Effects of Support Matrix for Xylanase Immobilisationon Alginate Hydrogel Beads

Enzymes serving as biocatalysts and play an important roles in many industrial field. However, the limitation of enzyme usage due to its high cost and unstable conditions of soluble enzyme to harsh conditions lead to findings an alternative to enhance the enzyme efficiency by immobilisation (insolu...

Full description

Bibliographic Details
Main Authors:	Siti Sabrina, Mohd Sukri, Mimi Sakinah, A. M.
Format:	Article
Language:	English
Published:	Penerbit Universiti Malaysia Pahang 2017
Subjects:	QD Chemistry
Online Access:	http://umpir.ump.edu.my/id/eprint/18516/ http://umpir.ump.edu.my/id/eprint/18516/ http://umpir.ump.edu.my/id/eprint/18516/ http://umpir.ump.edu.my/id/eprint/18516/1/ftech-2017-mimi-Effects%20of%20Support%20Matrix%20for%20Xylanase.pdf

id	ump-18516
recordtype	eprints
spelling	ump-185162017-10-31T04:19:38Z http://umpir.ump.edu.my/id/eprint/18516/ Effects of Support Matrix for Xylanase Immobilisationon Alginate Hydrogel Beads Siti Sabrina, Mohd Sukri Mimi Sakinah, A. M. QD Chemistry Enzymes serving as biocatalysts and play an important roles in many industrial field. However, the limitation of enzyme usage due to its high cost and unstable conditions of soluble enzyme to harsh conditions lead to findings an alternative to enhance the enzyme efficiency by immobilisation (insoluble enzyme). The present work reported a combination of immobilisation technique of xylanase by entrapment and covalent binding on alginate hydrogel beads. Xylanase enzyme was effectively immobilised within the support matrix, alginate hydrogel beads by entrapment and covalent binding on the surface of beads using glutaraldehyde as a cross-linked agent. The effects of support matrix comprised of sodium alginate concentration (% w/v) and calcium chloride, CaCl2(M) were studied in order to obtain a better immobilisation yield. The suitable concentration of sodium alginate and CaCl2 to ensure a robust and stable hydrogel beads with higher immobilisation yield were formed as a support matrix for xylanase immobilisation. The analysis of xylanase activity was determined using dinitrosalicyclic (DNS) acid reagent method. Maximal enzyme immobilisation yield (>80%) was achieved at 3.0% w/v of sodium alginate concentration and 0.3M of CaCl2. The study shows the support matrix of hydrogel beads gave a significant impact towards the immobilisation yield of xylanase. Penerbit Universiti Malaysia Pahang 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/18516/1/ftech-2017-mimi-Effects%20of%20Support%20Matrix%20for%20Xylanase.pdf Siti Sabrina, Mohd Sukri and Mimi Sakinah, A. M. (2017) Effects of Support Matrix for Xylanase Immobilisationon Alginate Hydrogel Beads. International Journal of Engineering Technology And Sciences (IJETS), 7 (1). pp. 1-8. ISSN 2289-697X http://ijets.ump.edu.my/images/archive/Vol7/4.pdf doi: 10.15282/ij ets.7.2017.1.4.1066
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Siti Sabrina, Mohd Sukri Mimi Sakinah, A. M. Effects of Support Matrix for Xylanase Immobilisationon Alginate Hydrogel Beads
description	Enzymes serving as biocatalysts and play an important roles in many industrial field. However, the limitation of enzyme usage due to its high cost and unstable conditions of soluble enzyme to harsh conditions lead to findings an alternative to enhance the enzyme efficiency by immobilisation (insoluble enzyme). The present work reported a combination of immobilisation technique of xylanase by entrapment and covalent binding on alginate hydrogel beads. Xylanase enzyme was effectively immobilised within the support matrix, alginate hydrogel beads by entrapment and covalent binding on the surface of beads using glutaraldehyde as a cross-linked agent. The effects of support matrix comprised of sodium alginate concentration (% w/v) and calcium chloride, CaCl2(M) were studied in order to obtain a better immobilisation yield. The suitable concentration of sodium alginate and CaCl2 to ensure a robust and stable hydrogel beads with higher immobilisation yield were formed as a support matrix for xylanase immobilisation. The analysis of xylanase activity was determined using dinitrosalicyclic (DNS) acid reagent method. Maximal enzyme immobilisation yield (>80%) was achieved at 3.0% w/v of sodium alginate concentration and 0.3M of CaCl2. The study shows the support matrix of hydrogel beads gave a significant impact towards the immobilisation yield of xylanase.
format	Article
author	Siti Sabrina, Mohd Sukri Mimi Sakinah, A. M.
author_facet	Siti Sabrina, Mohd Sukri Mimi Sakinah, A. M.
author_sort	Siti Sabrina, Mohd Sukri
title	Effects of Support Matrix for Xylanase Immobilisationon Alginate Hydrogel Beads
title_short	Effects of Support Matrix for Xylanase Immobilisationon Alginate Hydrogel Beads
title_full	Effects of Support Matrix for Xylanase Immobilisationon Alginate Hydrogel Beads
title_fullStr	Effects of Support Matrix for Xylanase Immobilisationon Alginate Hydrogel Beads
title_full_unstemmed	Effects of Support Matrix for Xylanase Immobilisationon Alginate Hydrogel Beads
title_sort	effects of support matrix for xylanase immobilisationon alginate hydrogel beads
publisher	Penerbit Universiti Malaysia Pahang
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/18516/ http://umpir.ump.edu.my/id/eprint/18516/ http://umpir.ump.edu.my/id/eprint/18516/ http://umpir.ump.edu.my/id/eprint/18516/1/ftech-2017-mimi-Effects%20of%20Support%20Matrix%20for%20Xylanase.pdf
first_indexed	2023-09-18T22:26:16Z
last_indexed	2023-09-18T22:26:16Z
_version_	1777415987115589632

Effects of Support Matrix for Xylanase Immobilisationon Alginate Hydrogel Beads

Similar Items