Production of arachidonic acid from candida krusei

The potential of arachidonic acid (AA) in pharmaceutical field had been discovered for a long time. AA is known as a fatty acid that can help information transfer of nerve systems, other than involves in growth and repair of skeletal muscle tissue. Microorganisms such as yeasts are one of the source...

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Main Author:	Nurul Izyani, Abdul Manan
Format:	Undergraduates Project Papers
Language:	English English English English
Published:	2012
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/17687/ http://umpir.ump.edu.my/id/eprint/17687/ http://umpir.ump.edu.my/id/eprint/17687/1/Production%20of%20arachidonic%20acid%20from%20candida%20krusei%20-%20Table%20of%20contents.pdf http://umpir.ump.edu.my/id/eprint/17687/7/Production%20of%20arachidonic%20acid%20from%20candida%20krusei%20-%20Abstract.pdf http://umpir.ump.edu.my/id/eprint/17687/13/Production%20of%20arachidonic%20acid%20from%20candida%20krusei%20-%20Chapter%201.pdf http://umpir.ump.edu.my/id/eprint/17687/14/Production%20of%20arachidonic%20acid%20from%20candida%20krusei%20-%20References.pdf

id	ump-17687
recordtype	eprints
spelling	ump-176872017-05-12T08:12:04Z http://umpir.ump.edu.my/id/eprint/17687/ Production of arachidonic acid from candida krusei Nurul Izyani, Abdul Manan TP Chemical technology The potential of arachidonic acid (AA) in pharmaceutical field had been discovered for a long time. AA is known as a fatty acid that can help information transfer of nerve systems, other than involves in growth and repair of skeletal muscle tissue. Microorganisms such as yeasts are one of the sources that can be commercialized as AA producer. By using microorganisms, the production process become less complicated compared to using animal source, and the ‘Halal’ standard can be fixed. The works done in this research investigate the optimum condition for AA production from yeast. In this research, the best culture condition that produced the highest yield of AA was determined by varying the pH, the carbon source and the agitation speed. The range of pH is 5 to 9. The carbon sources that were used are sucrose, starch and the mixture of sucrose and starch. Speed of incubator shaker was set at 50 rpm, 150 rpm and 250 rpm. Extraction of lipid was done by using twostep Bligh and Dyer (1959) method. Chloroform and methanol were added in specific ratio before centrifugation. The lower phase which contain lipid was collected. After evaporation, the lipid extract was dissolved in small volume of chloroform and methanol. The lipids obtained were analyzed in gas chromatography. The highest dry biomass concentration was produced at a culture at pH 7 and 250 rpm agitation rate by using sucrose while the highest yield of AA was produced at pH 7 and 150 rpm agitation rate by using sucrose as carbon source. AA production from yeast had shown advantages over other sources. Further studies on optimization of AA production should be directed at varying other significant parameters including nutrients needed and method of extraction. The findings in this study will be helpful for other research about AA in the future. 2012-01 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/17687/1/Production%20of%20arachidonic%20acid%20from%20candida%20krusei%20-%20Table%20of%20contents.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/17687/7/Production%20of%20arachidonic%20acid%20from%20candida%20krusei%20-%20Abstract.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/17687/13/Production%20of%20arachidonic%20acid%20from%20candida%20krusei%20-%20Chapter%201.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/17687/14/Production%20of%20arachidonic%20acid%20from%20candida%20krusei%20-%20References.pdf Nurul Izyani, Abdul Manan (2012) Production of arachidonic acid from candida krusei. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:66811&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nurul Izyani, Abdul Manan Production of arachidonic acid from candida krusei
description	The potential of arachidonic acid (AA) in pharmaceutical field had been discovered for a long time. AA is known as a fatty acid that can help information transfer of nerve systems, other than involves in growth and repair of skeletal muscle tissue. Microorganisms such as yeasts are one of the sources that can be commercialized as AA producer. By using microorganisms, the production process become less complicated compared to using animal source, and the ‘Halal’ standard can be fixed. The works done in this research investigate the optimum condition for AA production from yeast. In this research, the best culture condition that produced the highest yield of AA was determined by varying the pH, the carbon source and the agitation speed. The range of pH is 5 to 9. The carbon sources that were used are sucrose, starch and the mixture of sucrose and starch. Speed of incubator shaker was set at 50 rpm, 150 rpm and 250 rpm. Extraction of lipid was done by using twostep Bligh and Dyer (1959) method. Chloroform and methanol were added in specific ratio before centrifugation. The lower phase which contain lipid was collected. After evaporation, the lipid extract was dissolved in small volume of chloroform and methanol. The lipids obtained were analyzed in gas chromatography. The highest dry biomass concentration was produced at a culture at pH 7 and 250 rpm agitation rate by using sucrose while the highest yield of AA was produced at pH 7 and 150 rpm agitation rate by using sucrose as carbon source. AA production from yeast had shown advantages over other sources. Further studies on optimization of AA production should be directed at varying other significant parameters including nutrients needed and method of extraction. The findings in this study will be helpful for other research about AA in the future.
format	Undergraduates Project Papers
author	Nurul Izyani, Abdul Manan
author_facet	Nurul Izyani, Abdul Manan
author_sort	Nurul Izyani, Abdul Manan
title	Production of arachidonic acid from candida krusei
title_short	Production of arachidonic acid from candida krusei
title_full	Production of arachidonic acid from candida krusei
title_fullStr	Production of arachidonic acid from candida krusei
title_full_unstemmed	Production of arachidonic acid from candida krusei
title_sort	production of arachidonic acid from candida krusei
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/17687/ http://umpir.ump.edu.my/id/eprint/17687/ http://umpir.ump.edu.my/id/eprint/17687/1/Production%20of%20arachidonic%20acid%20from%20candida%20krusei%20-%20Table%20of%20contents.pdf http://umpir.ump.edu.my/id/eprint/17687/7/Production%20of%20arachidonic%20acid%20from%20candida%20krusei%20-%20Abstract.pdf http://umpir.ump.edu.my/id/eprint/17687/13/Production%20of%20arachidonic%20acid%20from%20candida%20krusei%20-%20Chapter%201.pdf http://umpir.ump.edu.my/id/eprint/17687/14/Production%20of%20arachidonic%20acid%20from%20candida%20krusei%20-%20References.pdf
first_indexed	2023-09-18T22:24:34Z
last_indexed	2023-09-18T22:24:34Z
_version_	1777415880520499200

Production of arachidonic acid from candida krusei

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