2.48%), N-Formyl-β-alanine (2.36%), (Z)6,(Z)9-Pentadecadien-1-ol (1.70%), 1,2-Ethanediamine, Butanedial (1.65%), N-methyl-1-Propanol, 2-methyl- (1.57%) and Methanecarbothiolic acid (1.08%). From the results, it can be concluded that Hibiscus flower extract shows the presence of 15 phytocompounds. The presence of various bioactive compounds justifies the use of the whole flower for various ailments by traditional practitioners.
format	Article
author	Rassem, Hesham H. A. Nour, A. H. R. M., Yunus
author_facet	Rassem, Hesham H. A. Nour, A. H. R. M., Yunus
author_sort	Rassem, Hesham H. A.
title	GC-MS Analysis of Bioactive Constituents of Hibiscus Flower
title_short	GC-MS Analysis of Bioactive Constituents of Hibiscus Flower
title_full	GC-MS Analysis of Bioactive Constituents of Hibiscus Flower
title_fullStr	GC-MS Analysis of Bioactive Constituents of Hibiscus Flower
title_full_unstemmed	GC-MS Analysis of Bioactive Constituents of Hibiscus Flower
title_sort	gc-ms analysis of bioactive constituents of hibiscus flower
publisher	AENSI Publishing
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/17394/ http://umpir.ump.edu.my/id/eprint/17394/ http://umpir.ump.edu.my/id/eprint/17394/1/fkksa-2017-rosli-GC-MS%20analysis%20of%20bioactive%20constituents%20of%20Hibiscus%20flower.pdf
first_indexed	2023-09-18T22:23:59Z
last_indexed	2023-09-18T22:23:59Z
_version_	1777415843703947264

GC-MS Analysis of Bioactive Constituents of Hibiscus Flower

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