Effect of Flow Rate, Duty Cycle, Amplitude, and Treatment Time of Ultrasonic Regimens Towards Escherichia Coli Harbouring Lipase

A full factorial design (FFD) approach was conducted to assess the effect of four factors, namely flow rate, duty cycle, amplitude, and treatment time of ultrasonic regimens towards Escherichia coli harbouring lipase. The 22 experiments were performed as the following values with six replicates of c...

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Main Authors:	W. S. A., W. Omar, Ahmad Ziad, Sulaiman, Azilah, Ajit, Y., Chisti, A. L. T. , Chor
Format:	Conference or Workshop Item
Language:	English English
Published:	IOP Publishing 2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/18355/ http://umpir.ump.edu.my/id/eprint/18355/ http://umpir.ump.edu.my/id/eprint/18355/ http://umpir.ump.edu.my/id/eprint/18355/1/Effect%20of%20flow%20rate%2C%20duty%20cycle%2C%20amplitude%2C%20and%20treatment%20Time%20of%20ultrasonic%20regimens%20towards%20Escherichia%20coli%20harbouring%20lipase.pdf http://umpir.ump.edu.my/id/eprint/18355/2/Effect%20of%20flow%20rate%2C%20duty%20cycle%2C%20amplitude%2C%20and%20treatment%20Time%20of%20ultrasonic%20regimens%20towards%20Escherichia%20coli%20harbouring%20lipase%201.pdf

id	ump-18355
recordtype	eprints
spelling	ump-183552018-01-23T03:41:57Z http://umpir.ump.edu.my/id/eprint/18355/ Effect of Flow Rate, Duty Cycle, Amplitude, and Treatment Time of Ultrasonic Regimens Towards Escherichia Coli Harbouring Lipase W. S. A., W. Omar Ahmad Ziad, Sulaiman Azilah, Ajit Y., Chisti A. L. T. , Chor TP Chemical technology A full factorial design (FFD) approach was conducted to assess the effect of four factors, namely flow rate, duty cycle, amplitude, and treatment time of ultrasonic regimens towards Escherichia coli harbouring lipase. The 22 experiments were performed as the following values with six replicates of centre point: flow rate (0.1, 0.2, and 0.3 L/min), duty cycle (0, 20, and 40 ), amplitude (2, 6, and 10), and treatment time (10, 35, and 60 min). The FFD was employed as preliminary screening in shake flask cultivation to choose the significant factors (P< 0.05) for further optimisation process. In this study, zero duty cycle signified non-sonication of amplitude and no treatment time effect to the E. coli culture. Also, the designated flow rate and amplitude accordingly showed no effect towards the amount of dry cells weight (DCW). DCW1 was found significantly degraded after the exposure of high duty cycle and treatment time as other factors remained constant. Whereas for the lipase activity, no significant difference was observed in any main factors or interactions. Paired samples t-test confirms the result at a p-value of 0.625. This experimental study suggests the direct and continuous approach of sonication caused an adverse effect on the cells culture density. IOP Publishing 2017-06 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/18355/1/Effect%20of%20flow%20rate%2C%20duty%20cycle%2C%20amplitude%2C%20and%20treatment%20Time%20of%20ultrasonic%20regimens%20towards%20Escherichia%20coli%20harbouring%20lipase.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/18355/2/Effect%20of%20flow%20rate%2C%20duty%20cycle%2C%20amplitude%2C%20and%20treatment%20Time%20of%20ultrasonic%20regimens%20towards%20Escherichia%20coli%20harbouring%20lipase%201.pdf W. S. A., W. Omar and Ahmad Ziad, Sulaiman and Azilah, Ajit and Y., Chisti and A. L. T. , Chor (2017) Effect of Flow Rate, Duty Cycle, Amplitude, and Treatment Time of Ultrasonic Regimens Towards Escherichia Coli Harbouring Lipase. In: 29th Symposium of Malaysian Chemical Engineers (SOMChE) 2016, 1–3 December 2016 , Miri, Sarawak, Malaysia. pp. 1-11., 206 (1). ISSN 1757-8981 (Print); 1757-899X (Online) http://iopscience.iop.org/article/10.1088/1757-899X/206/1/012021/pdf DOI: 10.1088/1757-899X/206/1/012021
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology W. S. A., W. Omar Ahmad Ziad, Sulaiman Azilah, Ajit Y., Chisti A. L. T. , Chor Effect of Flow Rate, Duty Cycle, Amplitude, and Treatment Time of Ultrasonic Regimens Towards Escherichia Coli Harbouring Lipase
description	A full factorial design (FFD) approach was conducted to assess the effect of four factors, namely flow rate, duty cycle, amplitude, and treatment time of ultrasonic regimens towards Escherichia coli harbouring lipase. The 22 experiments were performed as the following values with six replicates of centre point: flow rate (0.1, 0.2, and 0.3 L/min), duty cycle (0, 20, and 40 ), amplitude (2, 6, and 10), and treatment time (10, 35, and 60 min). The FFD was employed as preliminary screening in shake flask cultivation to choose the significant factors (P< 0.05) for further optimisation process. In this study, zero duty cycle signified non-sonication of amplitude and no treatment time effect to the E. coli culture. Also, the designated flow rate and amplitude accordingly showed no effect towards the amount of dry cells weight (DCW). DCW1 was found significantly degraded after the exposure of high duty cycle and treatment time as other factors remained constant. Whereas for the lipase activity, no significant difference was observed in any main factors or interactions. Paired samples t-test confirms the result at a p-value of 0.625. This experimental study suggests the direct and continuous approach of sonication caused an adverse effect on the cells culture density.
format	Conference or Workshop Item
author	W. S. A., W. Omar Ahmad Ziad, Sulaiman Azilah, Ajit Y., Chisti A. L. T. , Chor
author_facet	W. S. A., W. Omar Ahmad Ziad, Sulaiman Azilah, Ajit Y., Chisti A. L. T. , Chor
author_sort	W. S. A., W. Omar
title	Effect of Flow Rate, Duty Cycle, Amplitude, and Treatment Time of Ultrasonic Regimens Towards Escherichia Coli Harbouring Lipase
title_short	Effect of Flow Rate, Duty Cycle, Amplitude, and Treatment Time of Ultrasonic Regimens Towards Escherichia Coli Harbouring Lipase
title_full	Effect of Flow Rate, Duty Cycle, Amplitude, and Treatment Time of Ultrasonic Regimens Towards Escherichia Coli Harbouring Lipase
title_fullStr	Effect of Flow Rate, Duty Cycle, Amplitude, and Treatment Time of Ultrasonic Regimens Towards Escherichia Coli Harbouring Lipase
title_full_unstemmed	Effect of Flow Rate, Duty Cycle, Amplitude, and Treatment Time of Ultrasonic Regimens Towards Escherichia Coli Harbouring Lipase
title_sort	effect of flow rate, duty cycle, amplitude, and treatment time of ultrasonic regimens towards escherichia coli harbouring lipase
publisher	IOP Publishing
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/18355/ http://umpir.ump.edu.my/id/eprint/18355/ http://umpir.ump.edu.my/id/eprint/18355/ http://umpir.ump.edu.my/id/eprint/18355/1/Effect%20of%20flow%20rate%2C%20duty%20cycle%2C%20amplitude%2C%20and%20treatment%20Time%20of%20ultrasonic%20regimens%20towards%20Escherichia%20coli%20harbouring%20lipase.pdf http://umpir.ump.edu.my/id/eprint/18355/2/Effect%20of%20flow%20rate%2C%20duty%20cycle%2C%20amplitude%2C%20and%20treatment%20Time%20of%20ultrasonic%20regimens%20towards%20Escherichia%20coli%20harbouring%20lipase%201.pdf
first_indexed	2023-09-18T22:25:58Z
last_indexed	2023-09-18T22:25:58Z
_version_	1777415967833325568

Effect of Flow Rate, Duty Cycle, Amplitude, and Treatment Time of Ultrasonic Regimens Towards Escherichia Coli Harbouring Lipase

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