Recovery of β-Lagtoglobulin from whey using combination of size exclusion chromatography and ion exchange chromatography techniques

Recovery of β-Lagtoglobulin from whey using combination of size exclusion chromatography and ion exchange chromatography techniques

Variety of proteins exists in bovine whey such as a-lactalbumin (a-lac), β-lactoglobulin, (β-lg), bovine serum albumin (BSA) and immunoglobulin. The value for each individual protein can be raised if it can be isolates into a single and pure protein form. Individual whey protein hasnumerous potentia...

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Main Author: Muhammad Fuad, Abdullah
Format: Undergraduates Project Papers
Language:English
English
English
Published: 2010
Subjects:
QD Chemistry
Online Access:http://umpir.ump.edu.my/id/eprint/17792/
http://umpir.ump.edu.my/id/eprint/17792/
http://umpir.ump.edu.my/id/eprint/17792/1/Recovery%20of%20%CE%B2-Lagtoglobulin%20from%20whey%20using%20combination%20of%20size%20exclusion%20chromatography%20and%20ion%20exchange%20chromatography%20techniques%20-%20Table%20of%20contents.pdf
http://umpir.ump.edu.my/id/eprint/17792/2/Recovery%20of%20%CE%B2-Lagtoglobulin%20from%20whey%20using%20combination%20of%20size%20exclusion%20chromatography%20and%20ion%20exchange%20chromatography%20techniques%20-%20Abstract.pdf
http://umpir.ump.edu.my/id/eprint/17792/3/Recovery%20of%20%CE%B2-Lagtoglobulin%20from%20whey%20using%20combination%20of%20size%20exclusion%20chromatography%20and%20ion%20exchange%20chromatography%20techniques%20-%20Chapter%201.pdf
id ump-17792
recordtype eprints
spelling ump-177922017-05-31T04:42:19Z http://umpir.ump.edu.my/id/eprint/17792/ Recovery of β-Lagtoglobulin from whey using combination of size exclusion chromatography and ion exchange chromatography techniques Muhammad Fuad, Abdullah QD Chemistry Variety of proteins exists in bovine whey such as a-lactalbumin (a-lac), β-lactoglobulin, (β-lg), bovine serum albumin (BSA) and immunoglobulin. The value for each individual protein can be raised if it can be isolates into a single and pure protein form. Individual whey protein hasnumerous potential applications in pharmaceutical, nutraceutical and food industry. In the current study, a series of chromatographic technique was used to recover the most abundant whey protein, β-lg from the whey. In the first step, whey was separated based on it size using size exclusion chromatography (SEC). Superdex200 and Superdex75 columns were tested for SEC experiment. Superdex75 showed better peak separation compared to the Superdex200 for whey fractionation. Further polishing step was done using 1 ml HiTrap Q-Sepharose anion exchange column to fully separate a-lac and β-lg protein fraction. Different linear salt elution gradient at 5 columns volume (CV) and 10 CV were studied. I 0 CV linear salt gradient protocol gives a better separation resolution between a-lac and β-lg mixture with some fraction contain mainly pure β-lg. Recovery of single β-lg from whey can added the value of the whey-based product and it can be used for specific application. 2010-12 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/17792/1/Recovery%20of%20%CE%B2-Lagtoglobulin%20from%20whey%20using%20combination%20of%20size%20exclusion%20chromatography%20and%20ion%20exchange%20chromatography%20techniques%20-%20Table%20of%20contents.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/17792/2/Recovery%20of%20%CE%B2-Lagtoglobulin%20from%20whey%20using%20combination%20of%20size%20exclusion%20chromatography%20and%20ion%20exchange%20chromatography%20techniques%20-%20Abstract.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/17792/3/Recovery%20of%20%CE%B2-Lagtoglobulin%20from%20whey%20using%20combination%20of%20size%20exclusion%20chromatography%20and%20ion%20exchange%20chromatography%20techniques%20-%20Chapter%201.pdf Muhammad Fuad, Abdullah (2010) Recovery of β-Lagtoglobulin from whey using combination of size exclusion chromatography and ion exchange chromatography techniques. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:58089&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
English
English
topic QD Chemistry
spellingShingle QD Chemistry
Muhammad Fuad, Abdullah
Recovery of β-Lagtoglobulin from whey using combination of size exclusion chromatography and ion exchange chromatography techniques
description Variety of proteins exists in bovine whey such as a-lactalbumin (a-lac), β-lactoglobulin, (β-lg), bovine serum albumin (BSA) and immunoglobulin. The value for each individual protein can be raised if it can be isolates into a single and pure protein form. Individual whey protein hasnumerous potential applications in pharmaceutical, nutraceutical and food industry. In the current study, a series of chromatographic technique was used to recover the most abundant whey protein, β-lg from the whey. In the first step, whey was separated based on it size using size exclusion chromatography (SEC). Superdex200 and Superdex75 columns were tested for SEC experiment. Superdex75 showed better peak separation compared to the Superdex200 for whey fractionation. Further polishing step was done using 1 ml HiTrap Q-Sepharose anion exchange column to fully separate a-lac and β-lg protein fraction. Different linear salt elution gradient at 5 columns volume (CV) and 10 CV were studied. I 0 CV linear salt gradient protocol gives a better separation resolution between a-lac and β-lg mixture with some fraction contain mainly pure β-lg. Recovery of single β-lg from whey can added the value of the whey-based product and it can be used for specific application.
format Undergraduates Project Papers
author Muhammad Fuad, Abdullah
author_facet Muhammad Fuad, Abdullah
author_sort Muhammad Fuad, Abdullah
title Recovery of β-Lagtoglobulin from whey using combination of size exclusion chromatography and ion exchange chromatography techniques
title_short Recovery of β-Lagtoglobulin from whey using combination of size exclusion chromatography and ion exchange chromatography techniques
title_full Recovery of β-Lagtoglobulin from whey using combination of size exclusion chromatography and ion exchange chromatography techniques
title_fullStr Recovery of β-Lagtoglobulin from whey using combination of size exclusion chromatography and ion exchange chromatography techniques
title_full_unstemmed Recovery of β-Lagtoglobulin from whey using combination of size exclusion chromatography and ion exchange chromatography techniques
title_sort recovery of β-lagtoglobulin from whey using combination of size exclusion chromatography and ion exchange chromatography techniques
publishDate 2010
url http://umpir.ump.edu.my/id/eprint/17792/
http://umpir.ump.edu.my/id/eprint/17792/
http://umpir.ump.edu.my/id/eprint/17792/1/Recovery%20of%20%CE%B2-Lagtoglobulin%20from%20whey%20using%20combination%20of%20size%20exclusion%20chromatography%20and%20ion%20exchange%20chromatography%20techniques%20-%20Table%20of%20contents.pdf
http://umpir.ump.edu.my/id/eprint/17792/2/Recovery%20of%20%CE%B2-Lagtoglobulin%20from%20whey%20using%20combination%20of%20size%20exclusion%20chromatography%20and%20ion%20exchange%20chromatography%20techniques%20-%20Abstract.pdf
http://umpir.ump.edu.my/id/eprint/17792/3/Recovery%20of%20%CE%B2-Lagtoglobulin%20from%20whey%20using%20combination%20of%20size%20exclusion%20chromatography%20and%20ion%20exchange%20chromatography%20techniques%20-%20Chapter%201.pdf
first_indexed 2023-09-18T22:24:47Z
last_indexed 2023-09-18T22:24:47Z
_version_ 1777415893516550144

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